Enlisting Aero-algal Flora and Algal Allergens Using Fan Dust Sampling Method from Pune, Maharashtra

Algae in the atmosphere are one of the bio-components of the environment. Despite the fact that they may have a negative impact on human health, they are the least studied organism in the field of aerobiology. Airborne algae continue to be viable in the atmosphere in the form of spores and filaments. In order to investigate the intramural diversity of airborne algae in Pune, eight residences were chosen, from which 16 fan dust samples were collected over a ten-day period. Total (108) aero-algal forms were recorded. Out of which 107 were Cyanophyta, and only one was Chlorophyta. The dominance of Cyanophyta members is attributed to the presence of a thick mucilaginous sheath around them, which allows them to tolerate the dryness. Overall, filamentous cyanophytes (71) outnumber coccoid cyanophytes (36). However, both fan dust slide samples and culture slide samples show variance. Coccoid cyanophytes (53) outnumber filamentous cyanophytes (11) in direct slide scans, although filamentous cyanophytes (25) were somewhat more numerous in culture samples than coccoid Cyanophyta (19). This increase in filamentous forms might be because of hormogonia of filamentous algae, which remained unidentified in direct slide scan and upon inoculation underwent reproduction increasing number of filamentous algae in culture slides. Chrooccocus, Gloeocapsa, Lyngbya, Nostoc, and Stigonema were among the forms found on the first and tenth days of fan dust sampling, indicating that they were a common inhabitant of fan dust during the study and that the fan, as an aero sampler, was able to capture them, demonstrating its potency as an aero sampler. Soil can be a substantial factor in the creation of fan dust, as these genera were frequently separated from the soil. Gloeocapsa, Phormidium, Lyngbya, Schizothrix, Cylindrospermum, Nostoc, Anabaena, Scytonema, Calothrix, and Scenedesmus were among the allergic aero-algal genera discovered during the investigation.

A Pilot Study on Baseline Fungi and Moisture Indicator Fungi in Danish Homes

In many complaint cases regarding bad indoor environments, there is no evidence of visible fungal growth. To determine if the problems are fungi-related, dust sampling is the method of choice among building surveyors. However, there is a need to differentiate between species belonging to a normal, dry indoor environment and species belonging to a damp building envelope. The purposes of this pilot study were to examine which fungal species are present in problem-free Danish homes and to evaluate different detection and identification methods. Analyses showed that the fungal diversity outside was different from the diversity inside and that the composition of fungal species growing indoors was different compared to those found as spores, both indoors and outdoors. Common for most homes were Pseudopithomyceschartarum, Cladosporiumallicinum and Alternaria sect. Infectoriae together with Botrytis spp., Penicilliumdigitatum and Pen. glabrum. The results show that ITS sequencing of dust samples is adequate if supported by thorough building inspections and that food products play as large a role in the composition of the baseline spora as the outdoor air and surrounding vegetation. This pilot study provides a list of baseline fungal species found in Danish homes with a good indoor environment.

Identifying and Eliminating Laboratory Contamination by Topical Testosterone Therapeutics

Many prescription and over-the-counter drugs are available as topical formulations. Contamination of clinical laboratory workspaces by topical drugs may increase the risk of potential interference with diagnostic testing. An example of localized workspace contamination attributed to a topical hormonal drug (testosterone, T) is presented to highlight significant challenges in identifying and resolving this potential problem. Investigation included precision studies, instrument service and parts replacement, instrument replacement, airflow analysis, environmental dust sampling, and the development of customized methods for workspace monitoring and cleaning. Laboratory policies and procedures were also revised to minimize future risk.

Peningkatan Polymorphonuclear (PMN) Dalam Cairan Nasal Lavage Operator Penggilingan Padi Yang Terpajan Endotoksin Lipopolisakarida (LPS)

Background: Exposure to lipopolysaccharide endotoxin (LPS) in rice dust causes respiratory inflammation which is characterized by an increase in Neutrophils or Polymorphonuclear (PMN) in the nasal lavage fluid of rice milling operators. Objective: The purpose of this study was to analyze the effect of endotoxin LPS on the rice mill operators' PM in the rice level operators. Methods: the design of this study was a longitudinal study. Dust sampling was carried out for 8 hours while nasal lavage samples were carried out before and after work (cross shift). Endotoxin PMN and LPS were analyzed using the ELISA method and LPS Endotoxin was analyzed using LAL. Results: The mean level of LPS endotoxin in rice dust was 56.36 ± 5.83 EU / m3. An increase in post-employment PMN (Pired sample t-test: p = 0.000) in all rice milling operators. LPS endotoxin levels correlated with increased PMN in nasal lavage fluid in rice milling operators (multiple linear regression test: p = 0.000). Conclusion: LPS endotoxin is a factor that influences the increase of PMN levels in nasal lavage fluid which indicates the occurrence of inflammation in rice mill operators. Suggestion: Use a rice milling machine equipped with a dust collector. Health checks are given to the rest of their work. Workers are advised to always use masks while working.