scholarly journals Peningkatan Polymorphonuclear (PMN) Dalam Cairan Nasal Lavage Operator Penggilingan Padi Yang Terpajan Endotoksin Lipopolisakarida (LPS)

2018 ◽  
Vol 16 (1) ◽  
pp. 96-105
Author(s):  
William Wilfridus Lamawuran
Keyword(s):  
Lavage Fluid ◽  
Nasal Lavage ◽  
Dust Collector ◽  
Before And After ◽  
Background Exposure ◽  
Respiratory Inflammation ◽  
Nasal Lavage Fluid ◽  
The Mean ◽  
Rice Milling ◽  
Dust Sampling

Background: Exposure to lipopolysaccharide endotoxin (LPS) in rice dust causes respiratory inflammation which is characterized by an increase in Neutrophils or Polymorphonuclear (PMN) in the nasal lavage fluid of rice milling operators. Objective: The purpose of this study was to analyze the effect of endotoxin LPS on the rice mill operators' PM in the rice level operators. Methods: the design of this study was a longitudinal study. Dust sampling was carried out for 8 hours while nasal lavage samples were carried out before and after work (cross shift). Endotoxin PMN and LPS were analyzed using the ELISA method and LPS Endotoxin was analyzed using LAL. Results: The mean level of LPS endotoxin in rice dust was 56.36 ± 5.83 EU / m3. An increase in post-employment PMN (Pired sample t-test: p = 0.000) in all rice milling operators. LPS endotoxin levels correlated with increased PMN in nasal lavage fluid in rice milling operators (multiple linear regression test: p = 0.000). Conclusion: LPS endotoxin is a factor that influences the increase of PMN levels in nasal lavage fluid which indicates the occurrence of inflammation in rice mill operators. Suggestion: Use a rice milling machine equipped with a dust collector. Health checks are given to the rest of their work. Workers are advised to always use masks while working.

scholarly journals Galectin-10 Is Released in the Nasal Lavage Fluid of Patients with Aspirin-Sensitive Respiratory Disease

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ma. Cristina Negrete-Garcia ◽  
Carla Yoneli Jiménez-Torres ◽  
Noe Alvarado-Vásquez ◽  
A. Rosalía Montes-Vizuet ◽  
J. R. Velázquez-Rodriguez ◽  
...  
Keyword(s):  
Western Blot ◽  
Respiratory Disease ◽  
Nasal Polyps ◽  
Lavage Fluid ◽  
Nasal Lavage ◽  
Healthy Controls ◽  
Before And After ◽  
Probable Role ◽  
Nasal Lavage Fluid

The aim of this work was to determine the presence of galectin-10 in nasal lavage fluid (NLF) of patients with aspirin-sensitive respiratory disease (ASRD) before and after challenge with L-ASA (aspirin) by ELISA. Fifteen ASRD patients, ten aspirin-tolerant asthmatics (ATA), and fifteen healthy controls (HC) were studied. The baseline presence of Galectin-10 in PBMC was determined using real time PCR. Galectin-10 was evaluated in tissue of nasal polyps by western blot. Our results showed a lower expression in PBMC of ASRD patients than in ATA and healthy controls. However, a higher concentration of galectin-10 in NLF was found in ASRD patients before and after L-ASA challenge; western blot confirmed a high expression of galectin-10 in tissue from nasal polyps obtained from ASRD patients. Our results suggest a probable role of galectin-10 in the inflammatory response observed in ASRD patients; however, confirmatory studies are needed.

scholarly journals The Immuno-Modulatory Activities of Pentaherbs Formula on Ovalbumin-Induced Allergic Rhinitis Mice via the Activation of Th1 and Treg Cells and Inhibition of Th2 and Th17 Cells

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 239
Author(s):  
Peiting Li ◽  
Miranda Sin-Man Tsang ◽  
Lea Ling-Yu Kan ◽  
Tianheng Hou ◽  
Sharon Sze-Man Hon ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
T Cell ◽  
Inflammatory Mediators ◽  
Th17 Cells ◽  
Lavage Fluid ◽  
Nasal Lavage ◽  
Nasal Epithelium ◽  
Tnf Α ◽  
Nasal Lavage Fluid ◽  
Sinonasal Mucosa

Allergic rhinitis (AR) is a highly prevalent allergic disease induced by immunoglobulin (Ig) E-mediated hypersensitivity reaction at the nasal epithelium against inhaled allergens. Previous studies have demonstrated that Pentaherbs formula (PHF), a modified herbal formula comprising five herbal medicines (Flos Lonicerae, Herba Menthae, Cortex Phellodendri, Cortex Moutan and Rhizoma Atractylodis), could suppress various immune effector cells to exert anti-inflammatory and anti-allergic effects in allergic asthma and atopic dermatitis. The present study aimed to further determine the anti-inflammatory activities of PHF in an ovalbumin (OVA)-induced AR BALB/c mouse model. Nasal symptoms such as sneezing and nose rubbing were recorded and the serum total IgE and OVA-specific IgG1, as well as interleukin (IL)-4, IL-5, IL-10, IL-13, chemokines CXCL9 CXCL10, and tumor necrosis factor (TNF)-α concentrations in nasal lavage fluid (NALF) were measured during different treatments. Effects of PHF on the expression of inflammatory mediators in the sinonasal mucosa were quantified using real-time QPCR. PHF was found to suppress allergic symptoms, infiltration of inflammatory cells, and hyperplasia of goblet cells in the nasal epithelium of the OVA-induced AR mice. PHF could reduce OVA-specific IgG1 level in serum, and TNF-α and IL-10 in nasal lavage fluid (NALF), significantly up-regulate the splenic regulatory T (Treg) cell level, increase the Type 1 helper T cell (Th1)/Type 2 helper T cell (Th2) ratio, and reduce the Th17 cells (all p < 0.05). PHF could also alleviate in situ inflammation in sinonasal mucosa of OVA-induced AR mice. In conclusion, oral treatment of PHF showed immuno-modulatory activities in the OVA-induced AR mice by regulating the splenic T cell population to suppress the nasal allergy symptoms and modulating inflammatory mediators, implicating that PHF could be a therapeutic strategy for allergic rhinitis.

Cysteinyl-leukotrienes in nasal lavage fluid in children with asthma

2008 ◽  
Vol 19 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Florian Singer ◽  
Friedrich Horak ◽  
Waltraud Friesenbichler ◽  
Barbara Schneider ◽  
Zsolt Szépfalusi ◽  
...  
Keyword(s):  
Lavage Fluid ◽  
Nasal Lavage ◽  
Cysteinyl Leukotrienes ◽  
Children With Asthma ◽  
Nasal Lavage Fluid

scholarly journals Honeysuckle extract relieves ovalbumin-induced allergic rhinitis by inhibiting AR-induced inflammation and autoimmunity

2019 ◽  
Vol 39 (7) ◽  
Author(s):  
Bin Lin ◽  
Bijuan Cai ◽  
Huige Wang
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Inflammatory Reaction ◽  
Lavage Fluid ◽  
Nasal Lavage ◽  
Autoimmune Response ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Levels ◽  
Nasal Lavage Fluid ◽  
Ifn Γ

Abstract Honeysuckle has antiviral, antioxidative and anti-inflammatory properties. Allergic rhinitis (AR) is induced by immunoglobulin E (IgE)-mediated inflammatory reaction. Our study investigates whether honeysuckle extract (HE) has therapeutic effect on AR. An AR model of mice was established by ovalbumin (OVA). Hematoxylin–Eosin staining was used to assess nasal mucosa damage. Enzyme-linked immunosorbent assay (ELISA) was performed to determine serum histamine, IgE and interleukin (IL)-2, IL-4, IL-17 and interferon-γ (IFN-γ) from nasal lavage fluid. Western blot was carried out to analyze the protein level from nasal mucosa tissue. We found that HE not only decreased nasal rubbing and sneezing in AR mice, but also reduced AR-induced damage to nasal mucosa. Moreover, HE lowered the levels of serum IgE and histamine and inhibited IL-4 and IL-17 levels from AR mice but raised IL-2 and IFN-γ levels in AR-induced nasal lavage fluid. Our results also showed that HE elevated the protein levels of forkhead box P3 (Foxp3) and T-box transcription factor (T-bet) in AR-induced nasal mucosa tissue, whereas it inhibited signal transducer and activator of transcription (STAT) 3 and GATA binding protein 3 (GATA-3) protein levels. By regulating AR-induced inflammatory reaction and autoimmune response, HE also relieved OVA-induced AR. Thus, HE could be used as a potential drug to treat AR.

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