Resumen en extenso: Efectos de la inclusión de alverjón (Vicia narbonensis) en las dietas de la trucha arcoíris (Oncorhynchus mykiss): hacia una acuicultura más sostenible

Memorias del 3er Coloquio de Nutrigenómica y Biotecnología Acuícola 2020

РОЛЬ ФИЗИКО-ХИМИЧЕСКИХ СВОЙСТВ КЛЕТОЧНЫХ СТЕНОК КОРНЕЙ В ПОГЛОЩЕНИИ МЕДИ РАСТЕНИЯМИ ВИКИ НАРБОНСКОЙ

Проведен сравнительный анализ накопления меди корнями растений вики нарбонской (Vicia narbonensis L.) и изолированными из них клеточными стенками. Установлено, что основным местом накопления ионов меди у интактных растений является корневая система, а клеточные стенки корней характеризуются высоким содержанием карбоксильных групп полигалактуроновой кислоты, что обуславливает их высокую связывающую способность в отношении ионов меди. Полученные результаты показывают, что основной стратегией защиты растений вики нарбонской от повышенных концентраций меди в окружающей среде является предотвращение накопления ионов этого металла в цитоплазме клеток корня путем депонирования их в клеточную стенку.

Effects and Safe Inclusion of Narbonne Vetch (Vicia narbonensis) in Rainbow Trout (Oncorhynchus mykiss) Diets: Towards a More Sustainable Aquaculture

Aquaculture’s sustainability deeply relies on the identification and inclusion of alternative raw materials. Although meals from insects and/or byproducts from different industries are being recently tested, the meal from terrestrial vegetable species is still the main substitution candidate for fish meal. Here the effects of 0% (Control), 10% (A10) and 30% (A30) inclusion of Narbonne vetch (Vicia narbonensis; ZV-156 strain) meal in rainbow trout (Oncorhynchus mykiss) diets was assessed in a 63-day feeding trial by means of growth performance, histopathological, nutritional value of the fish fillet and blood biochemistry analyses. A dose-response trial was conducted in triplicate with 25 rainbow trout juveniles (20 g average body weight) per 500 L tank. Narbonne vetch meal decreased total intestine protease activity in vitro (from 26.81% to 48% inhibition), although high temperature partially inhibited the action of antinutritional factors (ANFs). No differences in fish growth performance and no severe histopathological alterations on the proximal intestine were observed between 10% Narbonne vetch inclusion and Control groups. In contrast, high inclusion (30%) of Narbonne vetch led to poor growth performance (30% reduction on final growth) and severe histopathological alterations (e.g., loss of brush border integrity, high number of villi fusion, reduced goblet cells density as well as reduced width of submucosa, muscular and serosa layers). Furthermore, while the A30 diet decreased docosahexaenoic fatty acid (FA) content in fish fillets, the A10 diet improved monounsaturated FA content when compared to that of the Control group. No altered levels of cholesterol, glucose or triglycerides in blood plasma and/or histopathological effects on the liver were observed among fish fed the different experimental diets. Although further research efforts (e.g., identifying potential enzymatic treatments to decrease the action of ANFs from Narbonne vetch meal) might be required, present results show that a low inclusion (10%) of Narbonne vetch in rainbow trout diets is possible. The inclusion of locally produced legumes such a Narbonne vetch might be an interesting approach to reduce carbon footprint in European aquaculture and the dependency on other alternative raw materials such as soybean (Glycine max) imported from third countries.

Determination of Quality and Chemical Composition of Silages Obtained from Narbonne Vetch (Vicia narbonensis) and Barley (Hordeum vulgare) Composition

Research was conducted to determine the quality and chemical composition of silages obtained by mixing in different ratios of narbonne vetch (Vicia narbonensis L.) and barley (Hordeum vulgare L.) grown in rabi season of 2017-2018 in Eastern Anatolia, Turkey. In the study we studied the pure sowings of barley and narbonne vetch and their mixtures (20, 30, 40, 50, 60, 70, 80% of narbonne vetch (N) + 80, 70, 60, 50, 40, 30, 20% of barley (B). The values of ADF (acid detergent fiber), NDF (neutral detergent fiber), DDM (digestible dry matter), DMI (dry matter intake), DM (dry matter), CP (crude protein) and RFV (relative feed value) of silages of analyzed were respectively 33.38, 33.73, 62.89, 3.57, 24.69, 15.50%, 174.64. We also founded that LA (lactic acid), AA (acetic acid), BA (butyric acid), PA (propionic acid) and Ca, P, Mg, K and pH of silages investigated were respectively 1.60, 0.57, 0.42, 0.06, 1.17, 0.53, 0.25, 3.51% and 3.80. Our results shows that silages with high narbonne vetch were higher quality values for CP, ADF, NDF, DM, RFV, DDM, DMI, Ca, Mg, LA which determined chemical composition and feeding quality of silages. As a result, it is suggested that mixture silages with narbonne vetch (80%) and the barley ratio (20%) have important advantages for some properties of silages.