mononucleated cell
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2020 ◽  
Vol 4 (2) ◽  
pp. 49
Author(s):  
Purwati Purwati

Acquired immune deficiency syndrome (AIDS) is caused by Human Immunodeficiency Virus (HN). At the beginning of infection, gpl20 virus interacts with CD4 receptor at the surface of the target cell. The interaction between gpI20 and CD4 leads to the occurrence of the binding of specific chemokine receptor CXCR4 and CCR5, which are also present on the membrane of the target cell.  Therefore, CCR5 arid CXCR4 also determine the fate of the target cell.  It is the performance   of CCR5 and CXCR4, guided by controlling gene that determines susceptibility or resistance to HN infection.  Coding gene CCR5 may mutate to become protective or resistant against HTV infection. In homozygote   individuals, it tends to be resistant against infection while in heterozygote individuals it tends to be susceptible to HN infection.  Objective:  To characterize   TCD4 lymphocyte in the next that is resistant against HN infection by using gene therapy deletion 32 CCR5 to use for HTV & AIDS treatment. Method: Sample collection, mononucleated cell collection, lymphocyte culture, CD4 identification, CCR5 variance analysis, co-cultivation with PBMC HN and comparison to control. Result:  This study was performed in several steps, such as mononucleated   cell isolation, followed with cell culture, lymphocyte purification, lymphocyte and CD4 expression identification.   Conclusion:  Lymphocyte T CD4 had been mature after seven passages, once passage is about 5 days so for maturity lymphocyte T CD4 need 35 days and that cell as be candidate to resistant against HN infection by using gene therapy deletion 32 CCR5 to use for HN   & AIDS treatment.





2017 ◽  
Vol 76 (1) ◽  
pp. e29-e32 ◽  
Author(s):  
Alejandro Gonzalez-Serna ◽  
Abhinav Ajaykumar ◽  
Izabelle Gadawski ◽  
Maria Angeles Muñoz-Fernández ◽  
Kanna Hayashi ◽  
...  


2010 ◽  
Vol 1 (3) ◽  
pp. 128
Author(s):  
Purwati Purwati ◽  
Nasronudin Nasronudin ◽  
Fedik Abdul Rantam

Acquired immune deficiency syndrome (AIDS) is caused by Human Immunodeficiency Virus (HIV). At the beginning of infection, gp120 virus interacts with CD4 receptor at the surface of the target cell. The interaction between gp120 and CD4 leads to the occurrence of the binding of specific chemokine receptor CXCR4 and CCR5, which are also present on the membrane of the target cell. Therefore, CCR5 and CXCR4 also determine the fate of the target cell. It is the performance of CCR5 and CXCR4, guided by controlling gene that determines susceptibility or resistance to HIV infection. Coding gene CCR5 may mutate to become protective or resistant against HIV infection. In homozygote individuals, it tends to be resistant against infection, while in heterozygote individuals it tends to be susceptible to HIV infection. Objective: To characterize TCD4 lymphocyte in the next that is resistant against HIV infection by using gene therapy deletion 32 CCR5 to use for HIV & AIDS treatment. Method: Sample collection, mononucleated cell collection, lymphocyte culture, CD4 identification, CCR5 variance analysis, co-cultivation with PBMC HIV and comparison to control. Result: This study was performed in several steps, such as mononucleated cell isolation, followed with cell culture, lymphocyte purification, lymphocyte and CD4 expression identification. Conclusion: Lymphocyte T CD4 had been mature after seven passages, once passage is about 5 days so for maturity lymphocyte T CD4 need 35 days and that cell as be candidate to resistant against HIV infection by using gene therapy deletion 32 CCR5 to use for HIV & AIDS treatment.



1986 ◽  
Vol 152 (5) ◽  
pp. 487-490 ◽  
Author(s):  
E.George Ellas ◽  
Joy M. Leuchten ◽  
Barbara S. Buda ◽  
Sally D. Brown


1983 ◽  
Vol 61 (9) ◽  
pp. 2147-2154
Author(s):  
Jean-Marie Bergeron ◽  
Louise Jodoin

In previous studies, Bergeron and Goulet and Bergeron and Jodoin examined the effects of plant secondary products on the body weight and on the weight of different organs in laboratory mice; we made a histopathological study of the liver of the same animals. Within experimental groups kept on commercial feed and on shredded plants for 6 days, mice fed on ground ivy (Glecoma hederacea), strigose fleabane (Erigeron strigosus), lesser starwort (Stellaria graminea), hop-clover (Trifolium agrarium), and common rush (Juncus effusus) suffered centro- and peri-lobular necrosis. Apart from these extreme cases, other ailments were identified: hemorrhages, pycnosis, mononucleated cell infiltrations, congestion, and vacuole formation.[Translated by the Journal]



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