Radiation-Activated Pre-Differentiated Retinal Tissue Monitored by Acoustic Wave Biosensor

A thickness-shear mode acoustic wave biosensor operated within a flow-through system was used to examine the response of mouse retinal tissue to radiation. Control experiments conducted with respect to exposure of the bare gold electrodes of the device under various conditions of light intensity and bathing solution yielded reversible changes in resonant frequency (Fs) and motional resistance (Rm). The magnitude of transient changes was proportional to light intensity, but independent of solution type. These alterations in acoustic parameters were ascribed to acoustic coupling phenomena at the electrode-to-liquid interface. Pre-differentiated retina from mouse samples deposited on the thickness shear mode (TSM) electrode exposed to a high light intensity condition also exhibited reversible changes in both Fs and Rm, compared to control experiments involving a coating used to attach the tissue to the electrode. In this case, the radiation-instigated reversible responses for both acoustic parameters exhibited a reduction in magnitude. The changes are ascribed to the alteration in viscoelasticity of the retinal matrix on the TSM electrode surface. The precise biophysical mechanism responsible for the changes in Fs and Rm remains a challenge, given the complex make up of retinal tissue.

Structural optimization and analysis of surface acoustic wave biosensor based on numerical method

The biosensor based on surface acoustic wave is of great significance for the application of biological clinical detection, but the sensitivity and specificity of surface acoustic wave biosensor still need to be improved. In this article, the structure of surface acoustic wave biosensor and interdigital transducer is designed by studying the theoretical model of surface acoustic wave biosensor. Then the amplitude–frequency response of the device is studied. Later, the simulation model of surface acoustic wave biosensor structure is established, and the performance of surface acoustic wave device is numerically calculated. Meanwhile, the relationship between the applied excitation signal and the resonant frequency is considered, and the performance of the surface wave propagation and attenuation characteristics are analyzed. In addition, the influence of the material of the piezoelectric substrate and the structure of the interdigitated electrode on the surface acoustic wave propagation are studied. The response potential curve and the total displacement of the particle vibration are analyzed, and the structure of the surface acoustic wave device is optimized.