Pumpkinseed sunfish (Lepomis gibbosus) from littoral and limnetic habitats differ in stress responsiveness independent of environmental complexity and presence of conspecifics

In the face of a changing world, there has been increasing interest in the behavioural and physiological responses of wild animals to stressors. Many factors can influence stress responsiveness, but two that have not been extensively studied during the stress-induced phase are environmental complexity and the presence of conspecifics. Using wild pumpkinseed sunfish (Lepomis gibbosus (L., 1758)) collected from limnetic and littoral sites, we tested whether glucose and cortisol were affected by environmental complexity and the density of conspecifics during the period of maximum response following a standardized air stressor. Overall, environmental complexity and conspecific density did not have a significant effect on maximum stress. However, in the environmental complexity experiment, fish collected from the littoral site had significantly higher concentrations of maximum glucose and cortisol, and tended to have higher glucose and cortisol responsiveness, than limnetic fish. This indicates that although the collection site did not affect a fish’s baseline values, intraspecific variation in site use is associated with divergent sensitivity of the hypothalamic–pituitary–interrenal axis to stressors. The importance of capture location on maximal response from stressors represents a potential sampling bias and source of variation, and may be even more pronounced in species that are habitat specialists.

Ecomorphological patterns linking morphology and diet across three populations of pumpkinseed sunfish (Lepomis gibbosus)

Relationships between morphological traits and their ecological function frequently result in patterns that are consistently observed within taxa. Across fishes, the field of ecomorphology has identified a number of morphological traits linked to foraging tactic. Here we examined the links between morphology and diet in pumpkinseed sunfish (Lepomis gibbosus (L., 1758)) from three temperate freshwater lakes. We focused on morphological variation in pharyngeal jaw and gill raker structures, both involved with the processing of prey after capture, in relation to diet. Using stomach contents and stable isotope analysis, we established mean resource use estimates for both populations and individual fish. Among populations and individuals within lakes, we observed that pharyngeal jaw size and gill raker spacing increased with the consumption of littoral prey (e.g., hard-shelled snails) relative to pelagic prey (e.g., zooplankton), but the morphological changes were greater for the pharyngeal jaws. Overall, the relationships that we observed between morphology and foraging tactic were consistent with patterns observed in pumpkinseed and across other fishes. Individual-level diet variation associated with morphology may result in phenotypic diversity within populations that has multiple ecological and evolutionary implications for these populations.