intrinsically unstructured protein
Recently Published Documents


TOTAL DOCUMENTS

34
(FIVE YEARS 1)

H-INDEX

14
(FIVE YEARS 0)

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2254
Author(s):  
Debora Bencivenga ◽  
Emanuela Stampone ◽  
Domenico Roberti ◽  
Fulvio Della Ragione ◽  
Adriana Borriello

The Cyclin-dependent kinase (CDK) regulator p27Kip1 is a gatekeeper of G1/S transition. It also regulates G2/M progression and cytokinesis completion, via CDK-dependent or -independent mechanisms. Recently, other important p27Kip1 functions have been described, including the regulation of cell motility and migration, the control of cell differentiation program and the activation of apoptosis/autophagy. Several factors modulate p27Kip1 activities, including its level, cellular localization and post-translational modifications. As a matter of fact, the protein is phosphorylated, ubiquitinated, SUMOylated, O-linked N-acetylglicosylated and acetylated on different residues. p27Kip1 belongs to the family of the intrinsically unstructured proteins and thus it is endowed with a large flexibility and numerous interactors, only partially identified. In this review, we look at p27Kip1 properties and ascribe part of its heterogeneous functions to the ability to act as an anchor or scaffold capable to participate in the construction of different platforms for modulating cell response to extracellular signals and allowing adaptation to environmental changes.



2014 ◽  
Vol 23 (8) ◽  
pp. 1013-1022 ◽  
Author(s):  
Annissa J. Huhn ◽  
Derek Parsonage ◽  
David A. Horita ◽  
Frank M. Torti ◽  
Suzy V. Torti ◽  
...  


2013 ◽  
Vol 104 (2) ◽  
pp. 5a
Author(s):  
Alexander Maltsev ◽  
Jinfa Ying ◽  
Yang Shen ◽  
Ad Bax


2012 ◽  
Vol 80 (12) ◽  
pp. 4177-4185 ◽  
Author(s):  
Christopher G. Adda ◽  
Christopher A. MacRaild ◽  
Linda Reiling ◽  
Kaye Wycherley ◽  
Michelle J. Boyle ◽  
...  

ABSTRACTMerozoite surface protein 2 (MSP2) is an abundant glycosylphosphatidylinositol (GPI)-anchored protein ofPlasmodium falciparum, which is a potential component of a malaria vaccine. As all forms of MSP2 can be categorized into two allelic families, a vaccine containing two representative forms of MSP2 may overcome the problem of diversity in this highly polymorphic protein. Monomeric recombinant MSP2 is an intrinsically unstructured protein, but its conformational properties on the merozoite surface are unknown. This question is addressed here by analyzing the 3D7 and FC27 forms of recombinant and parasite MSP2 using a panel of monoclonal antibodies raised against recombinant MSP2. The epitopes of all antibodies, mapped using both a peptide array and by nuclear magnetic resonance (NMR) spectroscopy on full-length recombinant MSP2, were shown to be linear. The antibodies revealed antigenic differences, which indicate that the conserved N- and C-terminal regions, but not the central variable region, are less accessible in the parasite antigen. This appears to be an intrinsic property of parasite MSP2 and is not dependent on interactions with other merozoite surface proteins as the loss of some conserved-region epitopes seen using the immunofluorescence assay (IFA) on parasite smears was also seen on Western blot analyses of parasite lysates. Further studies of the structural basis of these antigenic differences are required in order to optimize recombinant MSP2 constructs being evaluated as potential vaccine components.



2012 ◽  
Vol 187 (3-4) ◽  
pp. 421-430 ◽  
Author(s):  
Guiquan Guan ◽  
Emmanuelle Moreau ◽  
Junlong Liu ◽  
Miling Ma ◽  
Hélène Rogniaux ◽  
...  


2012 ◽  
Vol 33 (6) ◽  
pp. 2005-2011 ◽  
Author(s):  
Won-Kyu Lee ◽  
Sang-Yeop Lee ◽  
Jung-Hyun Na ◽  
Sung-Woo Jang ◽  
Chan-Ryang Park ◽  
...  




2011 ◽  
Vol 30 (7) ◽  
pp. 1219-1230 ◽  
Author(s):  
Jin-Ran Dai ◽  
Bing Liu ◽  
Dong-Ru Feng ◽  
Hai-yan Liu ◽  
Yan-ming He ◽  
...  


2011 ◽  
Vol 02 (04) ◽  
pp. 434-442 ◽  
Author(s):  
Swasti Raychaudhuri ◽  
Kamalika Roy Choudhury ◽  
Shreoshi Palchoudhuri ◽  
Shradha Chopra ◽  
Nitai P. Bhattacharyya ◽  
...  


Sign in / Sign up

Export Citation Format

Share Document