Genomic Insights Into the Population History and Biological Adaptation of Southwestern Chinese Hmong–Mien People

Hmong–Mien (HM) -speaking populations, widely distributed in South China, the north of Thailand, Laos, and Vietnam, have experienced different settlement environments, dietary habits, and pathogenic exposure. However, their specific biological adaptation remained largely uncharacterized, which is important in the population evolutionary genetics and Trans-Omics for regional Precision Medicine. Besides, the origin and genetic diversity of HM people and their phylogenetic relationship with surrounding modern and ancient populations are also unknown. Here, we reported genome-wide SNPs in 52 representative Miao people and combined them with 144 HM people from 13 geographically representative populations to characterize the full genetic admixture and adaptive landscape of HM speakers. We found that obvious genetic substructures existed in geographically different HM populations; one localized in the HM clines, and others possessed affinity with Han Chinese. We also identified one new ancestral lineage specifically existed in HM people, which spatially distributed from Sichuan and Guizhou in the north to Thailand in the south. The sharing patterns of the newly identified homogenous ancestry component combined the estimated admixture times via the decay of linkage disequilibrium and haplotype sharing in GLOBETROTTER suggested that the modern HM-speaking populations originated from Southwest China and migrated southward in the historic period, which is consistent with the reconstructed phenomena of linguistic and archeological documents. Additionally, we identified specific adaptive signatures associated with several important human nervous system biological functions. Our pilot work emphasized the importance of anthropologically informed sampling and deeply genetic structure reconstruction via whole-genome sequencing in the next step in the deep Chinese Population Genomic Diversity Project (CPGDP), especially in the regions with rich ethnolinguistic diversity.

Demographic model and biological adaptation inferred from the genome-wide SNP data reveal tripartite origins of southernmost Chinese Huis

The culturally unique Sanya Hui (SYH) people are regarded as the descendants of ancient Cham people in Central Vietnam (CV) and exhibit a scenario of complex migration and admixture history, who were likely to first migrate from Central and South Asia (CSA) to CV and then to South Hainan and finally assimilated with indigenous populations and resided in the tropical island environments since then. A long-standing hypothesis posits that SYH derives from different genetic and cultural origins, which hypothesizes that SYH people are different from the genetically attested admixture history of northern Hui people possessing major Han-related ancestry and minor western Eurasian ancestry. However, the effect of the cultural admixture from CSA and East Asia (EA) on the genetic admixture of SYH people remains unclear. Here, we reported the first batch of genome-wide SNP data from 94 SYH people from Hainan and comprehensively characterized their genetic structure, origin, and admixture history. Our results found that SYH people were genetically different from the northern Chinese Hui people and harbored a close genomic affinity with indigenous Vietnamese but a distinct relationship with Cham, which confirmed the hypothesis of documented recent historical migration from CV and assimilation with Hainan indigenous people. The fitted admixture models and reconstructed demographic frameworks revealed an additional influx of CSA and EA ancestries during the historical period, consisting of the frequent cultural communication along the Southern Maritime Silk Road and extensive interaction with EA. Analyses focused on natural-selected signatures of SYH people revealed a similar pattern with mainland East Asians, which further confirmed the possibility of admixture-induced biological adaptation of island environments. Generally, three genetically attested ancestries from CV, EA, and CSA in modern SYH people supported their tripartite model of genomic origins.

Genomic insights into the population history and biological adaptation of Southwestern Chinese Hmong-Mien people

Hmong-Mien-speaking (HM) populations, widely distributed in South China, North of Thailand, Laos and Vietnam, have experienced different settlement environments, dietary habits and pathogen exposure. However, their specific biological adaptation also remained largely uncharacterized, which is important in the population evolutionary genetics and Trans-Omics for regional Precision Medicine. Besides, the origin and genetic diversity of HM people and their phylogenetic relationship with surrounding modern and ancient populations are unknown. Here, we reported genome-wide SNPs in 52 representative Miao people and combined them with 144 HM people from 13 geographically representative populations to characterize the full genetic admixture and adaptive landscape of HM speakers. We found that obvious genetic substructures existed in geographically different HM populations and also identified one new ancestral lineage specifically exited in HM people, which spatially distributed from Sichuan and Guizhou in the North to Thailand in the South and temporally dated to at least 500 years. The sharing patterns of the newly-identified homogeneous ancestry component combined the estimated admixture times via the decay of Linkage Disequilibrium and haplotype sharing in GLOBETROTTER suggested that the modern HM-speaking populations originated from Southwest China and migrated southward recently, which is consistent with the reconstructed phenomena of linguistic and archeological documents. Additionally, we identified specific adaptive signatures associated with several important human nervous system biological functions. Our pilot work emphasized the importance of anthropologically-informed sampling and deeply genetic structure reconstruction via whole-genome sequencing in the next step in the deep Chinese population genomic diversity project (CPGDP), especially in the ethnolinguistic regions.

The Volumetric Properties of the Transition State Ensemble for Protein Folding

Hydrostatic pressure together with the temperature is an important environmental variable that plays an essential role in biological adaptation of extremophilic organisms. In particular, the effects of hy-drostatic pressure on the rates of the protein folding/unfolding reaction are determined by the magni-tude and sign of the activation volume changes. Here we provide computational description of the ac-tivation volume changes for folding/unfolding reaction, and compare them with the experimental data for six different globular proteins. We find that the volume of the transition state ensemble is always in-between the folded and unfolded states. Based on this, we conclude that hydrostatic pressure will invariably slow down protein folding and accelerate protein unfolding.

Non-destructive 3D microscopy in the study of patterns of biological adaptation among fossil and recent Homo

We apply non-destructive volumetric technique for evaluation of vascular net density in compact bone. Segmentation of Haversian canals and accurate estimation of their relative volume fraction gives the opportunity to estimate the density and capacity of the vascular system of the bone tissue in a proxy way. Radiological images of dorsal compact of medial and distal phalanges were studied (Neanderthals from Altai and Europe; CroMagnons; recent humans of Arctic and African origin). Results. High vascularization of compact clearly demonstrates that European Neanderthals and the majority of AMH were adapted to the Glacial Age. But Kostenki 14 (Eastern Europe) and Strashnaya 4 (Altai) have features in common with humans of recent tropic origin. Specimens of Neanderthals which lived in Altai region where glaciers never occurred are more diverse. Hypothetically the milder climate favored the existence of a wider adaptive norm reflecting patterns of former environments. Somewhat ambiguous morphological patterns of Denisova 9 and Okladnikov 2, 5 may reflect various episodes of Neanderthal migration to Siberia and their hybridization with people of tropical ancestry, most probably the early AMH. Chagyrskaya Neanderthal demonstrates a hyper-cold adaptation. Conclusion. Diversity in microstructural patterns of the Pleistocene people’s skeletal system gives evidence that both Neanderthals and CroMagnons were polymorphic in their adaptive reactions and presumably included both warm and cold adapted forms.

Allotropic Fields - On Architectural Kinetics & Biodynamic Assemblies

Through the recent prevalence of interdisciplinary design, kinetic architecture has aligned its modalities with those of biological paradigms. Homologous to transformative architecture, biological organisms exemplify a propensity for adaptation by virtue of kinetic means. Along these lines, the institution of kinetic architecture, hitherto delineated by hard mechanical means, is transitioning to soft, polymeric material systems analogous to homeostatic mechanisms. While this signals the beginning of a paradigmatic shift, architectural kinetics – nascent by its own right - finds itself only in the incipient stages of establishing a framework rooted in the operative analogies of dynamic biological behavior. With the intent of furthering this burgeoning discourse, this thesis explores the interface between mechanisms of biological adaptation and architectural kinetics in order to cultivate new transposition strategies and, in turn, develop an architectural prototype embodying novel manifestations of kinetic complexity and dynamism.

Allotropic Fields - On Architectural Kinetics & Biodynamic Assemblies

Through the recent prevalence of interdisciplinary design, kinetic architecture has aligned its modalities with those of biological paradigms. Homologous to transformative architecture, biological organisms exemplify a propensity for adaptation by virtue of kinetic means. Along these lines, the institution of kinetic architecture, hitherto delineated by hard mechanical means, is transitioning to soft, polymeric material systems analogous to homeostatic mechanisms. While this signals the beginning of a paradigmatic shift, architectural kinetics – nascent by its own right - finds itself only in the incipient stages of establishing a framework rooted in the operative analogies of dynamic biological behavior. With the intent of furthering this burgeoning discourse, this thesis explores the interface between mechanisms of biological adaptation and architectural kinetics in order to cultivate new transposition strategies and, in turn, develop an architectural prototype embodying novel manifestations of kinetic complexity and dynamism.