A Novel Family of Quadrupole-Orbitrap Mass Spectrometers for a Broad Range of Analytical Applications

The rapidly increasing adoption of high-resolution accurate-mass methods in analytical laboratories has fueled demand for instruments that combine high performance and reliability with small size and greater ease-of-use. This paper presents the major design principles that are driving the evolution of the hybrid quadrupole-Orbitrap instrument architecture to enable a greater range of applications and users. These principles may be summarized as follows: better usage of physical space and better access for service by means of size reduction of pumping and ion optics; expanded use of technologies from electronics in ion-optical design; flexibility in performance via modularity of design of the hardware and software components; and, harmonization of interfaces with other instruments to facilitate sharing and transferability of analytical workflows. The design of a novel family of hybrid mass spectrometers is described in detail, and performance evaluation is carried out on a wide variety of samples for its three representatives: the Orbitrap Exploris 120, Orbitrap Exploris 240 and Orbitrap Exploris 480 mass spectrometers.The new instrument family is shown to offer compelling potential not only for high-end proteomics and biopharmaceutical applications, but also for screening, trace, targeted and clinical analysis by liquid chromatography/mass spectrometry methods.

Rapid fabrication of ion optics by selective laser melting

Purpose Traditional ion optics manufacturing processes are complex and costly. The purpose of this paper is to study the feasibility of using selective laser melting (SLM) to produce additively manufactured ion optics. Design/methodology/approach An SLM machine was used to generate Ti6Al4V screen grids. The output was separated through wire cutting from the build platform and studied through a scanning electron microscope. To increase the geometrical accuracy of the original grid, samples consisting of nine-aperture arrays were fabricated with different parameter combinations, increasing the energy density. An empirical method to correlate the energy density applied in the fabrication process with the dimensional accuracy of the hole array positioning was developed through the analysis of multiple samples. Findings The SLM machine generated grids with optimal microstructure, the apertures fell within the specified tolerances and tolerances of slightly less than 10 µm can be guaranteed for the hole array positioning. The grids’ upper surfaces presented good-quality surface finish, and the lower surface quality was acceptable when the wire cutting process that separated the grid from the build platform performed slowly. Regardless of the build strategy, the stresses generated in the separation process caused the warping of the ion optic, so a flattening operation was necessary in all cases. Originality/value This research proved that SLM is a viable solution for ion optics fabrication, faster (less than 24 h) and less expensive (order of US$300) than traditional fabrication methods (with fabrication times from 24 to more than 400 h and costs from US$500 to US$5,000, depending on the material, size and shape).