MANAGEMENT OF ENERGY EFFICIENT PRODUCTION OF INULINASE ASPERGILLUS AWAMORI 2250

Цель работы – повышение точности и надежности управления процессом производства биомассы аэробных микроорганизмов, снижение удельных энергозатрат и повышение выхода готовой культуры. Разработан алгоритм управления процессом производства биомассы аэробных микроорганизмов. В качестве объекта производства использован ферментный препарат инулиназа, полученный глубинным способом с использованием продуцента грибов Aspergillus awamori 2250. Процесс культивирования осуществлялся в вертикальном ферментере серии Biostat (Sartorius Stedim Biotech GmbH, Германия) с рабочим объемом 100 л, предназначенным для выращивания микроорганизмов глубинным способом. Для стабилизации температурных режимов культивирования в инокуляторе, непосредственном выращивании микроорганизмов в ферментере и охлаждении готовой культуры в приемных сборниках осуществляли подготовку теплой и холодной воды с использованием пароэжекторной холодильной машины, работающей в режиме теплового насоса. Алгоритм управления производства биомассы микроорганизмов Aspergillus awamori 2250 позволил обеспечить стабилизацию параметров в области оптимальных значений и увеличить выход готовой культуры; предотвратить выброс отработанных теплоносителей в окружающую среду; обеспечить существенную экономию теплоэнергетических затрат за счет рекуперации и утилизации отработанных энергоносителей в замкнутых термодинамических циклах по материальным и энергетическим потокам. The work purpose – increase in accuracy and reliability of management of process of production of biomass of aerobic microorganisms, decrease in specific energy consumption and increase in an exit of ready culture. Algorithm of process of production of biomass of aerobic microorganisms is developed. Process of cultivation was carried out in a vertical fermenter of Sartorius Stedim Biotech of the Biostat series with the working volume of 100 liters intended for cultivation of microorganisms. For stabilization of temperature conditions of cultivation in an inokulyator, direct cultivation of culture of microorganisms in a fermenter and cooling of ready culture in reception collections carried out preparation of warm and cold water with use of the paroezhektorny refrigerator working in the thermal pump mode. The control algorithm of production of biomass of microorganisms of Aspergillus awamori 2250 has allowed to provide stabilization of parameters in the field of optimum values and to increase an exit of ready culture; to prevent emission of the fulfilled heat carriers in the environment; to provide essential economy of heat power expenses due to recovery and utilization of the fulfilled energy carriers in the closed thermodynamic cycles on material and power streams.

DYNAMICS OF CARDIOVASCULAR RISK FACTORS IN RAILWAY CREWS

Aim. To assess the prevalence of cardiovascular risk factors (RF) and dynamics over 4 years in locomotory crews.Material and methods. One hundred train drivers and assistants aged 25-59 y.o. (mean age — 43,8±10,3 y.) were investigated in-patient with 24 hour blood pressure (BP) monitoring, ultrasound Doppler of brachiocephalic arteries, standard biochemistry. Fifty three persons were assessed prospectively from 2013 to 2017 y.Results. BP increase (essential hypertension of I-II grades with mild or moderate hypertension) was found in 78 persons, with the mean duration — 10,4±4,3 years, and age of onset — 37,0±8,5 y.o. Most commonly, the dyslipidemiaswerefound:hypertriglyceridemiain59%,hypercholesterolemia in 44%. Smokers — 39%, overweight — 37%, obese — 41%. Correlational analysis revealed significant direct correlation of triglycerides with body mass index (r=0,35), with glucose tolerance disorder (r=0,22) and hypertension (r=0,22), however there was negative correlation with smoking status (r=-0,25). In patients with hypertension, aged 25-39 (n=18), comparing to the group with the none (n=22), there were significantly higher: body mass index, cholesterol level, triglycerides level and low density lipoproteideslevelwiththeabsenceofdifferenceinhighdensitylipoproteides, smoking prevalence and family anamnesis of cardiovascular diseases. In prospective follow-up the negative dynamics of lipid profile was found in males of 25-39 y.o., and morphological presentation — lesions in brachiocephalic arteries, at the age 40-49 y. with stabilization of parameters at the age 50-59 y.o. Mean group levels of systolic and diastolic BP at daytime and at night in both timepoints were within normotension range in all groups, corresponding to “non-dipper” type.Conclusion. Most prevalent RF in railway crews were dyslipidemia and obesity. The adequacy of therapy prescribed in all age strata makes it to regard the raise of BP as modifiable RF.