Theoretical study of vesicle shapes driven by coupling curved proteins and active cytoskeletal forces

Simulations show that active protrusive forces induced by curved membrane proteins enhance their aggregation and drive a transition to flat pancake-shaped vesicles. This transition resembles the sheet-like protrusions (lamellipodia) of cells.

Tank-treading as a means of propulsion in viscous shear flows

The use of tank-treading as a means of propulsion for microswimmers in viscous shear flows is taken into account. We discuss the possibility of a vesicle to control the drift in an external shear flow, by locally varying the bending rigidity of its membrane. By analytical calculation in the quasi-spherical limit, the stationary shape and the orientation of the tank-treading vesicle in the external flow are determined, working to lowest order in the membrane inhomogeneity. The membrane inhomogeneity acts in the shape evolution equation as an additional force term, which can be used to balance the effect of the hydrodynamic stresses, thus allowing the vesicle to assume shapes and orientations that are impossible otherwise. The vesicle shapes and orientations required for migration transverse to the flow, together with the bending rigidity profiles leading to such shapes and orientations, are determined. Considering the variations in the concentration experienced during tank-treading, a simple model is presented, in which a vesicle is able to migrate up or down the gradient of a concentration field by stiffening or softening of its membrane.

Shape variation of bilayer membrane daughter vesicles induced by anisotropic membrane inclusions

A theoretical model of a two-component bilayer membrane was used in order to describe the influence of anisotropic membrane inclusions on shapes of membrane daughter micro and nano vesicles. It was shown that for weakly anisotropic inclusions the stable vesicle shapes are only slightly out-of-round. In contrast, for strongly anisotropic inclusions the stable vesicle shapes may significantly differ from spheres, i.e. they have a flattened oblate shape at small numbers of inclusions in the membrane, and an elongated cigar-like prolate shape at high numbers of inclusions in the vesicle membrane.