Promotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator

Nitrogen fertiliser is manufactured using the industrial Haber–Bosch process, although it is extremely energy-consuming. One sustainable alternative technology is the electrochemical promotion of biological nitrogen fixation (BNF). This study reports the promotion of BNF activity of anaerobic microbial consortia by humin, a solid-phase humic substance, at any pH, functioning as an extracellular electron mediator, to levels of 5.7–11.8 times under nitrogen-deficient conditions. This was evidenced by increased acetylene reduction activity and total nitrogen content of the consortia. Various humins from different origins promoted anaerobic BNF activity, although the degree of promotion differed. The promotion effected by humin differed from the effects of chemical reducing agents and the effects of supplemental micronutrients and vitamins. The promotion of anaerobic BNF activity by only reduced humin without any other electron donor suggested that humin did not serve as organic carbon source but as extracellular electron mediator, for electron donation to the nitrogen-fixing microorganisms. The next generation sequencing (NGS) of partial 16S rRNA genes showed the predominance of Clostridiales (Firmicutes) in the consortia. These findings suggest the effectiveness of humin as a solid-phase extracellular electron mediator for the promotion of anaerobic BNF activity, potentially to serve for the basis for a sustainable technology.

Kinetic and deuterium isotope analyses of ammonia electrochemical synthesis

Electrochemical promotion of ammonia formation is mainly governed by surface reaction with N2 and H2 in the cathode.

Effective electrode design and the reaction mechanism for electrochemical promotion of ammonia synthesis using Fe-based electrode catalysts

Effective electrode design is investigated and a very high ammonia formation rate via EPOC is achieved using the Fe catalyst at high temperature.

Identification of the Surface Species in Electrochemical Promotion: Ethylene Oxidation over a Pt/YSZ Catalyst

The electrochemical promotion of the C2H4 + O2 total oxidation reaction over a Pt catalyst, interfaced to yttrium stabilized zirconia (YSZ), has been studied at 0.25 mbar and T =...

A Discussion on the Unique Features of Electrochemical Promotion of Catalysis (EPOC): Are We in the Right Path Towards Commercial Implementation?

The phenomenon of “Non-Faradaic Electrochemical Modification of Catalytic Activity (NEMCA)” or “Electrochemical Promotion of Catalysis (EPOC)” has been extensively studied for the last decades. Its main strength, with respect to conventionally promoted catalytic systems, is its capability to modify in-situ the activity and/or selectivity of a catalyst by controlling the supply and removal of promoters upon electrical polarization. Previous reviews have summarized the main achievements in this field from both the scientific and technological points of view. However, to this date no commercial application of the EPOC phenomenon has been developed, although numerous advances have been made on the application of EPOC on catalyst nanostructures (closer to those employed in conventional catalytic systems), and on the development of scaled-up reactors suitable for EPOC application. The main bottleneck for EPOC commercialization is likely the choice of the right chemical process. Therefore, from our point of view, future efforts should focus on coupling the latest EPOC advances with the chemical processes where the EPOC phenomenon offers a competitive advantage, either from an environmental, a practical or an economic point of view. In this article, we discuss some of the most promising cases published to date and suggest future improvement strategies. The considered processes are: (i) ethylene epoxidation with environmentally friendly promoters, (ii) NOx storage and reduction under constant reaction atmosphere, (iii) CH4 steam reforming with in-situ catalyst regeneration, (iv) H2 production, storage and release under fixed temperature and pressure, and (v) EPOC-enhanced electrolysers.