Dynamics of the habitat use of three predatory freshwater fish in a lentic ecosystem

To understand the conditions of coexistence in multiple-species predator community, we studied longitudinal and vertical movement of pike (Esox lucius), pikeperch (Sander lucioperca) and catfish (Silurus glanis) in the Rimov Reservoir, using an autonomous telemetry system for 11 months. We found significant differences among these three species in movement and depth that varied considerably in time, with the greatest differences between warm (late spring and early autumn) and cold season (late autumn to early spring). Preference for different sections of the reservoir was stable for pike, while pikeperch and catfish frequently visited tributary during the warm season, and moved closer to the dam during the cold season. Pike longitudinal activity was highest in the cold season, pikeperch in the warm season, and catfish activity peaked in both the warm and cold seasons. Overlap in the depth used among species was higher in the warm season, when all species used the upper layer of the water column, and lower in cold season, when pikeperch and catfish used deeper areas. These results demonstrated ability of predators to actively inspect a large portion of the reservoir in both longitudinal and vertical dimensions, although differing in the timing of their habitat use and activity.

Diel changes and diversity of pufM expression in freshwater communities of anoxygenic phototrophic bacteria

The anoxygenic phototrophic bacteria (APB) are an active component of aquatic microbial communities. While DNA-based studies have delivered a detailed picture of APB diversity, they cannot provide any information on the activity of individual species. Therefore, we focused on the expression of a photosynthetic gene by APB communities in two freshwater lakes (Cep lake and the Římov Reservoir) in the Czech Republic. First, we analyzed expression levels of pufM during the diel cycle using RT-qPCR. The transcription underwent a strong diel cycle and was inhibited during the day in both lakes. Then, we compared DNA- (total) and RNA-based (active) community composition by sequencing pufM amplicon libraries. We observed large differences in expression activity among different APB phylogroups. While the total APB community in the Římov Reservoir was dominated by Betaproteobacteria, Alphaproteobacteria prevailed in the active library. A different situation was encountered in the oligotrophic lake Cep where Betaproteobacteria (order Burkholderiales) dominated both the DNA and RNA libraries. Interestingly, in Cep lake we found smaller amounts of highly active uncultured phototrophic Chloroflexi, as well as phototrophic Gemmatimonadetes. Despite the large diversity of APB communities, light repression of pufM expression seems to be a common feature of all aerobic APB present in the studied lakes.

Quantification of Chaoborus and small fish by mobile upward-looking echosounding

Chaoborus larvae inhabit frequently the water column of lakes, when they can be mistaken for small fish. Because larvae ascend up to the blind zone of downward-looking echo sounding at night, quantitative acoustic estimation of them is possible only with upward-looking approach. For this reason, the mobile hydroacoustic upward-looking system (120 and 38 kHz split-beam echosounder) in combination with a direct catch method (trawling) was tested to investigate the night community of invertebrates and juvenile fish in the surface layer of the Římov reservoir (Czech Republic). In the target strength range of invertebrates (smaller than -59 dB), the 38 kHz echosounder recorded only a small proportion of targets while the 120 kHz echosounder recorded distinct peaks corresponding to high densities of Chaoborus (target strength, TS range -70 to -60 dB, average TS -66 to -64 dB). At 120 kHz frequency, the TS distribution of smaller cohort of juvenile fish (<25 mm in length) overlapped the TS-distribution of Chaoborus. The number of these smaller juvenile fish was so small compared with the number of Chaoborus that they did not seriously bias acoustic Chaoborus estimate. The correlation between the density of Chaoborus with small contamination of juvenile fish larvae from trawling and acoustic recording made with the 120 kHz echosounder was high (R2= 0.88), but the acoustic densities from trace counting appeared to underestimate Chaoborus abundance when the density was > 1.5 ind.m-3.

The Limnohabitans Genus Harbors Generalistic and Opportunistic Subtypes: Evidence from Spatiotemporal Succession in a Canyon-Shaped Reservoir

ABSTRACT We studied the diversity of Limnohabitans using reverse line blot hybridization with Limnohabitans lineage-specific probes in the freshwater canyon-shaped Římov reservoir (Czech Republic). To examine the succession of distinct lineages, we performed (i) a study of an intensive spring sampling program at the lacustrine part of the Římov reservoir (from ice melt through a phytoplankton peak to the clear-water phase), and (ii) a seasonal study (April to November) when the occurrence of distinct Limnohabitans lineages was related to the inherent longitudinal heterogeneity of the reservoir. Significant spatiotemporal changes in the compositions of distinct Limnohabitans lineages allowed for the identification of “generalists” that were always present throughout the whole season as well as “specialists” that appeared in the reservoir only for limited periods of time or irregularly. Our results indicate that some phytoplankton groups, such as cryptophytes or cyanobacteria, and zooplankton composition were the major factors modulating the distribution and dynamics of distinct Limnohabitans lineages. The highest Limnohabitans diversity was observed during the spring algal bloom, whereas the lowest was during the summer cyanobacterial bloom. The microdiversity also markedly increased upstream in the reservoir, being highest at the inflow, and thus likely reflecting strong influences of the watershed. IMPORTANCE The genus Limnohabitans is a typical freshwater bacterioplankton and is believed to play a significant role in inland freshwater habitats. This work is unique in detecting and tracing different closely related lineages of this bacterial genus in its natural conditions using the semiquantitative reverse line blot hybridization method and in discovering the factors influencing the microdiversity, subtype alternations, and seasonality.

Effects of the construction of a reservoir on the fish assemblage in an inflow river

The construction of the Římov Reservoir on the Malše River in 1978 significantly influenced the fish assemblage within the river, above the reservoir. Fish species that had successfully colonised the reservoir after impoundment expanded to the inflowing river. The changes in the riverine fish assemblage up to the first impassable weir (4 km upstream) reflected the development of the reservoir assemblage after an impoundment boom of pike (<i>Esox lucius</i>), then the perch (<i>Perca fluviatilis</i>) dominated phase and later the final cyprinid-dominated phase. The changes in the fish fauna over the period 1978–2002 were significant (&chi;², <i>P</i> < 0.001). During the cyprinid-dominated phase, roach (<i>Rutilus rutilus</i>) became the most abundant species both in the reservoir and in the river, outnumbering the pristine river species. We conclude that the cyprinid-dominated assemblage in the reservoir can be more aggressive in interfering with the river assemblage than the percid-dominated one.