Short-term effects of aligning prisms on the objective and subjective fixation disparity in far distance

Fixation disparity (FD) refers to a suboptimal condition of binocular vision. The oculomotor aspect of FD refers to a misadjustment in the vergence angle between the two visual axes that is measured in research with eye trackers (objective fixation disparity, oFD). The sensory aspect is psychophysically tested using dichoptic nonius lines (subjective fixation disparity, sFD). Some optometrists use nonius tests to determine the prisms for constant wear aiming to align the eyes. However, they do not (yet) use eye trackers. We investigate the effect of aligning prisms on oFD and sFD for 60 sec exposure duration of prisms determined with the clinically established Cross test in far distance vision. Without prisms, both types of FD were correlated with the aligning prism, while with prisms the FD was close to zero (these analyses included all base-in and base-out cases). The effect of base-in prisms on oFD was proportional to the amount of the aligning prism for the present 60 sec exposure, similar as for the 2- 5 sec exposure in Schmid et al. (2018). Thus, within 1 minute of prism exposure, no substantial vergence adaptation seems to occur in the present test conditions. Further studies may investigate intra- individual responses to different exposure times of aligning prisms in both prism directions.

On the Squall Lines Preceding Landfalling Tropical Cyclones in China

Based on a 3-yr (2007–09) mosaic of radar reflectivity and conventional surface and synoptic radiosonde observations, the general features of squall lines preceding landfalling tropical cyclones (TCs) (pre-TC) in China are examined and compared with their midlatitude and subtropical counterparts. The results show that about 40% of landfalling TCs are associated with pre-TC squall lines with high-occurring frequency in August and from late afternoon to midnight. Most pre-TC squall lines form in a broken-line mode with a trailing-stratiform organization. On average, they occur about 600 km from the TC center in the front-right quadrant with a maximum length of 220 km, a maximum radar reflectivity of 57–62 dBZ, a life span of 4 h, and a moving speed of 12.5 m s−1. Pre-TC squall lines are generally shorter in lifetime and length than typical midlatitude squall lines. Pre-TC squall lines tend to form in the transition area between the parent TC and subtropical high in a moist environment and with a weaker cold pool than their midlatitude counterparts. The environmental 0–3-km vertical shear is around 10 m s−1 and generally normal to the orientation of the squall lines. This weak shear makes pre-TC squall lines in a suboptimal condition according to the Rottuno–Klemp–Weisman (RKW) theory. Convection is likely initiated by low-level mesoscale frontogenesis, convergence, and/or confluence instead of synoptic-scale forcing. The parent TC may contribute to (i) the development of convection by enhancing conditional instability and low-level moisture supply, and (ii) the linear organization of discrete convection through the interaction between the TC and the neighboring environmental system.

Hha Is a Negative Modulator of Transcription ofhilA, the Salmonella enterica Serovar Typhimurium Invasion Gene Transcriptional Activator

ABSTRACT An early step in the establishment of Salmonella enterica serovar Typhimurium murine infection is the penetration of the intestinal mucosa of the small intestine. The majority of the genes responsible for the Salmonellainvasive phenotype are encoded on Salmonellapathogenicity island 1, and their transcription is controlled by thehilA transcriptional activator. The expression ofhilA is regulated by environmental signals including oxygen, osmolarity, pH, and growth phase such that the presence of any one suboptimal condition results in repression of hilAexpression and the invasive phenotype. We have conducted a search for negative regulators of hilA by introduction of aSalmonella enterica serovar Typhimurium chromosomal DNA gene bank into a Salmonella enterica serovar TyphimuriumhilA::Tn5lacZY reporter strain. This screen has identified the hha gene as a regulator that exerts a negative influence on hilA expression. Plasmid-encoded hha significantly reduceshilA::Tn5lacZY chromosomal expression, as well as expression of the invasion genesinvF, prgH, and sipC. Anhha null mutation results in substantial derepression of both chromosomally encoded and plasmid-encodedhilA::Tn5lacZY expression. Introduction of plasmid-encoded hha into strain SL1344 results in attenuation of invasion using in vitro and in vivo assays. Importantly, purified Hha protein was found to bind to ahilA DNA promoter fragment, suggesting that the regulatory activity of the Hha protein occurs at thehilA promoter. These data add detail to the developing model of the regulation of Salmonella invasion genes.