Petrofacies investigations of the Cretaceous Pab Formation Rakhi Gorge Eastern Sulaiman Range Pakistan - Implication for reservoir potential

Petrologic investigations of the sixty representative thin sections from the Pab Formation within Rakhi Gorge section Eastern Sulaiman Range Pakistan have been used to characterize different diagenetic patterns, identification of primary composition and reservoir characters. Sublithic, lithic and quartzose sandstones have been the principal constituents of the formation. The processes responsible for the late-stage alteration and diagenetic processes identified during the petrographic study include compaction of lithic fragments and mud clasts, formation of quartz overgrowth structures, feldspar replacement and alteration, cementation, and replacement of grains by clay minerals other ferrous elements and diagenetic minerals. The study shows that the initial porosity has been primary intergranular, but the secondary porosity has been originated in the rocks as a result of the alteration of the primary and secondary constituents, as well as fracturing. These facies characters replicate the reservoir quality including, good, medium, low-quality, and non-reservoir. Samples displaying average total porosity greater than 10 % have been declared as good quality. While rocks samples having 7 % or greater porosity are grouped as a medium reservoir. Those samples consisting of porosity values between 1 and 4% are considered as a low quality reservoir while those samples having porosity ranges low than 1% have been called non-reservoir. Based on the investigated parameters the studied samples from the Pab Formation displayed the characters of a medium reservoir that may hold significant hydrocarbon. This different quality including good and low quality zonation can be attributed to the facies and diagenetic change in the formation.

Biomarker hydrocarbons of oils from the Labagan field of the Timan-Pechora oil and gas province

The results of geochemical studies of four oils samples from Paleozoic deposits of the Labagan field of the Timan-Pechora oil and gas province are presented. It is shown that the organic matter, which generated the oil of the Lower Devonian and Tournaisian deposits of the Labagan field, is sapropelic, its accumulation occurred in a marine sub-reducing environment. The oils of the Artinskian and Ufimian deposits are substantially biodegraded. Type of collectors is fissure-porous, secondary-porous. Carbonate reservoirs of the Devonian, Carboniferous and Permian (Artinian) age are characterized by good and medium reservoir properties. Terrigenous reservoirs of the Ufimian deposit have good reservoir properties.

Research on Coupled Flow in Deformation Medium Reservoir with Cave

For the geological features of deformation medium reservoir containing cave, both Darcy seepage process in deformation medium reservoir and Navier-Stokes free flow process in cave are considered. Researching seepage flow in reservoir and free flow in cave as a whole through coupled conditions on the interface, the mathematical model of the coupled flow in the deformation medium reservoir with cave is established. For the flow characteristic of Darcy seepage and Navier-Stokes free flow, the coupling simulation is completed through balance condition of flux and pressure on the interface. The numerical solution is gained, and the chart of pressure and saturation distribution is drawn. The dynamic variation of pressure and saturation is discussed, which could describe dynamic characteristics of the coupled flow in the deformation medium reservoir with cave.

Caracterização de parâmetros hidráulicos em depósitos fluviais paleogênicos na Bacia de Volta Redonda, RJ

The present study aims for the characterization of the hydrogeological parameters of the Paleogenic fluvial deposits of Volta Redonda Geological Basin, through hydraulic conductivity determinations and grain sized analyses. The overall goal was to produce a hydrogeological data base applicable to the characterization of hydrofacies (interconnected sedimentary bodies with distinct hydraulic properties) and the modeling groundwater flow. The used methods used consisted of in situ permeability determinations (Guelph permeameters) and laboratory tests (variable head permeameter), besides grain size analyses carried out in each sedimentary facies in the study area. These sedimentary facies were characterized by Marques (2006) and belong to the Resende and Pinheiral formations. The permeameter results were coherent to the sedimentological characteristics. The Resende Formation sedimentary deposits are constituted by medium to fine sand with presence of argillaceous matrix and present moderate to very low hydraulic conductivity, varying between 10-4 to 10-8 cm/s, which indicates a poor reservoir. The Pinheiral Formation presents sandy layers with conglomeratic lenses, limited by small thickness pelitic intervals, with a very low permeability, with a hydraulic conductivity varying between 10-5 to 10-7 cm/s. The upper layer has the maximum permeability, around 10-3 cm/s. This formation presents a medium reservoir characteristics and it must be taken into consideration that the upper layer has as role in recharge to the aquifer. From the results of hydraulic conductivity, that varies from 10-8 to 10-3 cm/s for the Resende and Pinheiral formations, it is concluded that these deposits a low to medium hydraulic conductivity, explaining the low productivity of the water-bearing multilayered aquifer of Volta Redonda Basin.

NOVEL BIOREACTORS FOR OSTEOCHONDRAL TISSUE ENGINEERING

Tissue engineering is a new approach for articular cartilage repair, but the integration of engineered cartilage into the host subchondral bone is a major problem. One approach for solving this problem is to make osteochondral tissue engineering instead of cartilage tissue engineering only. The aim of the present paper was to describe two patented newly designed bioreactors for tissue-engineered osteochondral graft. The first bioreactor is double-chamber bioreactor, which is made of glass and is completely transparent. The whole system consists of one chamber for culture of chondrocytes and the other chamber for osteoblast culture. One important role for this bioreactor is to co-culture osteoblasts and chondrocytes at the same time in a biphasic scaffold. The bioreactor is modified from spinner flasks. The stirring of the magnetic bars provides medium mixing and mechanical stimulations for the cells. The second bioreactor is modified from perfusion chamber. The driven force of the medium flow is produced by siphon phenomenon. The bioreactor is composed with two parts. The first part is a modified siphon tube which can hold the biphasic scaffold for osteochondral tissue engineering. The second part is a medium reservoir bottle, which contains large amount of medium and can connect to multiple siphon tubes at the same time. The medium reservoir bottle is placed higher than the siphon tubes. The gravity will drive medium into the siphon tubes. The siphon phenomenon will make the cell-seeded scaffold covered with the medium. When the height of medium reach the height of outflow tube of the siphon tube, the medium will drain out, and the scaffold will be exposed to the air in incubator, which provides oxygen exposure. Then the gravity will make the medium refill again, the scaffold will be immersed in medium until next cycle of medium drainage out. The curve shape of the siphon tube will prevent backward bacteria contamination. The flow of the medium from reservoir through the siphon tube will produce an effect like traditional perfusion chamber bioreactor; however no power supply is necessary in this system.

Microscale Biosensor for Measurement of Volatile Fatty Acids in Anoxic Environments

ABSTRACT A microscale biosensor for acetate, propionate, isobutyrate, and lactate is described. The sensor is based on the bacterial respiration of low-molecular-weight, negatively charged species with a concomitant reduction of NO3 − to N2O. A culture of denitrifying bacteria deficient in N2O reductase was immobilized in front of the tip of an electrochemical N2O microsensor. The bacteria were separated from the outside environment by an ion-permeable membrane and supplied with nutrients (except for electron donors) from a medium reservoir behind the N2O sensor. The signal of the sensor, which corresponded to the rate of N2O production, was proportional to the supply of the electron donor to the bacterial mass. The selectivity for volatile fatty acids compared to other organic compounds was increased by selectively enhancing the transport of negatively charged compounds into the sensor by electrophoretic migration (electrophoretic sensitivity control). The sensor was susceptible to interference from O2, N2O, NO2 −, H2S, and NO3 −. Interference from NO3 − was low and could be quantified and accounted for. The detection limit was equivalent to about 1 μM acetate, and the 90% response time was 30 to 90 s. The response of the sensor was not affected by changes in pH between 5.5 and 9 and was also unaffected by changes in salinity in the range of 2 to 32‰. The functioning of the sensor over a temperature span of 7 to 30°C was investigated. The concentration range for a linear response was increased five times by increasing the temperature from 7 to 19.5°C. The life span of the biosensor varied between 1 and 3 weeks after manufacturing.