COMPARATIVE EVALUATION OF THE RESULTS OF IMMUNOFLUORESCENCE AND HEMOFILTROCYTOLOGICAL RESEARCH IN CANCER PATIENTS

Immunofluorescence is a unique immunochemical method that combines the ability to conduct a detailed morphological analysis and use the specificity of the immunological reaction. The advantage of the immunofluorescence method is its high sensitivity and resolution. The immunofluorescence method was used to survey cell films of venous blood filtrate of cancer patients, and with usage of an apparatus for blood micro-screening, the successful extraction of circulating cancer cells was confirmed.

VOLATILE FATTY ACID AND LACTIC ACID CONTENT OF PIG BLOOD

Carotid and portal blood from each of three 30-ltg pigs was analysed for volatile fatty acids and lactic acid. Propionic, acetic, and formic acids were found in all samples. Butyric acid was not detected in carotid blood and only in the portal blood from two of the pigs. Average molar proportions of the individual acids in portal blood were: butyric, 0.5; propionic, 1.7; acetic, 16.3; formic, 24.6; and lactic, 56.9% with a total concentration of 1.06 mmole per 100 ml blood. A possible energy contribution by acetic, propionic, and butyric acids in the pig was calculated to be between 184 and 330 kcal daily or 15 and 28% of the maintenance energy requirement. Tests on blood preparation and duration of frozen storage demonstrated the advisability of using a deproteinized blood filtrate when storage periods of up to 12 days were necessary.

A New Simple Semimicro Method for Colorimetric Determination of Urea

A new simple and rapid method for quantitative semimicro determination of blood urea is presented. Diacetyl monoxime and thiosemicarbazide form in acid medium with urea a red color complex with a maximum adsorption at 530 mµ stable at room temperature and requiring 0.2 ml. of 1:10 blood filtrate.