5-Nitroisatin-4-(1-(2-pyridyl)piperazinyl)-3-thiosemicarbazone (Nitistpyrdlpz) and its Cu(II) complex
were synthesized and characterized by CHN and thermal analysis and spectroscopic measurements
viz. UV-vis, FTIR, 1H NMR, 13C NMR, ESI-HRMS, PXRD and EPR. In the complex, copper(II) ion
is coordinated by terdentate thiosemicarbazone anion and one chloride ion in a distorted square planar
geometry. The synthesized compounds against breast cancer cell lines; MCF-7 and MDA-MB-231
and epidermoid carcinoma; A431 showed that the complex contributed to reduce the percentage of
cell viability toward all the tested cell lines but remarkable contribution toward MDA-MB-231 cell
line. The IC50 of the complex and free ligand was found in the range of IC50 0.85-1.24 μM and IC50
3.28-3.53 μM, respectively. Among those cell lines, the complex may be the better anticancer agent
toward MDA-MB-231 because of its action at micromolar concentration (IC50 0.85 μM).