glycerinated fibers
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1999 ◽  
Vol 39 (supplement) ◽  
pp. S191
Author(s):  
T. Sekine ◽  
T. Yamada


1996 ◽  
Vol 271 (3) ◽  
pp. C878-C886 ◽  
Author(s):  
R. W. Wiseman ◽  
T. W. Beck ◽  
P. B. Chase

The cellular mechanism of muscle fatigue is still in debate. Opposite conclusions regarding the role of intracellular pH (pHi) in fatigue have been drawn from skinned fiber vs. isolated perfused muscle studies. Because these experiments are typically performed at different temperatures, we tested the hypothesis that temperature alters the effects of pH on force. Tetanic force of isolated mouse extensor digitorum longus was measured at temperatures between 13 and 25 degrees C in either normocapnia (5% CO2) or hypercapnia (25% CO2). Hypercapnia decreased pHi (monitored by 31P nuclear magnetic resonance spectroscopy) by the same amount at both 15 and 25 degrees C. However, inhibition of force by hypercapnia was greater at the lower temperature. A similar pattern of temperature-dependent inhibition of force by pH was observed in glycerinated fibers from rabbit psoas at maximum Ca2+ activation. We conclude that temperature differences are responsible for disparate conclusions on the role of pHi in muscle fatigue. Based on our results, we suggest that changes in pHi may have little or no role in the loss in force production associated with muscular fatigue at physiological temperatures.







1980 ◽  
Vol 88 (3) ◽  
pp. 909-911 ◽  
Author(s):  
Hiroshi SUZUKI ◽  
Kunihiko KONNO ◽  
Ken-ichi ARM ◽  
Shizuo WATANABE


1976 ◽  
Vol 71 (1) ◽  
pp. 261-272 ◽  
Author(s):  
M Elfvin ◽  
R J Levine ◽  
M M Dewey

By sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunodiffusion, we identified paramyosin in two smooth invertebrate "catch" muscles (Mytilus anterior byssus retractor and Mercenaria opaque adductor) and five invertebrate striated muscles (Limulus telson levator, Homarus claw muscle, Balanus scutal depressor, Lethocerus air tube retractor, and Aequipecten striated adductor). We show that (a) the paramyosins in all of these muscles have the same chain weights and (b) they are immunologically similar. We stained all of these muscles with specific antibody to Limulus paramyosin using the indirect fluorescent antibody technique. Paramyosin was localized to the A bands of the glycerinated striated muscles, and diffus fluorescence was seen throughout the glycerinated fibers of the smooth catch muscles. The presence of paramyosin in Homarus claw muscle, Balanus scutal depressor, and Lethocerus air tube retractor is shown here for the first time. Of the muscles in this study, Limulus telson levator is the only one for which the antiparamyosin staining pattern has been previously reported.



1972 ◽  
Vol 31 (5) ◽  
pp. 740-749 ◽  
Author(s):  
PHILIP D. HENRY ◽  
Gail G. AHUMADA ◽  
WILLIAM F. FRIEDMAN ◽  
BURTON E. SOBEL


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