Continuous Particle Separation Driven by 3D Ag-PDMS Electrodes with Dielectric Electrophoretic Force Coupled with Inertia Force

Cell separation has become @important in biological and medical applications. Dielectrophoresis (DEP) is widely used due to the advantages it offers, such as the lack of a requirement for biological markers and the fact that it involves no damage to cells or particles. This study aimed to report a novel approach combining 3D sidewall electrodes and contraction/expansion (CEA) structures to separate three kinds of particles with different sizes or dielectric properties continuously. The separation was achieved through the interaction between electrophoretic forces and inertia forces. The CEA channel was capable of sorting particles with different sizes due to inertial forces, and also enhanced the nonuniformity of the electric field. The 3D electrodes generated a non-uniform electric field at the same height as the channels, which increased the action range of the DEP force. Finite element simulations using the commercial software, COMSOL Multiphysics 5.4, were performed to determine the flow field distributions, electric field distributions, and particle trajectories. The separation experiments were assessed by separating 4 µm polystyrene (PS) particles from 20 µm PS particles at different flow rates by experiencing positive and negative DEP. Subsequently, the sorting performances of the 4 µm PS particles, 20 µm PS particles, and 4 µm silica particles with different solution conductivities were observed. Both the numerical simulations and the practical particle separation displayed high separating efficiency (separation of 4 µm PS particles, 94.2%; separation of 20 µm PS particles, 92.1%; separation of 4 µm Silica particles, 95.3%). The proposed approach is expected to open a new approach to cell sorting and separating.

On particle separation from turbulent particle plumes in a cross-flow

We present new experiments of particle-driven turbulent plumes issuing from a constant source of dense particle-laden fluid, with buoyancy flux, $B$ , in a uniform horizontal current, $u$ . Experiments show that a turbulent, well-mixed plume develops, in which the downward vertical speed $w$ decreases with depth $z$ according to $w = 0.76 (B/uz)^{1/2}$ while the horizontal speed rapidly asymptotes to the current speed $u$ , provided that the Stokes settling speed of the particles $v<0.92 w$ . For $v > 0.92 w$ , the particles separate from the plume fluid, and their depth $z$ increases according to the simple sedimentation trajectory $\textrm {d}z/{\textrm {d}\kern0.7pt x} = v/u$ . As the particles sediment, they form clusters of particles, which lead to fluctuations in the particle load with position, but do not appear to change the time-average sedimentation speed. We explore the impact of these results for deep-sea mining, in which the fate of the plume water as well as the particles is key for assessing potential environmental impacts.