dsp toxins
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2021 ◽  
pp. 105950
Author(s):  
Jia-hui Huang ◽  
Yu-hu Jiao ◽  
Li Li ◽  
Da-wei Li ◽  
Hong-ye Li ◽  
...  


2021 ◽  
Vol 9 (3) ◽  
pp. 293
Author(s):  
Mauro Corriere ◽  
Lucía Soliño ◽  
Pedro Reis Costa

Natural high proliferations of toxin-producing microorganisms in marine and freshwater environments result in dreadful consequences at the socioeconomically and environmental level due to water and seafood contamination. Monitoring programs and scientific evidence point to harmful algal blooms (HABs) increasing in frequency and intensity as a result of global climate alterations. Among marine toxins, the okadaic acid (OA) and the related dinophysistoxins (DTX) are the most frequently reported in EU waters, mainly in shellfish species. These toxins are responsible for human syndrome diarrhetic shellfish poisoning (DSP). Fish, like other marine species, are also exposed to HABs and their toxins. However, reduced attention has been given to exposure, accumulation, and effects on fish of DSP toxins, such as OA. The present review intends to summarize the current knowledge of the impact of DSP toxins and to identify the main issues needing further research. From data reviewed in this work, it is clear that exposure of fish to DSP toxins causes a range of negative effects, from behavioral and morphological alterations to death. However, there is still much to be investigated about the ecological and food safety risks related to contamination of fish with DSP toxins.



2020 ◽  
Vol 524 ◽  
pp. 151317
Author(s):  
Raquel A.F. Neves ◽  
Silvia M. Nascimento ◽  
Luciano N. Santos


2019 ◽  
Vol 176 ◽  
pp. 178-185 ◽  
Author(s):  
Xiao-Meng Wei ◽  
Mi-Yu Lu ◽  
Guo-Fang Duan ◽  
Hong-Ye Li ◽  
Jie-Sheng Liu ◽  
...  


Toxins ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 417 ◽  
Author(s):  
María Verónica Prego-Faraldo ◽  
Luisa Martínez ◽  
Josefina Méndez

The harmful effects of diarrhetic shellfish poisoning (DSP) toxins on mammalian cell lines have been widely assessed. Studies in bivalves suggest that mussels display a resistance to the cytogenotoxic effects of DSP toxins. Further, it seems that the bigger the exposure, the more resistant mussels become. To elucidate the early genetic response of mussels against these toxins, the digestive gland and the gill transcriptomes of Mytilus galloprovincialis after Prorocentrum lima exposure (100,000 cells/L, 48 h) were de novo assembled based on the sequencing of 8 cDNA libraries obtained using an Illumina HiSeq 2000 platform. The assembly provided 95,702 contigs. A total of 2286 and 4523 differentially expressed transcripts were obtained in the digestive gland and the gill, respectively, indicating tissue-specific transcriptome responses. These transcripts were annotated and functionally enriched, showing 44 and 60 significant Pfam families in the digestive gland and the gill, respectively. Quantitative PCR (qPCR) was performed to validate the differential expression patterns of several genes related to lipid and carbohydrate metabolism, energy production, genome integrity and defense, suggesting their participation in the protective mechanism. This work provides knowledge of the early response against DSP toxins in the mussel M. galloprovincialis and useful information for further research on the molecular mechanisms of the bivalve resistance to these toxins.



2018 ◽  
Vol 49 (2) ◽  
pp. 743-757 ◽  
Author(s):  
Paula Abal ◽  
M. Carmen Louzao ◽  
Toshiyuki Suzuki ◽  
Ryuichi Watanabe ◽  
Natalia Vilariño ◽  
...  

Background/Aims: Okadaic acid (OA) and the structurally related compounds dinophysistoxin-1 (DTX1) and dinophysistoxin-2 (DTX2) are marine phycotoxins that cause diarrheic shellfish poisoning (DSP) in humans due to ingestion of contaminated shellfish. In order to guarantee consumer protection, the regulatory authorities have defined the maximum level of DSP toxins as 160 µg OA equivalent kg-1 shellfish meat. For risk assessment and overall toxicity determination, knowledge of the relative toxicities of each analogue is required. In absence of enough information from human intoxications, oral toxicity in mice is the most reliable data for establishing Toxicity Equivalence Factors (TEFs). Methods: Toxins were administered to mice by gavage, after that the symptomatology and mice mortality was registered over a period of 24 h. Organ damage data were collected at necropsy and transmission electron microscopy (TEM) was used for ultrastructural studies. Toxins in urine, feces and blood were analyzed by HPLC-MS/MS. The evaluation of in vitro potencies of OA, DTX1 and DTX2 was performed by the protein phosphatase 2A (PP2A) inhibition assay. Results: Mice that received DSP toxins by gavage showed diarrhea as the main symptom. Those toxins caused similar gastrointestinal alterations as well as intestine ultrastructural changes. However, DSP toxins did not modify tight junctions to trigger diarrhea. They had different toxicokinetics and toxic potency. The lethal dose 50 (LD50) was 487 µg kg-1 bw for DTX1, 760 µg kg-1 bw for OA and 2262 µg kg-1 bw for DTX2. Therefore, the oral TEF values are: OA = 1, DTX1 = 1.5 and DTX2 = 0.3. Conclusion: This is the first comparative study of DSP toxins performed with accurate well-characterized standards and based on acute toxicity data. Results confirmed that DTX1 is more toxic than OA by oral route while DTX2 is less toxic. Hence, the current TEFs based on intraperitoneal toxicity should be modified. Also, the generally accepted toxic mode of action of this group of toxins needs to be reevaluated.





Harmful Algae ◽  
2017 ◽  
Vol 63 ◽  
pp. 85-93 ◽  
Author(s):  
Tingmo Hu ◽  
Patricia LeBlanc ◽  
Ian W. Burton ◽  
John A. Walter ◽  
Pearse McCarron ◽  
...  


2017 ◽  
Vol 83 (3) ◽  
pp. 367-372 ◽  
Author(s):  
TSUYOSHI IKEHARA ◽  
TSUBASA KINOSHITA ◽  
AYAKA KUROKAWA ◽  
SHIHOKO NAKASHIMA ◽  
KIMIHIKO MAEKAWA ◽  
...  


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