aqp4 protein
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2020 ◽  
Vol 10 (4) ◽  
pp. 563-570
Author(s):  
Zhengang Sun ◽  
Xinchao Zhang ◽  
Lingyun Hu ◽  
Xiaoshuai Wang ◽  
Zhenjuan Sun ◽  
...  

This study was to determine whether Jasplakinolide (JSK), an actin microfilament polymerization, reduced the astrocytic swelling induced by oxygen-glucose deprivation and reoxygenation (OGD/Reox) and whether this effect could be mediated through the modulation of aquaporin-4 (AQP4) expression. Astrocytes were obtained from rat spinal cord. Cells were subjected to reoxygenation after 5 h OGD. JSK with different concentrations (20, 50, 100, 200 and 400 nM) was added to astrocytes for 12 h before and during OGD/Reox. Then the cell volume and AQP4 expression were analyzed. The results showed that astrocytic swelling occurred with cell volume increasing after OGD/Reox and the severity was significantly attenuated by JSK. A strong increase of AQP4 protein expression was observed after OGD/Reox, reaching a peak at 1.5 h into reoxygenation. The increase in levels of AQP4 protein was significantly reduced by treatment with JSK. In addition, confocal microscopy analysis showed that the F-actin microfilament polymerization by JSK accounted for AQP4 downregulation in the plasma membrane. This study demonstrated that JSK could attenuate astrocytes swelling caused by OGD/Reox, an ischemia and reperfusion-like injury, and part of its protective effects might be related to the reorganization the F-actin microfilament and inhibiting the abundance expression of AQP4 protein.



2011 ◽  
Vol 301 (1) ◽  
pp. C204-C212 ◽  
Author(s):  
Jennifer M. Rutkowsky ◽  
Breanna K. Wallace ◽  
Phyllis M. Wise ◽  
Martha E. O'Donnell

In the early hours of ischemic stroke, cerebral edema forms as Na, Cl, and water are secreted across the blood-brain barrier (BBB) and astrocytes swell. We have shown previously that ischemic factors, including hypoxia, aglycemia, and arginine vasopressin (AVP), stimulate BBB Na-K-Cl cotransporter (NKCC) and Na/H exchanger (NHE) activities and that inhibiting NKCC and/or NHE by intravenous bumetanide and/or HOE-642 reduces edema and infarct in a rat model of ischemic stroke. Estradiol also reduces edema and infarct in this model and abolishes ischemic factor stimulation of BBB NKCC and NHE. There is evidence that NKCC and NHE also participate in ischemia-induced swelling of astrocytes. However, little is known about estradiol effects on astrocyte cell volume. In this study, we evaluated the effects of AVP (100 nM), hypoxia (7.5% O2), aglycemia, hypoxia (2%)/aglycemia [oxygen glucose deprivation (OGD)], and estradiol (1–100 nM) on astrocyte cell volume using 3- O-methyl-d-[3H]glucose equilibration methods. We found that AVP, hypoxia, aglycemia, and OGD (30 min to 5 h) each significantly increased astrocyte cell volume, and that estradiol (30–180 min) abolished swelling induced by AVP or hypoxia, but not by aglycemia or OGD. Bumetanide and/or HOE-642 also abolished swelling induced by AVP but not aglycemia. Abundance of aquaporin-4, known to participate in ischemia-induced astrocyte swelling, was significantly reduced following 7-day but not 2- or 3-h estradiol exposures. Our findings suggest that hypoxia, aglycemia, and AVP each contribute to ischemia-induced astrocyte swelling, and that the edema-attenuating effects of estradiol include reduction of hypoxia- and AVP-induced astrocyte swelling and also reduction of aquaporin-4 abundance.



2008 ◽  
Vol 1195 ◽  
pp. 1-11 ◽  
Author(s):  
MoonHee Lee ◽  
Sung-Jin Lee ◽  
Hyo-Jung Choi ◽  
Yong-Wook Jung ◽  
Jørgen Frøkiær ◽  
...  


2005 ◽  
Vol 17 (5) ◽  
pp. 535 ◽  
Author(s):  
Nitin P. Ron ◽  
John A. Kazianis ◽  
James F. Padbury ◽  
Courtney M. Brown ◽  
Bethany G. McGonnigal ◽  
...  

The aim of the present study was to determine the ontogeny and effects of corticosteroid pretreatment on aquaporin 4 (AQP4) channel mRNA and protein expression in the cerebral cortex of sheep during development. A portion of the cerebral cortex was snap-frozen from fetuses of dexamethasone- and placebo-treated ewes at 60%, 80% and 90% of gestation, dexamethasone- and placebo-treated newborn lambs and adult sheep. Cerebral cortical samples were obtained 18 h after the last of four 6 mg dexamethasone or placebo injections were given over 48 h to the ewes and adult sheep. Lambs were treated with 0.01 mg kg−1 dexamethasone or placebo in the same schedule as the ewes and adult sheep. Amplification of an ovine AQP4 cDNA fragment was accomplished by reverse transcription–polymerase chain reaction using primers based on a homologous bovine sequence. The resulting cDNA was used to determine AQP4 channel mRNA expression by Northern hybridisation using phosphorimaging. The relative abundance of AQP4 mRNA was normalised to the ovine ribosomal gene L32. A portion of the frontal cortex was also analysed for AQP4 protein expression by Western immunoblot. Densitometry was performed and the results expressed as a ratio to an adult brain pool. Aquaporin 4 channel mRNA and protein were detectable as early as at 60% gestation. There were no changes in AQP4 mRNA expression among the fetal, newborn and adult groups or after dexamethasone pretreatment in any age group. The expression of the AQP4 protein was higher (P < 0.05) in fetuses at 80% and 90% of gestation (2.9- and 3.3-fold, respectively), in lambs (3.2-fold) and in adult sheep (3.8-fold) compared with fetuses at 60% of gestation, as well as in adult sheep (1.3-fold) compared with fetuses at 80% of gestation. Dexamethasone pretreatment resulted in decreases (P < 0.05) in AQP4 protein expression in the lambs and adult sheep, but not in the fetal groups. We conclude that: (1) AQP4 mRNA and protein were expressed early in fetal and throughout ovine development; (2) protein, but not mRNA, expression increased between 60% and 80% of gestation and did not differ from adult levels by 90% of gestation; and (3) dexamethasone pretreatment resulted in decreases in AQP4 protein expression in lambs and adult sheep, but not in fetuses. The maturational increases in AQP4 protein expression and dexamethasone-related decreases in expression were post-transcriptional, because changes in AQP4 mRNA expression were not observed.



2001 ◽  
Vol 280 (5) ◽  
pp. F794-F802 ◽  
Author(s):  
Yan Huang ◽  
Randall Tracy ◽  
Glenn E. Walsberg ◽  
Anthony Makkinje ◽  
Pingke Fang ◽  
...  

Recently, we found that aquaporin-4 (AQP4) is expressed in the S3 segment of renal proximal tubules of mice but not in rat proximal tubules. Because mice have relatively larger papillae than rats, it was proposed that the renal distribution of AQP4 in various species could be related to their maximum urinary concentrating ability. Therefore, kidneys and other tissues of Merriam's desert kangaroo rat, Dipodomys merriami merriami, which produce extremely concentrated urine (up to 5,000 mosmol/kgH2O), were examined for AQP4 expression and localization. Contrary to our expectation, AQP4 immunostaining was undetectable in any region of the kidney, and the absence of AQP4 protein was confirmed by Western blotting. By freeze fracture electron microscopy, orthogonal arrays of intramembraneous particles (OAPs) were not detectable in plasma membranes of principal cells and proximal tubules. However, AQP4 protein was readily detectable in gastric parietal and brain astroglial cells. Northern blotting failed to detect AQP4 mRNA in kangaroo rat kidneys, whereas both in situ hybridization and RT-PCR experiments did reveal AQP4 mRNA in collecting ducts and proximal tubules of the S3 segment. These results suggest that renal expression of AQP4 in the kangaroo rat kidney is regulated at the transcriptional or translational level, and the absence of AQP4 may be critical for the extreme urinary concentration that occurs in this species.



2000 ◽  
Vol 279 (2) ◽  
pp. G448-G453 ◽  
Author(s):  
Kasper S. Wang ◽  
Alex R. Komar ◽  
Tonghui Ma ◽  
Ferda Filiz ◽  
Jeff McLeroy ◽  
...  

The aquaporin-4 (AQP4) water channel has been proposed to play a role in gastric acid secretion. Immunocytochemistry using anti-AQP4 antibodies showed strong AQP4 protein expression at the basolateral membrane of gastric parietal cells in wild-type (+/+) mice. AQP4 involvement in gastric acid secretion was studied using transgenic null (−/−) mice deficient in AQP4 protein. −/− Mice had grossly normal growth and appearance and showed no differences in gastric morphology by light microscopy. Gastric acid secretion was measured in anesthetized mice in which the stomach was luminally perfused (0.3 ml/min) with 0.9% NaCl containing [14C]polyethylene glycol ([14C]PEG) as a volume marker. Collected effluent was assayed for titratable acid content and [14C]PEG radioactivity. After 45-min baseline perfusion, acid secretion was stimulated by pentagastrin (200 μg · kg−1· h−1 iv) for 1 h or histamine (0.23 mg/kg iv) + intraluminal carbachol (20 mg/l). Baseline gastric acid secretion (means ± SE, n = 25) was 0.06 ± 0.03 and 0.03 ± 0.02 μeq/15 min in +/+ and −/− mice, respectively. Pentagastrin-stimulated acid secretion was 0.59 ± 0.14 and 0.70 ± 0.15 μeq/15 min in +/+ and −/− mice, respectively. Histamine plus carbachol-stimulated acid secretion was 7.0 ± 1.9 and 8.0 ± 1.8 μeq/15 min in +/+ and −/− mice, respectively. In addition, AQP4 deletion did not affect gastric fluid secretion, gastric pH, or fasting serum gastrin concentrations. These results provide direct evidence against a role of AQP4 in gastric acid secretion.



2000 ◽  
Vol 278 (6) ◽  
pp. C1108-C1115 ◽  
Author(s):  
Baoxue Yang ◽  
Jean-Marc Verbavatz ◽  
Yuanlin Song ◽  
L. Vetrivel ◽  
Geoffrey Manley ◽  
...  

It has been proposed that aquaporin-4 (AQP4), a water channel expressed at the plasmalemma of skeletal muscle cells, is important in normal muscle physiology and in the pathophysiology of Duchenne's muscular dystrophy. To test this hypothesis, muscle water permeability and function were compared in wild-type and AQP4 knockout mice. Immunofluorescence and freeze-fracture electron microscopy showed AQP4 protein expression in plasmalemma of fast-twitch skeletal muscle fibers of wild-type mice. Osmotic water permeability was measured in microdissected muscle fibers from the extensor digitorum longus (EDL) and fractionated membrane vesicles from EDL homogenates. With the use of spatial-filtering microscopy to measure osmotically induced volume changes in EDL fibers, half times ( t 1/2) for osmotic equilibration (7.5–8.5 s) were not affected by AQP4 deletion. Stopped-flow light-scattering measurements of osmotically induced volume changes in plasmalemma vesicles also showed no significant differences in water permeability. Similar water permeability, yet ∼90% decreased AQP4 protein expression was found in EDL from mdx mice that lack dystrophin. Skeletal muscle function was measured by force generation in isolated EDL, treadmill performance time, and in vivo muscle swelling in response to water intoxication. No differences were found in EDL force generation after electrical stimulation [42 ± 2 (wild-type) vs. 41 ± 2 (knockout) g/s], treadmill performance time (22 vs. 26 min; 29 m/min, 13° incline), or muscle swelling (2.8 vs. 2.9% increased water content at 90 min after intraperitoneal water infusion). Together these results provide evidence against a significant role of AQP4 in skeletal muscle physiology in mice.



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