polyphasic approach
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2021 ◽  
Vol 8 (1) ◽  
pp. 36
Author(s):  
Pei-Jie Han ◽  
Jian-Qiu Sun ◽  
Long Wang

In the survey of mycobiota of mudflats in China, two new sexually reproducing Talaromyces sect. Talaromyces species were discovered and studied using a polyphasic approach. These species are named here Talaromyces haitouensis (ex-type AS3.160101T) and Talaromyces zhenhaiensis (ex-type AS3.16102T). Morphologically, T. haitouensis is distinguished by moderate growth, green-yellow gymnothecia, orange-brown mycelium, and echinulate ellipsoidal ascospores. T. zhenhaiensis is characterized by fast growth, absence of sporulation, cream yellow to naphthalene yellow gymnothecia and mycelium, and smooth-walled ellipsoidal ascospores with one equatorial ridge. The two novelties are further confirmed by phylogenetic analyses based on either individual sequences of BenA, CaM, Rpb2, and ITS1-5.8S-ITS2 or the concatenated BenA-CaM-Rpb2 sequences.


2021 ◽  
Vol 54 (2) ◽  
pp. 81-102
Author(s):  
Ellen DellAgnolo ◽  
Márcio Da Silva Tamanaha ◽  
Charrid Resgalla Junior

The main transport vector for exotic-invasive species around the world is ballast water in merchant ships, crossing natural biogeographic barriers. This is one of the main factors responsible for the reduction and homogenization of the global biota. As phytoplankton is the main group transported by these vessels, this study assesses the risk of bioinvasion in the port complex of Itajaí and Navegantes (southern Brazil), using a polyphasic approach, mixing classical taxonomy with molecular biology. Ballast water collections were carried out to analyze the traditional taxonomy and for clonal cultivation in the laboratory. A successful cultivation of 12 local strains and 10-ballast water was successful. In the latter case, some fresh water, contrary to the statement by the commanders of the exchange of water in the deep ocean (> 200 m). Molecular identification was performed by sequencing the complete ITS region, confirming the presence of Thalassiosira minuscula Krasske, 1941, harmful and of public health interest, previously not mentioned for the region. The species Pleurosigma W. Smith, 1852; Asterionellopsis glacialis (Castracane) Round, 1990; Trieres mobiliensis (Bailey) Ashworth and E.C. Theriot, 2013; Thalassiosira minima Gaarder, 1951; Skeletonema pseudocostatum Medlin, 1991; Pectinodesmus holtmannii E. Hegewald, C. Bock and Krienitz, 2013; Neodesmus Hindák, 1976; and Pseudopediastrum boryanum (Turpin) E. Hegewald, 2005, were identified. The results indicate the possibility of growth of the species found in the ballast environment, which may negatively alter the disposal environment. Keywords: exotic species, molecular identification, marine phytoplankton, port complex of Itajaí and Navegantes.


Author(s):  
Peter Vandamme ◽  
Iain Sutcliffe

Chemotaxonomic methods played an important role in the development of the polyphasic approach to classification of Archaea and Bacteria. However, we here argue that routine application of these methods is unnecessary in an era when genomic data are available and sufficient for species delineation. Thus, authors who choose not to utilize such methods should not be forced to do so during the peer review and editorial handling of manuscripts describing novel species. Instead, we argue that chemotaxonomy will thrive if improved analytical methods are introduced and deployed, primarily by specialist laboratories, in studies at taxonomic levels above the characterisation of novel species.


Phycologia ◽  
2021 ◽  
pp. 1-16
Author(s):  
Melissa H. Pecundo ◽  
Fangfang Cai ◽  
Aimee Caye G. Chang ◽  
Hai Ren ◽  
Nan Li ◽  
...  

Fottea ◽  
2021 ◽  
Author(s):  
Fangfang Cai ◽  
Gongliang Yu ◽  
Yuchen Liu ◽  
Yuanquan Sun ◽  
Renhui Li

Phytotaxa ◽  
2021 ◽  
Vol 518 (1) ◽  
pp. 1-13
Author(s):  
MICHÉE A. APPADOO ◽  
DHANUSHKA N. WANASINGHE ◽  
YUSUFJON GAFFOROV ◽  
K.W. THILINI CHETHANA ◽  
AZIZ ABDURAZAKOV ◽  
...  

A survey of microfungi in the mountain areas of Uzbekistan revealed several specimens of cucurbitaria-like taxa. In this paper, one new species of Cucurbitaria is described based on a polyphasic approach, including morphological examination and molecular phylogeny. We compared the sexual morph (ascomata, asci, and ascospores) of the specimens collected with known species of Cucurbitaria and carried out multi loci (LSU, ITS, rpb2 and tef1-α) phylogenetic analyses. Cucurbitaria berberidicola sp. nov. is morphologically closely related to Cucurbitaria berberidis (the generic type) and C. oromediterranea. The sequence-based analyses verified a clear distinction, hence supporting the introduction of the new species.


2021 ◽  
Vol 9 (8) ◽  
pp. 724-734
Author(s):  
Vishal Dhundale ◽  
Vijayshree Hemke ◽  
Dhananjay Desai ◽  
Samruddhi Joshi ◽  
Pranali Shete ◽  
...  

Author(s):  
Ivo Sedláček ◽  
Pavla Holochová ◽  
Roman Sobotka ◽  
Hans-Jürgen Busse ◽  
Pavel Švec ◽  
...  
Keyword(s):  

Our polyphasic approach provides a new understanding of the taxonomy of novel pigmented Rugamonas species isolated from freshwater samples in Antarctica. The isolates showed considerable extracellular bactericidal secretions.


2021 ◽  
Vol 55 ◽  
pp. 102170
Author(s):  
K. Szubert ◽  
A. Toruńska-Sitarz ◽  
J. Stoń-Egiert ◽  
M. Wiglusz ◽  
H. Mazur-Marzec

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