volume electron microscopy
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Nature ◽  
2021 ◽  
Author(s):  
C. Shan Xu ◽  
Song Pang ◽  
Gleb Shtengel ◽  
Andreas Müller ◽  
Alex T. Ritter ◽  
...  

Nature ◽  
2021 ◽  
Author(s):  
C. Shan Xu ◽  
Song Pang ◽  
Gleb Shtengel ◽  
Andreas Müller ◽  
Alex T. Ritter ◽  
...  

Nature ◽  
2021 ◽  
Author(s):  
Larissa Heinrich ◽  
Davis Bennett ◽  
David Ackerman ◽  
Woohyun Park ◽  
John Bogovic ◽  
...  

EvoDevo ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
B. Duygu Özpolat ◽  
Nadine Randel ◽  
Elizabeth A. Williams ◽  
Luis Alberto Bezares-Calderón ◽  
Gabriele Andreatta ◽  
...  

AbstractThe Nereid Platynereis dumerilii (Audouin and Milne Edwards (Annales des Sciences Naturelles 1:195–269, 1833) is a marine annelid that belongs to the Nereididae, a family of errant polychaete worms. The Nereid shows a pelago-benthic life cycle: as a general characteristic for the superphylum of Lophotrochozoa/Spiralia, it has spirally cleaving embryos developing into swimming trochophore larvae. The larvae then metamorphose into benthic worms living in self-spun tubes on macroalgae. Platynereis is used as a model for genetics, regeneration, reproduction biology, development, evolution, chronobiology, neurobiology, ecology, ecotoxicology, and most recently also for connectomics and single-cell genomics. Research on the Nereid started with studies on eye development and spiralian embryogenesis in the nineteenth and early twentieth centuries. Transitioning into the molecular era, Platynereis research focused on posterior growth and regeneration, neuroendocrinology, circadian and lunar cycles, fertilization, and oocyte maturation. Other work covered segmentation, photoreceptors and other sensory cells, nephridia, and population dynamics. Most recently, the unique advantages of the Nereid young worm for whole-body volume electron microscopy and single-cell sequencing became apparent, enabling the tracing of all neurons in its rope-ladder-like central nervous system, and the construction of multimodal cellular atlases. Here, we provide an overview of current topics and methodologies for P. dumerilii, with the aim of stimulating further interest into our unique model and expanding the active and vibrant Platynereis community.


2021 ◽  
Author(s):  
Davis Laundon ◽  
Nathan Chrismas ◽  
Kimberley Bird ◽  
Seth Thomas ◽  
Thomas Mock ◽  
...  

The chytrids (phylum Chytridiomycota) are a major early-diverging fungal lineage of ecological and evolutionary importance. Despite their importance, many fundamental aspects of chytrid developmental and cell biology remain poorly understood. To address these knowledge gaps, we combined quantitative volume electron microscopy and comparative transcriptome profiling to create an "atlas" of the cellular and molecular basis of the chytrid life cycle, using the model chytrid Rhizoclosmatium globosum. From our developmental atlas, we show that zoospores exhibit a specialised biological repertoire dominated by inactive ribosome aggregates, and that lipid processing is complex and dynamic throughout the cell cycle. We demonstrate that the chytrid apophysis is a distinct subcellular structure characterised by high intracellular trafficking, providing evidence for division of labour in the chytrid cell plan, and show that zoosporogenesis includes "animal like" amoeboid cell morphologies resulting from endocytotic cargo transport from the interstitial maternal cytoplasm. Taken together, our results reveal insights into chytrid developmental biology and provide a basis for future investigations into early-diverging fungal cell biology.


eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Cesar Nava Gonzales ◽  
Quintyn McKaughan ◽  
Eric A Bushong ◽  
Kalyani Cauwenberghs ◽  
Renny Ng ◽  
...  

The biophysical properties of sensory neurons are influenced by their morphometric and morphological features, whose precise measurements require high-quality volume electron microscopy (EM). However, systematic surveys of nanoscale characteristics for identified neurons are scarce. Here, we characterize the morphology of Drosophila olfactory receptor neurons (ORNs) across the majority of genetically identified sensory hairs. By analyzing serial block-face electron microscopy (SBEM) images of cryofixed antennal tissues, we compile an extensive morphometric dataset based on 122 reconstructed 3D models for 33 of the 40 identified antennal ORN types. Additionally, we observe multiple novel features - including extracellular vacuoles within sensillum lumen, intricate dendritic branching, mitochondria enrichment in select ORNs, novel sensillum types, and empty sensilla containing no neurons - which raise new questions pertinent to cell biology and sensory neurobiology. Our systematic survey is critical for future investigations into how the size and shape of sensory neurons influence their responses, sensitivity and circuit function.


2021 ◽  
Vol 27 (S1) ◽  
pp. 1136-1137
Author(s):  
Chandran Sabanayagam ◽  
Wayne Treible ◽  
Jean Ross ◽  
Jeffrey Caplan

2021 ◽  
Vol 27 (S1) ◽  
pp. 776-777
Author(s):  
Yu Yuan ◽  
Sabrina Clusiau ◽  
Raynald Gauvin ◽  
Christopher Bleck ◽  
Adrian Phoulady ◽  
...  

2021 ◽  
Vol 220 (9) ◽  
Author(s):  
Paolo Ronchi ◽  
Giulia Mizzon ◽  
Pedro Machado ◽  
Edoardo D’Imprima ◽  
Benedikt T. Best ◽  
...  

Cells are 3D objects. Therefore, volume EM (vEM) is often crucial for correct interpretation of ultrastructural data. Today, scanning EM (SEM) methods such as focused ion beam (FIB)–SEM are frequently used for vEM analyses. While they allow automated data acquisition, precise targeting of volumes of interest within a large sample remains challenging. Here, we provide a workflow to target FIB-SEM acquisition of fluorescently labeled cells or subcellular structures with micrometer precision. The strategy relies on fluorescence preservation during sample preparation and targeted trimming guided by confocal maps of the fluorescence signal in the resin block. Laser branding is used to create landmarks on the block surface to position the FIB-SEM acquisition. Using this method, we acquired volumes of specific single cells within large tissues such as 3D cultures of mouse mammary gland organoids, tracheal terminal cells in Drosophila melanogaster larvae, and ovarian follicular cells in adult Drosophila, discovering ultrastructural details that could not be appreciated before.


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