Different Approaches to Assessing Pollution Load: The Case of Nitrogen-Related Grey Water Footprint of Barley and Soybean in Argentina

Agriculture is among the main causes of water pollution. Currently, 75% of global anthropogenic nitrogen (N) loads come from leaching/runoff from cropland. The grey water footprint (GWF) is an indicator of water resource pollution, which allows for the evaluation and monitoring of pollutant loads (L) that can affect water. However, in the literature, there are different approaches to estimating L and thus contrasting GWF estimates: (A1) leaching/runoff fraction approach, (A2) surplus approach and (A3) soil nitrogen balance approach. This study compares these approaches for the first time to assess which one is best adapted to real crop production conditions and optimises GWF calculation. The three approaches are applied to assess N-related GWF in barley and soybean. For barley in 2019, A3 estimated a GWF value 285 to 196% higher than A1, while in 2020, the A3 estimate was 135 to 81% higher. Soybean did not produce a GWF due to the crop characteristics. A3 incorporated N partitioning within the agroecosystem and considered different N inputs beyond fertilization, improving the accuracy of L and GWF estimation. Providing robust GWF results to decision-makers may help to prevent or reduce the impacts of activities that threaten the world’s water ecosystems and supply.

Legume-Rhizobium Strain Specificity Enhances Nutrition and Nitrogen Fixation in Faba Bean (Vicia faba L.)

This study reports the effectiveness of some selected rhizobium strains in enhancing nitrogen fixation and nutrient uptake in Vicia faba L. Multi-location field experiments were conducted for two years (2016 and 2017) using a split-plot in randomized complete block design. Treatments comprised six rhizobium strains as the main plot factor and three varieties of Vicia faba as the sub-plot factor. Non-inoculated plants with or without N fertilizer served as +N and −N controls, respectively. Peat carrier-based inoculant of each strain was applied at the rate of 10 g kg−1 seed. Data on nodulation were taken at the late-flowering stage, whereas nitrogen and phosphorus concentrations in plant parts were analyzed at physiological maturity. The total nitrogen difference method was employed to quantify nitrogen fixation. Location x rhizobium strain x variety interaction had a significant effect on nodule dry weight plant−1. Rhizobium strains significantly enhanced nodulation, nitrogen fixation, nutrient uptake and soil nitrogen balance. Inoculation with NSFBR-12 and NSFBR-15 resulted in the highest nitrogen fixed, nutrient uptake and soil nitrogen balance. Vicia faba inoculated with the two top performing strains, NSFBR-12 and NSFBR-15 fixed respectively 87.7% and 85.5% of the total nitrogen uptake. Non-inoculated plants fulfilled proportionately more of the total nitrogen uptake through nitrogen derived from the soil rather than fixed nitrogen. Soil available phosphorus and pH had appreciable influences on nitrogen and phosphorus uptake of inoculated Vicia faba. Inoculation with competitive and effective rhizobium strains can improve soil nitrogen balance, nitrogen fixation and nutrient uptake of Vicia faba.