Combined Butyric Acid and Methane Production from Grass Silage in a Novel Green Biorefinery Concept

In a Green Biorefinery, grass silage can be a source for lactic acid, proteins, amino acids and fibres. Processing residues can be used for anaerobic digestion and methane production. But by changing the ensiling conditions, butyric acid fermentation can be achieved. That makes grass silage also a potential substrate for a combined butyric acid and methane production. The objective of this study was to determine the potential of butyric acid production at different ensiling conditions applied to grass and measuring the methane yield potential of solid residues after a separation step. The highest butyric acid concentration in the produced press juice was 20.1 ± 4.5 g kg−1 and was achieved by carbonated lime addition and a reduced dry matter content after 90 days at mesophilic storage conditions. This resulted in a theoretical butyric acid yield of 332 kg ha−1 a−1. For the fibrous leftover press cake, a theoretical methane production potential of 2778 m3CH4 ha−1 a−1 was reached. The results show that theoretically a combined production of butyric acid and methane can be realised in a Green Biorefinery concept. Graphic Abstract

Production of Green Biorefinery Protein Concentrate Derived from Perennial Ryegrass as an Alternative Feed for Pigs

Perennial rye grass is a widely used forage species in Ireland, on which the ruminant sector of agriculture is heavily dependent. While this species of grass is the primary source of fodder for cows, it is also abundant in plant protein, which could form a potential alternative ingredient in monogastric animal feed using a green biorefinery approach. In this study, perennial rye grass was processed using a novel biorefining process to extract value added products including protein as a potential replacement for soybean meal in monogastric feeds. Feed trials were conducted on a commercial farm with 55 weaner pigs for 31 days until slaughter. The diets comprised a control and a trial diet which integrated the green biorefinery protein concentrate. The effects of the new diet were determined by measuring the daily feed intake (DFI), average weight gain (AWG) and feed conversion ratio (FCR). Amino acid profiles of grass protein concentrate and soybean meal were comparable, with the latter having a slightly higher amount of total protein content, lysine and cysteine. The DFI and ADW indicated that the treatment diet was superior to the control. DFI for the treatment diet (1.512 kg/d) was 8% higher than the control diet (1.400 kg/d) by the end of the trial. Additionally, the ADW for the treatment diet was 6.44% higher than that achieved in the control sample. Meanwhile, FCR calculations indicated that the treatment diet is just as efficient as the conventional diet. Overall, the results of the study indicate positive potential for perennial ryegrass-derived green biorefinery protein concentrate as an alternative protein source for pig feed formulations in Ireland.