Our ability to briefly retain information is often limited. Proactive Interference (PI) might contribute to these limitations (e.g., when it is hard to reject items in a recognition test that have appeared recently). In visual Working Memory (WM), spatial information might protect WM against PI, especially if encoding items together with their spatial locations makes item-location combinations less confusable than simple items without a spatial component. Here, I ask (1) if PI is observed for spatially distributed items, (2) if it arises among simple items or among item-location combinations, and (3) if spatial information affects PI at all. I show that PI is reliably observed for spatially distributed items except when it is weak. PI mostly reflects items that appear recently or frequently as memory items, while occurrences as test items play a smaller role, presumably because their temporal context is easier to encode. Through mathematical modeling, I then show that interference occurs among simple items rather than item-location combinations. Finally, to understand the effects of spatial information, I separate the effects of (a) the presence and (b) the predictiveness of spatial information on memory and its susceptibility to PI. Memory is impaired when items are spatially distributed, but, depending on the analysis, unaffected by the predictiveness of spatial information. In contrast, the susceptibility to PI is unaffected by either manipulation. Visual memory is thus impaired by PI for spatially distributed items due to interference from recent memory items (rather than test items or item-location combinations).