Luminescence measurements: reference materials for composition of sodium adenosine triphosphate aqueous solution

The authors evaluated the possibility of using the luminometry method which allows checking the level of sanitation and hygiene in industries and is one of the most effective methods of «rapid microbiology». Such key stages of development of reference material for sodium adenosine triphosphate with the certified values as analysis of similar reference materials, selection of source material of the reference material, conducting experimental studies, and establishing the metrological characteristics of reference material with traceability to state primary measurement standard were provided in the paper. Considering that the selective optical density at the characteristic wavelength is directly proportional to the concentration of the absorbing component (sodium adenosine triphosphate), the stability of R M was studied by spectrophotometry. The stability of the RM material to assess the uncertainty of the measurement results was carried out by the isochronous method. The result of the study is the possibility of ensuring the traceability of measurement results based on luminescence to primary measurement standard by sodium adenosine triphosphate concentration. In addition to the above, the authors selected the optimal value of that concentration and established metrological properties for GSO 11606-2020 R M for the composition of sodium adenosine triphosphate aqueous solution (mass concentration: from 0,09 to 0,11 g/dm3; extended uncertainty: 0,015 g/dm3). Usage of this CRM in performing tests, calibrations, and verification of measuring instruments allows providing the unbiased assessment of the measuring capabilities of luminescent measuring instruments, including in terms of determining the error of the measurements carried out.

Calibration platform controller of the laser Doppler anemometer

Primary standards for the unit of air flow velocity often use a Laser Doppler Anemometer (LDA) as the primary measurement standard. A rotating disc with a fixed diameter is used to calibrate LDA. The paper proposes calibration platform based on the precision mechanics of HDD disk. A disk rotation controller has been developed for the platform. Deviations of the disk rotation speed do not exceed 0.01% RMS in the range of angular speeds of 600-4800 rpm.

The new certified reference material of chlorobenzene as a means of providing the metrological traceability for calibration and measurements

The article is devoted to the development of a reference material of chlorobenzene composition, providing metrological traceability of measurement results to the State primary measurement standard of units of mass (molar) fraction and mass (molar) concentration of organic components in liquid and solid substances and materials based on liquid and gas chromatography-mass spectrometry with isotopic dilution and gravimetry GET 208-2014.The paper considers an indirect method for determining the purity of the starting substance, describes the main stages of development of the reference material, and presents the results of the evaluation of the reference material metrological characteristics, including studies on homogeneity and stability.The reference material of the composition of chlorobenzene has the following metrological characteristics: mass fraction of chlorobenzene (from 993.0 to 999.8 mg/g); molar fraction of chlorobenzene (from 99.30% to 99.98%); the expanded uncertainty of the certified characteristics is 0.02% (at k = 2).The reference material ensures metrological traceability to units of measuring instruments and measurement results, and is intended for verification, and calibration and graduations.

State primary measurement standard of ultrasound power unit in water GET 169-2019

Results of works on improvement of the state primary special measurement standard of ultrasound power unit in water GET 169-2005 are given. As a result of the inclusion of two new reference ultrasound power meters in the standard, the frequency and dynamic ranges of reproduction and transmission of the power unit to lower-level measuring instruments were expanded using absorbing targets for measuring the radiation effect of the ultrasonic wave and the absorbed ultrasonic energy. That is very important for metrological support of modern high-tech medical ultrasound equipment, including focused high-intensity ultrasound beams for the treatment of cancer. The automation of the measurement process used in the updated standard has reduced significantly the complexity of verification procedures increasing the accuracy of measurements.

Multiparametric reference materials for metrological support in the area of coating parameter control

The present work analyses the state and development prospects of metrological support and standardization of in the field of measuring surface density, thickness and chemical composition of coatings. The main characteristics of the State Primary Measurement Standard GET 168-2015 and developed multiparameter reference materials are described.