Isopropanol DNA Precipitation - Best for DNA Concentration v1

Protocol to precipitate extracted DNA from an aqueous solution to increase concentration or resolubilize in a different storage buffer. *Use isopropanol DNA precipitation if your DNA is suspended in a very large volume, if your DNA concentration is low, or you are trying to concentrate large molecular weight DNA fragments and remove smaller fragments. *Use ethanol DNA precipitation if you are trying to remove salt contamination or precipitate small DNA fragments. Additional Resources: New England Biolabs, "DNA Precipitation: Ethanol vs. Isopropanol". June 23, 2015 Green, Michael R. and Joseph Sambrook, "Precipitation of DNA with Isopropanol". doi:10.1101/pdb.prot093385Cold Spring Harb Protoc2017. Qiagen, "How can I precipitate genomic DNA using isopropanol?".

Evaluation of a high-performance storage buffer with 3D XPoint devices for the DUNE data acquisition system

The DUNE detector is a neutrino physics experiment that is expected to take data starting from 2028. The data acquisition (DAQ) system of the experiment is designed to sustain several TB/s of incoming data which will be temporarily buffered while being processed by a software based data selection system. In DUNE, some rare physics processes (e.g. Supernovae Burst events) require storing the full complement of data produced over 1-2 minute window. These are recognised by the data selection system which fires a specific trigger decision. Upon reception of this decision data are moved from the temporary buffers to local, high performance, persistent storage devices. In this paper we characterize the performance of novel 3DXPoint SSD devices under different workloads suitable for high-performance storage applications. We then illustrate how such devices may be applied to the DUNE use-case: to store, upon a specific signal, 100 seconds of incoming data at 1.5 TB/s distributed among 150 identical units each operating at approximately 10GB/s.

A Switched-Reluctance Motor Drive Powered by Switch-Mode Rectifier with Battery Energy Storage Buffer

This paper presents a three-phase full-bridge boost switch-mode rectifier (SMR) powered switched-reluctance motor (SRM) drive with battery energy storage buffer. It covers the designing of the power circuits and control schemes for the two power stages. Except for having a superior line-drawn power quality, the boost-able DC-link voltage of the SRM drive can enhance the SRM driving performance in a wide speed range and sent back the recovered regenerative braking energy to the grid successfully. The next is establishing a battery energy storage system (BESS) with a bidirectional interface DC-DC converter connected to the motor drive DC-link for providing an energy buffer. The proposed parallel operation strategy has three possible inter-connected operations. (i) Grid-to-battery (G2B) charging: The battery charged by the grid with a good line drawn power quality. (ii) B2G discharging operation: The battery sending power back to the grid by the interface converter and the SMR. (iii) Battery buffer operation: Powering of the SRM drive simultaneously by the utility grid and the battery with the proposed parallel operation strategy.

Comparison of media and standards for SARS-CoV-2 RT-qPCR without prior RNA preparation

Since the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, there have been demands on the testing infrastructure that have strained testing capacity. As a simplification of method, we confirm the efficacy of RNA extraction-free RT-qPCR and saline as an alternative patient sample storage buffer. In addition, amongst potential reagent shortages, it has sometimes been difficult to obtain inactivated viral particles. We have therefore also characterized armored SARS-CoV-2 RNA from Asuragen as an alternative diagnostic standard to ATCC genomic SARS-CoV-2 RNA and heat inactivated virions and provide guidelines for its use in RT-qPCR.

Functionalized Carbonaceous Materials as Cathode for Lithium-Ion Batteries

ABSTRACTActivated carbon materials are integrated into functionalization of graphene nano-sheets to serve as a high-power lithium cathode. The electrochemical performance shows that the composite displays the highest reversible capacity (c. 170 mAh g-1) comparing with functionalized graphene and activated carbon. Also, approximately 92% of its capacity can be retained after 4,000 cycles at a current of 1 A g-1. Moreover, the composite exhibits an excellent rate performance, a reversible capacity of 90 mAh g-1 even at 6 A g-1, which corresponds to the power density of 15.2 kW kg-1 and energy density of 227 Wh kg-1, respectively. The high performance of this composite can be attributed to the fact that the activated carbon particles not only reduce the graphene sheet stacking thus making it easier for ions to diffuse, but also act as an ion storage buffer against accelerating electron transfer.