Differential Responses of Soil Bacterial and Fungal Community to Short-Term Crop Tree Management in a Larix gmelinii Plantation

Crop tree management (CTM) is a widely applicable silviculture technology that is used to improve the performance of individual trees. However, only little information is available about the effects of the CTM regime on the soil microbial community structure. We conducted a study to explore the effects of short-term (five years) CTM on the soil bacterial and fungal diversity, community composition, and structure in the 0–10 cm soil layer in a Larix gmelinii (Rupr.) Kuzen. plantation. We set out to investigate the differential response of bacterial and fungal communities to variations in soil properties mediated by short-term CTM. Compared with the control plots, the soil microbial biomass carbon and microbial biomass nitrogen in CTM increased significantly by 64.2% and 32.3%, respectively. CTM significantly promoted the content of soil organic carbon, dissolved organic carbon, and nitrate nitrogen, and reduced the content of dissolved organic nitrogen. CTM changed the Shannon and Simpson indices of soil fungi to a remarkable extent but had little effect on the α diversity of bacterial communities. The bacterial β diversity was more sensitive to CTM than fungi. The relative abundance of Verrucomicrobiae (the dominant class of soil bacteria) in CTM was significantly increased by 78.2%, while the relative abundance of Agaricomycetes (dominant class for soil fungi) was reduced by 43.3%. We observed a significantly increased number of unique OTUs for soil fungi in the CTM plots. Redundancy analysis showed that dissolved organic carbon, soil moisture, and total phosphorus content significantly affected the composition of bacterial communities, while soil dissolved organic nitrogen, C/N, and total phosphorus drove the high variation in fungal community composition. Overall, our results emphasize the divergent response of soil bacterial and fungal communities in Larix gmelinii plantations to short-term CTM. We must pay more attention to the functional role of soil microbiota in future forest management.

Embryogenic callus induction from immature zygotic embryos and genetic transformation of Larix kaempferi 3x Larix gmelinii 9

To date, there are few reports of the successful genetic transformation of larch and other conifers, mainly because it is difficult to transform and integrate exogenous genes. In this study, hybrid larch Larix kaempferi 3x Larix gmelinii 9 cones were collected on June 27, July 1, July 4, July 7 and July 16, 2017. Embryogenic callus induction was studied using a combination of different plant growth regulators and concentrations. The results showed that July 1 was the best stage; the highest induction rate was 10.83%, which cultured in BM medium (Button medium, which formula was listed in S1 Table) with 1.0 mg/L 2,4-D (2,4-dichlorophenoxyacetic acid) and 0.2 mg/L KT(kinetin). When cultured on a proliferation medium for 12 days, proliferation was the fastest, reaching 323.08%, which could also maintain the freshness and vitality. The suitable pre-culture medium for somatic embryogenesis was 1/4 BM medium containing 10 g/L inositol and 60 g/L sucrose. The combination of 45 mg/L ABA (abscisic acid) and 75 g/L PEG4000 (Polyethyene glycol 4000) could promote the number of somatic embryos, and reached the maximum, 210 140 per 1 g FW. The genetic transformation was carried out by the Agrobacterium-mediated transformation method with embryogenic callus cultured for 12 days. The results showed the optimal OD600 of the infection solution(suspension of A. tumefaciens) was 0.5, co-culture time was 2 days, and screening concentration of Hyg (hygromycin B) was 4 mg/L. In this study, the transformation rate of resistance callus was 32.1%. It provides a reference for low genetic transformation efficiency of larch at present. This study could be beneficial for the innovation and breeding of larch by genetic engineering and provides a certain basis for rapid propagation of excellent larch germplasm resources and genetic engineering breeding of larch and other conifers.

Propagation velocity model and two-dimensional defect imaging of stress wave in larch (Larix gmelinii) wood

The propagation law of stress wave in larch (Larix gmelinii) wood was studied in this work. External factors affecting the propagation velocity of stress wave in wood cross-section were studied using the orthogonal experiment method. The most influential factors were shown by the experimental results, and the parameters of the propagation velocity model of stress wave in larch wood were optimized. Based on the optimized propagation velocity model, combined with the traditional defect determination method, a twelve-directional stack imaging (TDSI) steps system was developed for larch wood internal defect detection. The analysis results showed that of the three external factors of temperature, moisture content, and illumination duration, temperature had the greatest influence on the propagation velocity model of stress wave in larch wood cross-section. Using TDSI to image the defective larch wood not only can locate the defective area, but also it can achieve a high imaging precision of 95.52%, and the imaging precision is unrelated to the location of the defect, which has a good quantitative defect detection effect.

Phytolith Content In Larix Gmelinii Forest Soils And Its Relations With Soil Properties

Aims Phytolith occluded carbon (PhytOC) has become one of the important mechanisms of long-term carbon sequestration in soil. Ecosystems with higher latitude in the northern hemisphere are expected to face the largest loss of soil carbon due to global warming. The contributions of phytoliths storage in soil layers of cold temperate forest need to be studied in depth. Methods We examined soil phytolith contents and other soil physicochemical properties of Larix gmelinii forest soils in Daxing’anling. ANOVA was employed to analyze differences in the quantitative characteristics of phytoliths. Bivariate correlation, regression analysis, principal component analysis and redundancy analysis were employed to analyze the relations between soil phytolith and soil properties. Results The phytolith in Larix gmelinii forest soil was mainly in elongate shape with a mean content of 19.1 g kg− 1. The phytolith had a stronger correlation with phosphorus rather than potassium and nitrogen and had no correlation with soil water content, and the dominant factors were Na, Mg and K content. Soil phytolith storage in the cold temperate zone (41.0 t ha− 1) was significantly higher than that in the tropical and subtropical zone. Conclusions Phytolith is an important way for long-term sequestration of soil organic carbon. Soil phytolith storage is affected by soil physicochemical properties and climate. However, the acid-base level of soil is more important for phytolith preservation. The effects of hydrothermal conditions on the yield and stability of phytoliths on a large spatial scale need to be studied in future.