Genetic polymorphism of local Abkhazian grape cultivars

Local grape cultivars from different countries of the world are an important part of the gene pool of this culture. Of particular interest are the genotypes of the most ancient regions of viticulture. The territories of the subtropical zone of Georgia and the central part of Abkhazia belong to one of the centers of origin of the cultural grapevine. The purpose of the work was to genotype native Abkhazian grape cultivars, to study their genetic diversity based on DNA profiling data and to compare them with the genotypes of local varieties of other viticultural regions. Samples of plants were taken on the territory of the Republic of Abkhazia in private farmsteads and in the collection of the agricultural firm “Vina i Vody Abkhazii“ (“Wines and Waters of Abkhazia”). The genotyping of the Abkhazian cultivars Avasirhva, Agbizh, Azhapsh, Azhizhkvakva, Azhikvaca, Atvizh, Atyrkuazh, Achkykazh, Kachich was carried out using 14 DNA markers, 9 of which are standard microsatellite markers recommended for the identification of grape varieties. To improve our knowledge about the sizes of the identified alleles, we used the DNA of grape cultivars with a known allelic composition at the analyzed loci. Statistical analysis of the data showed that the observed heterozygosity for the analyzed loci exceeded expected values, which indicates a genetic polymorphism of the studied sample of varieties. Evaluation of genetic similarity within the analyzed group based on the results of genotyping at 14 loci showed that the cultivars Kachich and Azhapsh differed from the other Abkhazian varieties. The obtained DNA profiles of the Abkhazian cultivars were checked for compliance with DNA-fingerprints of grape varieties in the Vitis International Variety Catalogue. The Georgian varieties Azhizhkvakva and Tsitska turned out to be synonyms according to DNA profiles, two varieties from the Database (Italian Albana bianca and Georgian Ojaleshi) have differences in DNA-fingerprints from the varieties Atyrkuazh and Azhikvatsa only in one allele, respectively. When comparing the identified Abkhazian grape genotypes, their difference from the sample of Dagestan, Don, Greek, Turkish, Italian, Spanish, and French varieties and genetic similarity with the genotypes of Georgian grapes were shown.

The synergy of patterns vs. processes at a community level: a successful mechanism underlying subtropical native forests along the urban riparian zone

Riparian zone possesses ecological position with biota differing from aquatic body and terrestrial lands, and plant-animal coevolution may be the main factor for the framework of riparian vegetation. In the current study, the riparian plant community patterns along the subtropical mountainous riparian belts of Chongqing, China, was proposed to be regulated by co-evolving with the avifauna through propagule-dispersal process. The results show that: 1) the forests’ species composition and vertical layers are dominated by native catkins of Moraceae species with adapting traits of small and numerous propagules to frugivorous bird species, revealing an evolutionary trend different from the one in the terrestrial plant climax communities in the subtropics, and which forms a biological base for the plant-bird co-evolution; 2) there are significant associations of plant-bird species clusters, i.e., four plant-bird co-evolution groups (PBs) were divided out according to the plant species’ dominance and growth form relating to the fruit-dispersing birds’ abundance; 3) the correlation intensity within PB ranks as PBⅠ>Ⅱ>Ⅳ>Ⅲ, indicating the PBⅠis the leading type of co-evolution mainly shaped by the dominant plant species; 4) the PB correlation may be a key node between patterns vs. process of a riparian ecosystem responsible for the native vegetation, or even the ecosystem health. The results theoretically contribute new evidence to plant-animal co-evolution interpreting the forests’ characters in riparian environments, and urban planner and managers may simulate the native forests for restoring a more stable riparian biota, a better functioning ecosystem in subtropical zone.

Changes in the physiological state of peach plants influenced by drought

The article presents experimental data on physiological and biochemical studies of stone fruit culture on the example of Persica vulgaris (Mill.) In the humid subtropics of Russia. The research has been carried out since 2011 in the Laboratory of plant physiology and biochemistry of the Subtropical Scientific Center of the Russian Academy of Sciences on peach varieties of different ripening periods grown in the humid subtropics of Russia. To identify any physiological changes in the body of peach plants during drought, especially in summer, comprehensive diagnostic studies of the water regime (leaf turgidity, determination of the thickness of the leaf blade, water deficiency) and pigment composition are carried out, characterizing the functional state of peach plants. Under unfavorable conditions, promising varieties have a higher ratio of chlorophylls to carotenoids, which confirms their more developed adaptive potential. Studies of this nature have been carried out in order to identify and select resistant peach plants among the studied varieties, which would correspond to the humid region of the Black Sea coast of the Krasnodar Territory. In the subtropical zone of our region, the issue of drought resistance has always been acute, since the impact of negative environmental factors (cool spring, accompanied by drizzling rains and a long summer shortage of air and soil moisture) on peach plants lead to a decrease in productivity and a deterioration in the quality of fruits, which makes their unsuitable and non-transportable. It has been found that among the tested plants, peach varieties Maycrest, Fayet, Larisa have been distinguished by high physiological indicators, such as: low water deficiency – 12,20–14,65%, leaf turgidity ­– 0,18 mm, saturation of leaf tissues within 70,12–79,21%, the content of carotenoids is 0,5–0,7 units, which is a confirming sign of their high resistance to the negative effects of weather conditions in the south of Russia.

Extending the Cultivation Area of Pecan (Carya illinoinensis) Toward the South in Southeastern Subtropical China May Cause Increased Cold Damage

Pecan (Carya illinoinensis) is an important nut tree species in its native areas in temperate and subtropical North America, and as an introduced crop in subtropical southeastern China as well. We used process-based modeling to assess the effects of climatic warming in southeastern China on the leaf-out phenology of pecan seedlings and the subsequent risk of “false springs,” i.e., damage caused by low temperatures occurring as a result of prematurely leafing out. In order to maximize the biological realism of the model used in scenario simulations, we developed the model on the basis of experiments explicitly designed for determining the responses modeled. The model showed reasonable internal accuracy when calibrated against leaf-out observations in a whole-tree chamber (WTC) experiment with nine different natural-like fluctuating temperature treatments. The model was used to project the timing of leaf-out in the period 2022–2099 under the warming scenarios RCP4.5 and RCP8.5 in southeastern China. Two locations in the main pecan cultivation area in the northern subtropical zone and one location south of the main cultivation area were addressed. Generally, an advancing trend of leaf-out was projected for all the three locations under both warming scenarios, but in the southern location, a delay was projected under RCP8.5 in many years during the first decades of the 21st century. In the two northern locations, cold damage caused by false springs was projected to occur once in 15–26 years at most, suggesting that pecan cultivation can be continued relatively safely in these two locations. Paradoxically, more frequent cold damage was projected for the southern location than for the two northern locations. The results for the southern location also differed from those for the northern locations in that more frequent cold damage was projected under the RCP4.5 warming scenario (once in 6 years) than under the RCP8.5 scenario (once in 11 years) in the southern location. Due to the uncertainties of the model applied, our conclusions need to be re-examined in an additional experimental study and further model development based on it; but on the basis of our present results, we do not recommend starting large-scale pecan cultivation in locations south of the present main pecan cultivation area in southeastern subtropical China.

Asymmetric Differences in the Effects of Average Air Temperature and Solar Radiation on Early Rice and Late Rice Yield

China is the world’s largest rice producer. Thus, the stability of rice production plays a decisive role in food security. Among the types of rice, double rice (including early rice and late rice) accounts for the largest proportion of rice in China. Climate change is widely expected to affect rice yields. Studying the response of double rice yield to climate change will benefit strategic decisions related to future crop adaptation. In this paper, the relationship between climate factors and the yield of double rice during 1992–2013 in south China was analysed to determine the responses of double rice yield to climate change. The results showed that the daily average air temperature during the early rice and late rice growing seasons increased by 0.34 °C and 0.68 °C, 0.29 °C and 0.67 °C, and 0.11 °C and 0.31 °C per 10-year period in the northern subtropical zone (NST), middle subtropical zone (MST) and south subtropical zone (SST), respectively, in the last 20 years. The change trend in solar radiation was not obvious, but it fluctuated greatly. A 1 °C increase in average air temperatures decreased early rice yield by 5.36% and 2.16% in SST and MST, respectively; decreased late rice yield by 0.75% and 1.43% in MST and NST, respectively; and increased late rice yield by 3.93% in SST. A solar radiation increases of 100 MJ m−2 increased early rice yield by 1.02%, 1.54% and 1.71% in SST, MST and NST, respectively, and decreased late rice yield by 0.89% in SST. We found that annual average temperatures of 17.3 °C and 18.6 °C were the early rice and late rice yield variation thresholds, respectively; in addition, above the background temperature in south China, the early rice yield will decrease and the late rice yield will increase.

Characterizing Spatial Patterns of Pine Wood Nematode Outbreaks in Subtropical Zone in China

Pine wood nematode (PWN), Bursaphelenchus xyophilus, originating from North America, has caused great ecological and economic hazards to pine trees worldwide, especially affecting the coniferous forests and mixed forests of masson pine in subtropical regions of China. In order to prevent PWN disease expansion, the risk level and susceptivity of PWN outbreaks need to be predicted in advance. For this purpose, we established a prediction model to estimate the susceptibility and risk level of PWN with vegetation condition variables, anthropogenic activity variables, and topographic feature variables across a large-scale district. The study was conducted in Dangyang City, Hubei Province in China, which was located in a subtropical zone. Based on the location of PWN points derived from airborne imagery and ground survey in 2018, the predictor variables were conducted with remote sensing and geographical information system (GIS) data, which contained vegetation indices including normalized difference vegetation index (NDVI), normalized difference moisture index (NDMI), normalized burn ratio (NBR), and normalized red edge index (NDRE) from Sentinel-2 imagery in the previous year (2107), the distance to different level roads which indicated anthropogenic activity, topographic variables in including elevation, slope, and aspect. We compared the fitting effects of different machine learning algorithms such as random forest (RF), K-neighborhood (KNN), support vector machines (SVM), and artificial neural networks (ANN) and predicted the probability of the presence of PWN disease in the region. In addition, we classified PWN points to different risk levels based on the density distribution of PWN sites and built a PWN risk level model to predict the risk levels of PWN outbreaks in the region. The results showed that: (1) the best model for the predictive probability of PWN presence is the RF classification algorithm. For the presence prediction of the dead trees caused by PWN, the detection rate (DR) was 96.42%, the false alarm rate (FAR) was 27.65%, the false detection rate (FDR) was 4.16%, and the area under the receiver operating characteristic curve (AUC) was equal to 0.96; (2) anthropogenic activity variables had the greatest effect on PWN occurrence, while the effects of slope and aspect were relatively weak, and the maximum, minimum, and median values of remote sensing indices were more correlated with PWN occurrence; (3) modeling analysis of different risk levels of PWN outbreak indicated that high-risk level areas were the easiest to monitor and identify, while lower incidence areas were identified with relatively low accuracy. The overall accuracy of the risk level of the PWN outbreak was identified with an AUC value of 0.94. From the research findings, remote sensing data combined with GIS data can accurately predict the probability distribution of the occurrence of PWN disease. The accuracy of identification of high-risk areas is higher than other risk levels, and the results of the study may improve control of PWN disease spread.

Phytoconstituents and Bioactivity of Plants of the Genus Spiraea L. (Rosaceae): A Review

The genus Spiraea L. belongs to the Rosaceae Juss. family and includes more than 100 species distributed in the temperate zone and subtropical zone of the Northern Hemisphere at the center of species diversity in East Asia. Representatives of the genus are known as ornamental plants with many forms and varieties, are widely used in conventional medicine, and have a high resource potential. This review provides information on the diversity of phenolic compounds (flavonoids, phenolcarboxylic acids, and lignans), terpenoids, alkaloids, steroids, and other classes of secondary metabolites in the species of Spiraea worldwide. The article also presents little-known and hard-to-find data published in Russian concerning Spiraea phytochemistry. The biological activities of extracts and their fractions and of individual compounds having different types of biological activity (e.g., antioxidant, antibacterial, anti-inflammatory, and antifungal) are discussed. Data about biotechnological research on representatives of the genus Spiraea are presented too. The analysis of the literature showed that further chemical and pharmacological studies on Spiraea plants are quite promising.

Spatio-Temporal Variation Analysis of the Biological Boundary Temperature Index Based on Accumulated Temperature: A Case Study of the Yangtze River Basin

Active accumulated temperature is an important index of agricultural heat resources in a region. Based on the temperature data of the Yangtze River Basin from 1970 to 2014, this paper analyzed the characteristics of the temporal and spatial variations of the biological boundary temperature in the Yangtze River Basin. The main conclusions were drawn as follows: (1) since 1970, the accumulated temperature of ≥0 °C in the northern subtropical zone, mid-subtropical zone, and plateau climate zone showed overall increasing trends, and the trends were 122 (p < 0.001), 87.7 (p < 0.001), and 75.3 °C/10a (p < 0.001), respectively. The accumulated temperature of ≥5 °C showed an upward trend, and the change tendency rates were 122.6 (p < 0.001), 90.5 (p < 0.001), and 81.4 °C/10a (p < 0.001), respectively. The accumulated temperature of ≥10 °C showed overall increasing trends and the trends were 115.7 (p < 0.001), 92.5 (p < 0.001), and 78.9 °C/10a (p < 0.001). Accumulated temperatures of ≥0 °C, ≥5 °C, and ≥10 °C in the northern subtropical zone increased significantly higher than that in the mid-subtropical zone and plateau climate zone. (2) The accumulated temperatures of ≥0 °C, ≥5 °C, and ≥10 °C in the northern subtropical zone showed an abrupt change in 1997. In the mid-subtropical zone and plateau climate zone, there was an abrupt change in the accumulated temperatures of ≥0 °C and ≥5 °C in 1994, and in the northern subtropical zone, the abrupt change of the accumulated temperature ≥10 °C occurred in 1998. (3) There are obvious differences in the biological boundary temperature within the Yangtze River Basin, and the stations with large increases are mainly distributed in the middle and lower reaches, such as the Hanshui Basin, the Poyang Lake Basin, the Taihu Lake Basin, and the middle and lower reaches of the mainstream area. The initial day, final day, and continuous days showed a trend of advancement, postponement, and extension, respectively. Besides, the heat resources showed significant increasing trends, which is of guiding significance for the future production and development of agriculture in the region. With the increase of heat resources in the Yangtze River Basin, appropriate late-maturing varieties should be selected in variety breeding, to make full use of heat resources and improve the quality of agricultural products. Secondly, the planting system should be adjusted and the multiple cropping index improved to steadily increase agricultural output. This brings new opportunities to adjust the structure of the agricultural industry and increase farmers’ income, in the Yangtze River basin.

A REVIEW ON EPIDEMIOLOGY, PATHOGENESIS AND TREATMENT OF LEPTOSPIROSIS

Leptospirosis is a zoonotic disease, it arises worldwide but it is most frequent in tropical and subtropical zone. It is one of the notifiable and treatable disease. Leptospirosis is a plague caused by species of bacteria called Leptospira the bacteria shed into the nature via urine of infected animals. Rats are the most recurrent source of human sepsis. Rivers are the assumption to be a predominant risk factor for transmission of disease to humans. It possesses an extensive variation of mechanisms that allow them to avoid the host immune system and cause infection. The infection is extremely vast ranging from subclinical to multi organ infection with elevated mortality. It is frequently mild but can be terminal, it is likely to be serious and the serious alignment form known as Weils disease and can easily steer to death. The mingling of renal failure, hemorrhage and jaundice is known as Weils disease. It is the most affection pattern associated with critical leptospirosis. It is accumulating as a serious problem worldwide and superficially existing as co-infections with various unrelated diseases, including malaria and dengue. Laboratory diagnostic tests are not always accessible and usually diagnosis is executed by enzyme linked immunosorbent assay (ELISA) serology and microscopic agglutination test, rapid test are also feasible. The MAT (microscopic agglutination test) is known as Gold standard. Serological tests are most frequently used for the diagnosis of leptospirois. The carcinogenesis of human disease and mechanism of cell membrane injuries which take place mainly due to the occupancy of leptospirosis along with their antigen in host tissues many molecules hand out to the ability of leptospira to invade, colonize and to adhere. In most of the cases antibiotics are preferred to reduce the symptoms of leptospirosis.

Major Forest Changes in Subtropical China since the Last Ice Age

In the subtropical zone of southern China, there was a considerable conversion of forests from deciduous to evergreen broadleaf in the early Holocene. However, the exact timing of this vegetation change and its relationship to climate are still unclear. We examined a high-resolution pollen record collected in the mid-subtropical zone and then performed a correlation with regional data to reconstruct the history of forest ecosystems since the last deglaciation. Our data show that the expansion of the evergreen plant component already occurred at low elevations during the last deglaciation. The subtropical mountain landscape was not recolonized by evergreen forests until the mid-Holocene at about 8.1 ka BP. Based on fossil pollen reconstruction and climate model simulation, we conclude that the primary increase in evergreen components of subtropical ecosystems was triggered by postglacial temperature increase, and that a complete conversion from deciduous to evergreen forest ecosystems did not occur until Holocene winter temperatures and seasonal temperature contrast reached a threshold suitable for the growth and persistence of evergreen tree species.