Effect of Inhibin-B hormone and its relationship with a number of sex hormones in men with Azoospermia

Inhibin-B is produced by sertoli cells and acts as a negative feedback mechanism of follicular stimulation hormone (FSH). In order to evaluate the effect of Inhibin-B in men with Azoospermia, the study measured the concentrations of the following hormones: Inhibin-B, FSH, Luteinizing Hormone Prolactin (PRL), (LH) and Testosterone (Testo). Serum for 30 normal men semen and 50 men with Azoespermia (zero sperm count in sperm). The mean concentration of the hormone Inhibin-B in patients with Azoospermia was (135.7 ±87.6) pg/ml, while in normal men (175.6 ±47.9) Pg / ml with a significant value of the group of healthy patients at a significant level (p≤0.01). The mean serum FSH concentration in patients was (13.7 ± 12.27) lU / L whereas in normal men (4.1 ± 2.84) lU / L, the relationship was significant between patients and healthy at a significant level (P≤0.01) the correlation with the Inhibin-B hormone is reversible relationship. The mean concentration of LH in patients was (12.8±11.87) IU/L and in the healthy (7.9±5.57) IU/L and the relationship between them was significant (P≤0.05) and the correlation with the Inhibin-B hormone is positive relationship. The mean concentration of the Testosterone In the patients was (2.37 ± 1.22) IU / L and in the healthy (5.1 ± 1.96) IU/ L, the relationship was of significant value at P≤0.01 and the correlation with the Inhibin-B hormone is positive relationship, and there is a difference in the mean concentration of PRL between the patients and the healthy where it reaches (8.88 ±5.16) IU / L in patients and (8.03 ± 4.2) IU / L in the healthy. where the relationship was non-significant value between both patients and healthy and the correlation with the Inhibin-B hormone is positive relationship . http://dx.doi.org/10.25130/tjps.24.2019.042

od additive on female reproductive organs and hormones fo AzorubineEffects of in Sprague Dawley rat

The objective of the present study was to assess the effects of various doses of Azorubine which is a food additive on the female reproductive organs in Sprague Dawley rats. Twenty four female Sprgue Dawley rats were divided randomly into 4 equal groups. Group 1(control group), group, 2, 3 and 4 were received Azorubine (5, 10 and 20 mg/kg) orally, daily for 30 days respectively. Blood samples were taken for estimation of white blood cells, red blood cells, hemoglobin and platelets, in addition Luteinizing, follicular stimulation, estrogen and progesterone hormones from the sera. The reproductive hormones levels affected drastically under the effects of different doses of treatment like Luteinizing hormone (0.69±0.25, 0.60±0.75 and 0.55±0.63), Follicular Stimulation hormone (0.17±0.11, 0.13±0.33 and 0.3±0.45), Progesterone hormone (0.50±0.77, 0.14±0.56 and 0.10±0.85), and estrogen hormone (0.45+0.43, 0.30±0.29 and 0.14±0.27) hormones were decreased significantly (P˂0.05) in groups of rats treated with each 5, 10 and 20 mg/kg doses of Azorubine respectively. Histopathologically, the ovaries treated with 5 mg/kg doses of Azorubine showing follicles at the beginning stages of growth with no Graffian follicle while the ovaries with 10 mg/kg doses of Azorubine contain fully grown Graffian follicles with no follicles at various stages as well as those with 20 mg/kg doses of treatment displaying no mature Graffian follicle with many atretic and shrunk follicles. The hematological outcomes are significantly affected by this food additive. The results of this work is concluded that Azorubine can be considered as one of the most important causes of infertility, hormonal disturbances and irregular estrus cycle in the female rat.