Hidden by the name: A new fluorescent pumpkin toadlet from the Brachycephalus ephippium group (Anura: Brachycephalidae)

Species of Brachycephalus has been having taxonomical issues due its morphological similarity and genetic conservatism. Herein, we describe a new species of Brachycephalus from the south Mantiqueira mountain range and semidecidual forests in the municipalities of Mogi das Cruzes, Campinas and Jundiaí, state of São Paulo, Brazil, based on an integrative approach. It can be distinguished from all species of the B. ephippium species group based on morphological characters (especially osteology and head shape), advertisement call and divergence in partial mitochondrial DNA gene sequences (16S). The new species is genetically similar to B. margaritatus and morphologically similar to B. ephippium. It can be differentiated from B. ephippium by the presence of dark faded spots on skull and post-cranial plates, presence of black connective tissue connective tissue scattered over dorsal musculature, parotic plate morphology, smaller snout-vent length (adult SVL: males 13.46–15.92 mm; females 16.04–17.69 mm) and 3% genetic distance. We also present natural history data and discuss the robustness of the integrative approach, geographic distribution, genetic data, behaviour, fluorescence in ontogeny, and conservation status.

Effects of long-term use of the preferential COX-2 inhibitor meloxicam on growing pigs

Meloxicam, a preferential COX-2 inhibitor, is a commonly used NSAID in pigs. Besides having potential side effects on the gastrointestinal tract, this type of drug might potentially affect osteogenesis and chondrogenesis, processes relevant to growing pigs. Therefore, the effects of long-term meloxicam treatment on growing pigs were studied. Twelve piglets (n=6 receiving daily meloxicam 0.4 mg/kg orally from 48 until 110 days of age; n=6 receiving only applesauce (vehicle control)) were subjected to visual and objective gait analysis by pressure plate measurements at several time points. Following euthanasia a complete postmortem examination was performed and samples of the talus and distal tibia, including the distal physis, were collected. Trabecular bone microarchitecture was analysed by microCT scanning, bone stiffness by compression testing and growth plate morphology using light microscopy. Animals were not lame and gait patterns did not differ between the groups. Pathological examination revealed no lesions compatible with known side effects of NSAIDs. Trabecular bone microarchitecture and growth plate morphology did not differ between the two groups. The findings of this in vivo study reduce concerns regarding the long-term use of meloxicam in young, growing piglets.

Unexpectedly high diversity in a small basin: A taxonomic revision of Eurycheilichthys (Siluriformes: Loricariidae), with descriptions of seven new species

ABSTRACT A taxonomic revision of Eurycheilichthys is provided, and seven new species are described in addition to the two previously known species in this genus: E. pantherinus from the upper rio Uruguay, and E. limulus from the upper rio Jacuí. The genus is diagnosed based on the uniquely derived presence of seven branched pectoral-fin rays, and on the possession of reduced filamentous gill rakers in the oral surface of the hyobranchial skeleton, and presence of a distinct fleshy flap along the posterodorsal margin of the pectoral-fin spine in adult males. The seven new species are described from tributaries to the rio Taquari basin, itself a tributary to the rio Jacuí in Rio Grande do Sul State, southern Brazil. The new species are diagnosed based on color variation, abdominal plate morphology, lip size, parieto-supraoccipital shape, the number of dermal plates, the number of teeth, and body proportions. The high diversity and degree of species endemism in a limited area are discussed and compared to other fish groups. Genetic sequences (GenSeq) of mitochondrial and nuclear DNA, distribution maps, an identification key, and illustrations are presented for all species.