Sector subsampling for basal area ratio estimation: an alternative to big BAF sampling

Big basal area factor (big BAF) sampling is a widely used subsampling method to select measure-trees. Several studies have shown big BAF sampling to be an efficient sampling scheme. In this study, we use sector sampling (Smith et al. 2008, For. Sci. 54: 67–76) as an alternative subsample selection method. Based on some simulated mapped stands derived from three balsam fir (Abies balsamea (L.) Mill.) spacing trials in western Newfoundland, we show that sector subsampling is comparable to big BAF sampling in terms of estimated mean basal area ratios and their associated standard errors. Differences between big BAF sampling and sector sampling methods showed less than 1% difference across the three sites. As with big BAF sampling, changes in sample intensity had no significant (p < 0.05) effects on the accuracy of estimating mean biomass to basal area ratios and the resulting estimated mean biomasses per unit area.

Evaluation of Sample Preparation Methods for the Analysis of Reef-Building Corals Using 1H-NMR-Based Metabolomics

The field of metabolomics generally lacks standardized methods for the preparation of samples prior to analysis. This is especially true for metabolomics of reef-building corals, where the handful of studies that were published employ a range of sample preparation protocols. The utilization of metabolomics may prove essential in understanding coral biology in the face of increasing environmental threats, and an optimized method for preparing coral samples for metabolomics analysis would aid this cause. The current study evaluates three important steps during sample processing of stony corals: (i) metabolite extraction, (ii) metabolism preservation, and (iii) subsampling. Results indicate that a modified Bligh and Dyer extraction is more reproducible across multiple coral species compared to methyl tert-butyl ether and methanol extractions, while a methanol extraction is superior for feature detection. Additionally, few differences were detected between spectra from frozen or lyophilized coral samples. Finally, extraction of entire coral nubbins increased feature detection, but decreased throughput and was more susceptible to subsampling error compared to a novel tissue powder subsampling method. Overall, we recommend the use of a modified Bligh and Dyer extraction, lyophilized samples, and the analysis of brushed tissue powder for the preparation of reef-building coral samples for 1H NMR metabolomics.