Stream channelization is a common form of anthropogenic disturbance, whose impacts on cold-water biological communities have received little attention in comparison with the body of work demonstrating its negative effects on lowland, warm-water systems. Furthermore, it has been suggested that physical stream processes, given sufficient temporal scale, are capable of restoring channelized reaches to their natural state; however, empirical evidence of this is lacking. Here, we examine the effects of stream channelization on fish and invertebrate communities and their habitats in disturbed and undisturbed cold-water mountain streams in southern Alberta, 80 years postchannelization. Our results show that negative impacts associated with channelization, such as a significant reduction of deep pools and loss of flow refugia, remain prevalent along impacted reaches, inhibiting the reestablishment of native species across multiple trophic levels. While these findings are applicable for a range of cold-water salmonid populations, we suggest that they may be of special importance to the management of mountain whitefish (Prosopium williamsoni) populations, which may be more sensitive to channelization than other cold-water species.