Effect of Fungus-Growing Termite on Soil CO2 Emission at Termitaria Scale in Dry Evergreen Forest, Thailand

Termites are one of the major contributors to high spatial variability in soil respiration. Although epigeal termite mounds are considered as a point of high CO2 effluxes, the patterns of mound CO2 effluxes are different, especially the mound of fungus-growing termites in a tropical forest. This study quantified the effects of a fungus-growing termite (Macrotermes carbonarius) associated with soil CO2 emission by considering their nesting pattern in dry evergreen forest, Thailand. A total of six mounds of M. carbonarius were measured for CO2 efflux rates on their mounds and surrounding soils in dry and wet seasons. Also, measurement points were investigated for the active underground passages at the top 10% of among efflux rates. The mean rate of CO2 emission from termitaria of M. carbonarius was 7.66 µmol CO2/m2/s, consisting of 2.94 and 9.11 µmol CO2/m2/s from their above mound and underground passages (the rate reached up to 50.00 µmol CO2/m2/s), respectively. While the CO2 emission rate from the surrounding soil alone was 6.86 µmol CO2/m2/s. The results showed that the termitaria of M. carbonarius contributed 8.4% to soil respiration at the termitaria scale. The study suggests that fungus-growing termites cause a local and strong variation in soil respiration through underground passages radiating out from the mounds in dry evergreen forest.

Evaluation of the chemical defense fluids of Macrotermes carbonarius and Globitermes sulphureus as possible household repellents and insecticides

The use of chemical insecticides has had many adverse effects. This study reports a novel perspective on the application of insect-based compounds to repel and eradicate other insects in a controlled environment. In this work, defense fluid was shown to be a repellent and insecticide against termites and cockroaches and was analyzed using gas chromatography-mass spectrometry (GC–MS). Globitermes sulphureus extract at 20 mg/ml showed the highest repellency for seven days against Macrotermes gilvus and for thirty days against Periplaneta americana. In terms of toxicity, G. sulphureus extract had a low LC50 compared to M. carbonarius extract against M. gilvus. Gas chromatography–mass spectrometry analysis of the M. carbonarius extract indicated the presence of six insecticidal and two repellent compounds in the extract, whereas the G. sulphureus extract contained five insecticidal and three repellent compounds. The most obvious finding was that G. sulphureus defense fluid had higher potential as a natural repellent and termiticide than the M. carbonarius extract. Both defense fluids can play a role as alternatives in the search for new, sustainable, natural repellents and termiticides. Our results demonstrate the potential use of termite defense fluid for pest management, providing repellent and insecticidal activities comparable to those of other green repellent and termiticidal commercial products.

Occurrence of poly(hydroxyalkanoate) in the gut homogenate of a phylogenetically higher termite: Macrotermes carbonarius

Gas chromatography analysis of a phylogenetically higher termite-gut homogenate detected the presence of trace amounts of poly(3-hydroxybutyrate) (PHB), a prokaryotic storage material. In addition, the gut homogenate smear, stained with Nile blue A, also suggested the presence of PHB-like granules. Chloroform extracts of both soldier and worker classes of this termite were obtained for further spectroscopic analysis. FTIR, 1H NMR, and 2D 1H-1H NMR analyses confirmed the presence of PHB in both the chloroform extracts. This showed for the first time the occurrence of bacteria capable of accumulating PHB in the termite gut. The results indicated that the physiological environment in the termite gut is suitable for the colonization by PHB-producing bacteria and is probably rich in organic carbon sources, which can be readily assimilated and stored as PHB.Key words: Macrotermes carbonarius, poly(hydroxyalkanoate), PHB, termite