Cafestol and Kahweol: A Review on Their Bioactivities and Pharmacological Properties

Cafestol and kahweol are natural diterpenes extracted from coffee beans. In addition to the effect of raising serum lipid, in vitro and in vivo experimental results have revealed that the two diterpenes demonstrate multiple potential pharmacological actions such as anti-inflammation, hepatoprotective, anti-cancer, anti-diabetic, and anti-osteoclastogenesis activities. The most relevant mechanisms involved are down-regulating inflammation mediators, increasing glutathione (GSH), inducing apoptosis of tumor cells and anti-angiogenesis. Cafestol and kahweol show similar biological activities but not exactly the same, which might due to the presence of one conjugated double bond on the furan ring of the latter. This review aims to summarize the pharmacological properties and the underlying mechanisms of cafestol-type diterpenoids, which show their potential as functional food and multi-target alternative medicine.

A Study and Estimation of Different Distance Measures in Generalized Fuzzy TOPSIS to Improve Ranking Order

Multi criteria decision making (MCDM) is used to solve multiple conflicting criteria. There are different methods available in MCDM out of which TOPSIS is a well- known method to solve precise and imprecise information. In this chapter, triangular fuzzy TOPSIS is considered which has different steps like normalization, weight, finding of positive ideal solution (PIS) and negative ideal solution (NIS), distance between PIS and NIS, calculating relative closeness coefficient (RCC) value and ranking the alternatives. Out of these different steps a distance method is studied. The distance measures are basically used to find the distance between the target alternative and the best and the least alternatives. The most commonly used distance method is Euclidean distance. Many other distance methods are available such as Manhattan, Bit-vector, Hamming, Chebyshev distance, etc. To obtain the appropriate distance, these methods are evaluated. The proposed approach is applied in banking domain to find the suitable user for multi criteria reporting (MCR).

Pathobiology of pulmonary arterial hypertension: understanding the roads less travelled

The pathobiology of pulmonary arterial hypertension (PAH) is complex and incompletely understood. Although three pathogenic pathways have been relatively well characterised, it is widely accepted that dysfunction in a multitude of other cellular processes is likely to play a critical role in driving the development of PAH. Currently available therapies, which all target one of the three well-characterised pathways, provide significant benefits for patients; however, PAH remains a progressive and ultimately fatal disease. The development of drugs to target alternative pathogenic pathways is, therefore, an attractive proposition and one that may complement existing treatment regimens to improve outcomes for patients. Considerable research has been undertaken to identify the role of the less well-understood pathways and in this review we will highlight some of the key discoveries and the potential for utility as therapeutic targets.

Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana

Background: CRISPR-Cas is a recent and powerful edition to the molecular toolbox which allows programmable genome editing. It has been used to modify genes in a wide variety of organisms, but only two alga to date. Here we present a methodology to edit the genome of T. pseudonana, a model centric diatom with both ecological significance and high biotechnological potential, using CRISPR-Cas.Results: A single construct wa assembled using Golden Gate cloning. Two sgRNAs were used to introduce a precise 37nt deletion early in the coding region of the urease gene. A high percentage of bi-allelic mutations (≤ 61.5%) were observed in clones with the CRISPR-Cas construct. Growth of bi-allelic mutants in urea led to a significant reduction in growth rate and cell size compared to growth in nitrate.Conclusions: CRISPR-Cas can precisely and efficiently edit the genome of T. pseudonana. The use of Golden Gate cloning to assemble CRISPR-Cas constructs gives additional flexibility to the CRISPR-Cas method and facilitates modifications to target alternative genes or species.

The Effect of Constructing Multiple-Choice Distractor Items around a Single Target Alternative

The present research sought to determine whether the construction of multiple-choice alternatives based around a critical target answer would facilitate the selection of the target answer. Subjects were given a multiple-choice test consisting of 60 questions, each having four alternatives. Twenty of the 60 questions were the critical questions and were constructed to have no correct answer (i.e., asked nonsense) but appeared legitimate. One of the alternatives for the critical questions was the critical alternative, around which the other three distractor alternatives were derived. This was accomplished by systematically substituting each of the critical alternative's three components with another plausible component. This procedure produced a set of alternatives where the critical alternative was more similar to the other alternatives than they were to each other (i.e., it was the most prototypic). The results of two experiments using ranking and proportion scores showed a response bias effect: subjects selected the critical alternatives more often than would be expected by chance. Further analyses revealed that in lower ability subjects the effect disappeared when the critical alternatives were embedded in sets of distractors which had randomly ordered components. High ability subjects selected the critical alternatives more often than chance regardless of the distractors' component arrangement. The results suggest that test-makers should avoid constructing distractor alternatives around a correct alternative because the information provided in the set of alternatives may influence test-takers to select the target answer without any knowledge of the information being assessed.