Quantitative volumetric analysis of the Golgi apparatus following X-ray irradiation by super-resolution 3D-SIM microscopy

To obtain quantitative volumetric data for the Golgi apparatus after ionizing radiation (IR) using super-resolution three-dimensional structured illumination (3D-SIM) microscopy. Normal human retinal pigment epithelial (RPE) cells were irradiated with X-rays (10 Gy), followed by immunofluorescence staining of the Golgi marker RCAS1. 3D-SIM imaging was performed using DeltaVision OMX version 4 and SoftWoRx 6.1. Polygon rendering and spot signal identification were performed using Imaris 8.1.2. Differences between groups were assessed by Welch’s t test. RCAS1 signals in untreated cells were located adjacent to nuclei and showed a reticular morphology. Upon IR, the area of RCAS1 signals expanded while retaining the reticular morphology. Polygon rendering imaging revealed that the volume of RCAS1 at 48 h post-IR was greater than that for unirradiated cells (93.7 ± 19.0 μm3 vs. 33.0 ± 4.2 μm3, respectively; P < 0.001): a 2.8-fold increase. Spot signal imaging showed that the number of RCAS1 spot signals post-IR was greater than that for unirradiated cells [3.4 ± 0.8 (× 103) versus 1.3 ± 0.2 (× 103), respectively; P < 0.001]: a 2.7-fold increase. This is the first study to report quantitative volumetric data of the Golgi apparatus in response to IR using super-resolution 3D-SIM microscopy.

MANAJEMEN WIRELESS DENGAN MAPPING SSID ACCESS POINT PADA STMIK LOMBOK

The wireless network at STMIK Lombok is connected to the local server network and the internet with the spread of access points in several buildings. In some parts of the building have different strengths and the access point or wireless signal strengths, some have broad and strong signal coverage, but there are also areas that have limited / weak signal coverage, even no wireless or blank-spot signal. This blank spot can be caused by many obstacles, including walls, tiles, trees, and others. Based on observations at STMIK Lombok, wireless signal coverage has not been able to cover the entire campus building area; many are unstable and tend to be weak. Therefore, wireless management research was conducted by mapping the SSID access point which is expected to offer/give a solution so that the wireless signal can cover all STMIK Lombok campus buildings. Wireless management by mapping SSID access points on STMIK Lombok provides a solution for location placement and number of access points to be installed so that the campus area of ​​STMIK Lombok can be covered by wireless networks. Keywords : wireless, wireless network, mapping SSID, access point, MikroTik

The Design of Wireless Communication System in Oil Log Spot

This article that based on the communication technology of 2.4G global free frequency range adopts technical route which can stabilize and reliable work in high—intensity magnetic field and other disturb spot acquisition of signal technology. The technical route is that collecting the spot signal and making the wireless communication, then restoring the signal to logging. The modern information communication in oil log will come true through this process. This technology not only overcome many malpractice which exist in the wired communication but also allow the logging cabin far from well site, which both reduce the cost of logging and improve the work efficiency