Flow Control in Porous Media: From Numerical Analysis to Quantitative μPAD for Ionic Strength Measurements

Microfluidic paper-based analytical devices (µPADs) are a promising technology to enable accurate and quantitative in situ assays. Paper’s inherent hydrophilicity drives the fluids without the need for external pressure sources. However, controlling the flow in the porous medium has remained a challenge. This study addresses this problem from the nature of the paper substrate and its design. A computational fluid dynamic model has been developed, which couples the characteristics of the porous media (fiber length, fiber diameter and porosity) to the fluidic performance of the diffusion-based µPAD sensor. The numerical results showed that for a given porous membrane, the diffusion, and therefore the sensor performance is affected not only by the substrate nature but also by the inlets’ orientation. Given a porous substrate, the optimum performance is achieved by the lowest inlets’ angle. A diffusion-based self-referencing colorimetric sensor was built and validated according to the design. The device is able to quantify the hydronium concentration in wines by comparison to 0.1–1.0 M tartaric acid solutions with a 41.3 mM limit of detection. This research showed that by proper adjustments even the simplest µPADs can be used in quantitative assays for agri-food applications.

Predicting potential areas for the formation of Co-rich ferromanganese crusts in the Canary Islands Seamount Province using multi-criteria GIS analysis

<p>Marine ferromanganese crusts are metal-rich chemical sediments showing an increasing economic interest as potential mineral resources for strategic and critical metals. Formation of Fe-Mn crusts is linked to a series of different factors that favor or limit their genesis on the seabed. The objective of this work is the search of areas for potential formation of these deposits, using data obtained in the Canary Islands Seamount Province. The study has been carried out based on multi-criteria analysis, using a Geographic Information System (ArcGis 10.5, Spatial Analysis and Statistical tools). For this purpose, it has been created a cartographic model, which considers data related to depth, seabed substrate nature and age, slope and exposure to marine bottom currents. This model has been applied to non-sampled areas, contrasting them with data derived from the analysis of samples taken in different oceanographic surveys, in order to establish the correct conclusions. This work has been carried out using bathymetric and geological data shared by the Geological Survey of Spain (IGME) with the MINDeSEA [1] and EMODnet-Geology [2] European projects.</p><p>[1] This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 731166.</p><p>[2] EMODNET-Geology project (EASME/EMFF/2018/1.3.1.8-Lot 1/SI2.811048).</p>

Green Nanocoatings Based on the Deposition of Zirconium Oxide: The Role of the Substrate

Herein, the influence of the substrate in the formation of zirconium oxide monolayer, from an aqueous hexafluorozirconic acid solution, by chemical conversion and by electro-assisted deposition, has been approached. The nanoscale dimensions of the ZrO2 film is affected by the substrate nature and roughness. This study evidenced that the mechanism of Zr-EAD is dependent on the potential applied and on the substrate composition, whereas conversion coating is uniquely dependent on the adsorption reaction time. The zirconium oxide based nanofilms were more homogenous in AA2024 substrates if compared to pure Al grade (AA1100). It was justified by the high content of Cu alloying element present in the grain boundaries of the latter. Such intermetallic active sites favor the obtaining of ZrO2 films, as demonstrated by XPS and AFM results. From a mechanistic point of view, the electrochemical reactions take place simultaneously with the conventional chemical conversion process driven by ions diffusion. Such findings will bring new perspectives for the generation of controlled oxide coatings in modified electrodes used, as for example, in the construction of battery cells; in automotive and in aerospace industries, to replace micrometric layers of zinc phosphate by light-weight zirconium oxide nanometric ones. This study is particularly addressed for the reduction of industrial waste by applying green bath solutions without the need of auxiliary compounds and using lightweight ceramic materials.

Acetonitrile and benzonitrile as versatile amino sources in copper-catalyzed mild electrochemical C–H amidation reactions

Different pathways of C–H transformation depending on the substrate nature and oxidation potential.

New topoisomerase inhibitors: Evaluating the potency of gepotidacin and zoliflodacin in fluoroquinolone-resistant Escherichia coli upon tolC inactivation and differentiating their efflux pump substrate nature.

Inactivating tolC in multidrug-resistant Escherichia coli with differing sequence types and quinolone resistance-determining mutations reveals remarkably potentiated activity of the first-in-class topoisomerase inhibitors gepotidacin and zoliflodacin. Differences between both structurally unrelated compounds in comparison to fluoroquinolones regarding the selectivity of E. coli RND-type transporters, efflux inhibitors, and AcrB porter domain mutations were demonstrated. The findings should reinforce efforts to develop efflux bypassing drugs and provide AcrB targets with critical relevance for this purpose.