In this study we explore the use of collision cross
section distributions to allow comparability of IM-MS data for proteins on
different instruments. We present measurements on seven standard proteins
across three IM-MS configurations, namely an Agilent 6560 IM QToF, a Waters
Synapt G2 possessing a TWIMS cell and a modified Synapt G2 possessing an RF
confining linear field drift cell. Mobility measurements were taken using both
He and N<sub>2</sub> as the drift gases.
To aid comparability across instruments and best assess the
corresponding gas-phase conformational landscapes of the protein ‘standards’ we
present the data in the form of averaged collision cross section distributions.