Improved Bone Cell Adhesion on Ultrafine Grained Titanium and Ti-6A1-4V

(1) One strategy to improve the outcome of orthopedic implants is to use porous implants with the addition of a coating with an antibacterial biomolecule. In this study, we aimed to produce and test the biocompatibility, the osteopromotive (both under normal conditions and under a bacterial challenge with lipopolysaccharide (LPS)) and antibacterial activities of a porous Ti-6Al-4V implant coated with the flavonoid quercitrin in vitro. (2) Porous Ti-6Al-4V implants were produced by 3D printing and further functionalized with quercitrin by wet chemistry. Implants were characterized in terms of porosity and mechanical testing, and the coating with quercitrin by fluorescence staining. Implant biocompatibility and bioactivity was tested using MC3T3-E1 preosteoblasts by analyzing cytotoxicity, cell adhesion, osteocalcin production, and alkaline phosphatase (ALP) activity under control and under bacterial challenging conditions using lipopolysaccharide (LPS). Finally, the antibacterial properties of the implants were studied using Staphylococcus epidermidis by measuring bacterial viability and adhesion. (3) Porous implants showed pore size of about 500 µm and a porosity of 52%. The coating was homogeneous over all the 3D surface and did not alter the mechanical properties of the Young modulus. Quercitrin-coated implants showed higher biocompatibility, cell adhesion, and osteocalcin production compared with control implants. Moreover, higher ALP activity was observed for the quercitrin group under both normal and bacterial challenging conditions. Finally, S. epidermidis live/dead ratio and adhesion after 4 h of incubation was lower on quercitrin implants compared with the control. (4) Quercitrin-functionalized porous Ti-6Al-4V implants present a great potential as an orthopedic porous implant that decreases bacterial adhesion and viability while promoting bone cell growth and differentiation.

Download Full-text

Selective bone cell adhesion on formulations containing carbon nanofibers

Biomaterials ◽

10.1016/s0142-9612(02)00609-9 ◽

2003 ◽

Vol 24 (11) ◽

pp. 1877-1887 ◽

Cited By ~ 287

Author(s):

Rachel L Price ◽

Michael C Waid ◽

Karen M Haberstroh ◽

Thomas J Webster

Keyword(s):

Cell Adhesion ◽

Carbon Nanofibers ◽

Bone Cell

Download Full-text

Studies of bone cell adhesion and proliferation on ion-implanted titanium discs

Australian Journal of Mechanical Engineering ◽

10.1080/14484846.2009.11464581 ◽

2009 ◽

Vol 7 (1) ◽

pp. 77-82

Author(s):

P S Sreejith ◽

P K D V Yarlagadda

Keyword(s):

Cell Adhesion ◽

Bone Cell ◽

Cell Adhesion And Proliferation ◽

Ion Implanted

Download Full-text

Ion implantation induced nanotopography on titanium and bone cell adhesion

Applied Surface Science ◽

10.1016/j.apsusc.2014.03.118 ◽

2014 ◽

Vol 310 ◽

pp. 24-30 ◽

Cited By ~ 10

Author(s):

Iñigo Braceras ◽

Carolina Vera ◽

Ana Ayerdi-Izquierdo ◽

Roberto Muñoz ◽

Jaione Lorenzo ◽

...

Keyword(s):

Cell Adhesion ◽

Ion Implantation ◽

Bone Cell

Download Full-text

A probabilistic approach to measure the strength of bone cell adhesion to chemically modified surfaces

Annals of Biomedical Engineering ◽

10.1007/bf02738550 ◽

1997 ◽

Vol 25 (1) ◽

pp. 190-203 ◽

Cited By ~ 35

Author(s):

Alireza Rezania ◽

Carson H. Thomas ◽

Kevin E. Healy

Keyword(s):

Cell Adhesion ◽

Probabilistic Approach ◽

Bone Cell ◽

Chemically Modified ◽

Chemically Modified Surfaces ◽

Modified Surfaces

Download Full-text

Effects of nano-engineered surfaces on osteoblast adhesion, growth, differentiation, and apoptosis

Proceedings of the Institution of Mechanical Engineers Part N Journal of Nanomaterials Nanoengineering and Nanosystems ◽

10.1177/2397791419886778 ◽

2019 ◽

Vol 234 (1-2) ◽

pp. 59-66

Author(s):

Raheleh Miralami ◽

John G Sharp ◽

Fereydoon Namavar ◽

Curtis W Hartman ◽

Kevin L Garvin ◽

...

Keyword(s):

Cell Adhesion ◽

Bone Formation ◽

Zirconium Oxide ◽

Ion Beam ◽

Bone Cell ◽

Calcium Deposition ◽

Deposition Technique ◽

Ion Beam Assisted Deposition ◽

Osteoblast Adhesion ◽

Glass Surfaces

Modifying implant surfaces to improve their biocompatibility by enhancing osteoblast activation, growth, differentiation, and induction of greater bone formation with stronger attachments should result in improved outcomes for total joint replacement surgeries. This study tested the hypothesis that nano-structured surfaces, produced by the ion beam-assisted deposition method, enhance osteoblast adhesion, growth, differentiation, bone formation, and maturation. The ion beam-assisted deposition technique was employed to deposit zirconium oxide films on glass substrates. The effects of the ion beam-assisted deposition technique on cellular functions were investigated by comparing adhesion, proliferation, differentiation, and apoptosis of the human osteosarcoma cell line SAOS-2 on coated versus uncoated surfaces. Ion beam-assisted deposition nano-coatings enhanced initial cell adhesion assessed by the number of 4′,6-diamidino-2-phenylindole–stained nuclei on zirconium oxide nano-coated surfaces compared to glass surfaces. This nano-modification also increased cell proliferation as measured by mitochondrial dehydrogenase activity. Moreover, the ion beam-assisted deposition technique improved cell differentiation as determined by the formation of mineralized bone nodules and by the rate of calcium deposition, both of which are in vitro indicators of the successful bone formation. However, programmed cell death assessed by Annexin V staining and flow cytometry was not statistically significantly different between nano-surfaces and glass surfaces. Overall, the results indicate that nano-crystalline zirconium oxide surfaces produced by the ion beam-assisted deposition technique are superior to uncoated surfaces in supporting bone cell adhesion, proliferation, and differentiation. Thus, surface properties altered by the ion beam-assisted deposition technique enhanced bone formation and may increase the biocompatibility of bone cell–associated surfaces.

Download Full-text

Biomolecular Surface Engineering of Materials for Controlling Bone Cell Adhesion and Spreading

MRS Proceedings ◽

10.1557/proc-530-99 ◽

1998 ◽

Vol 530 ◽

Author(s):

A. Rezania ◽

K. E. Healy

Keyword(s):

Cell Adhesion ◽

Spectroscopic Ellipsometry ◽

Photoelectron Spectroscopy ◽

Surface Engineering ◽

Bone Cells ◽

Bone Cell ◽

Focal Contact ◽

Biomaterial Surfaces ◽

Cell Incubation ◽

Strength Of Adhesion

AbstractModel biomaterial surfaces were modified with a peptide that contained a -RGD- (Arg-GlyAsp) sequence, unique to bone sialoprotein, to determine its effect on strength of adhesion, spreading, and focal contact formation of primary bone-derived cells. Peptide surfaces were fabricated by using a heterobifunctional crosslinker to graft the peptide to surfaces (quartz and silicon). Contact angle measurements, spectroscopic ellipsometry, and X-ray photoelectron spectroscopy were used to confirm the chemistry and thickness of the overlayers. Furthermore, spectroscopic ellipsometry was used to estimate the density of immobilized peptide on metal oxide surfaces. A radial flow apparatus was used to measure the strength of adhesion on peptide grafted surfaces. Following 20 min of cell incubation, the strength of cell adhesion was significantly (p<0.05) higher on the -RGD- compared to -RGE- (control) surfaces. The mean area of cells contacting the -RGD- surface was significantly (p<0.05) higher than -RGE- surfaces. Vinculin staining revealed formation of focal contact patches on the periphery of bone cells incubated for 4 hr on the -RGD- surfaces; however, cells seeded on the -RGE- grafted surfaces formed little or no focal contacts. The methods of peptide immobilization utilized in this study can be applied to medical devices, biosensors, and diagnostic assays that require specificity in cell adhesion.

Download Full-text