Identification of Species in Mixed Microbial Populations using MALDI-TOF MS

ABSTRACT The aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by Maldi-Tof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.

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MALDI-TOF/MS identification of species from the Acinetobacter baumannii (Ab) group revisited: inclusion of the novel A. seifertii and A. dijkshoorniae species

Clinical Microbiology and Infection ◽

10.1016/j.cmi.2016.11.020 ◽

2017 ◽

Vol 23 (3) ◽

pp. 210.e1-210.e9 ◽

Cited By ~ 43

Author(s):

M. Marí-Almirall ◽

C. Cosgaya ◽

P.G. Higgins ◽

A. Van Assche ◽

M. Telli ◽

...

Keyword(s):

Acinetobacter Baumannii ◽

The Novel ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Identification Of Species ◽

Tof Ms

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Use of MALDI-TOF MS in diagnostic microbiology for identification of species that conventional methods usually fail to identify

Clinical & Biomedical Research ◽

10.4322/2357-9730.90933 ◽

2019 ◽

Vol 39 (2) ◽

pp. 171-174

Author(s):

Max Roberto Batista Araújo ◽

Luan Freitas Lana ◽

Luisa Ferreira Seabra

Keyword(s):

Maldi Tof Ms ◽

Maldi Tof ◽

Identification Of Species ◽

Conventional Methods ◽

Diagnostic Microbiology ◽

Tof Ms

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Rapid microbial identifications by MALDI-TOF – Viva la revolution!

Microbiology Australia ◽

10.1071/ma10111 ◽

2010 ◽

Vol 31 (3) ◽

pp. 111

Author(s):

Stephen A Neville ◽

Iain B Gosbell

Keyword(s):

Mass Spectrometry ◽

Bacterial Identification ◽

Maldi Tof Ms ◽

Microbial Identification ◽

Maldi Tof ◽

Flight Mass Spectrometry ◽

Identification Of Species ◽

Phenotypic Methods ◽

Diagnostic Microbiology ◽

Tof Ms

Although the application of mass spectrometry to bacterial identification was proposed as far back as 1975 by John Anhalt, it was not until 1987 when Professor Franz Hillenkamp and Dr Michael Karas first pioneered Matrix Assisted Laser Desorption/Ionisation Time-of-Flight mass spectrometry (MALDI-TOF MS) for truly rapid microbial identification in diagnostic microbiology laboratories. Conventional phenotypic methods of identifying isolates of bacteria, yeasts and filamentous fungi require hours to several days to complete, depending on the type of organism involved, and may be prone to error due to bias or inexperience. Even the more recent molecular innovations have their limitations that place them outside the scope and budget of many routine laboratories. However, MALDI-TOF MS technology has changed the way we think about microbial identifications and strain differentiation by providing results from plate to name in approximately five minutes for one isolate and around 90 minutes for 60 isolates at under $2 an identification. It is likely to be particularly helpful clinically for identification of species in positive blood culture broths and for organisms that are hard to identify quickly.

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