A computer-assisted two-dimensional gel electrophoresis approach for studying the variations in protein expression related to an induced functional repression of NFkB in lymphoblastoid cell lines

2007 ◽  
pp. 437-446
Author(s):  
Raymond Joubert-Caron ◽  
Jean Feuillard ◽  
Sylvie Kohanna ◽  
Florence Poirier ◽  
Jean-Pierre Le Car ◽  
...  
Keyword(s):  
Protein Expression ◽  
Cell Lines ◽  
Gel Electrophoresis ◽  
Computer Assisted ◽  
Lymphoblastoid Cell Lines ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

scholarly journals Demonstration of a third structurally distinct human Ia beta chain by two-dimensional gel electrophoresis.

1982 ◽  
Vol 156 (2) ◽  
pp. 652-657 ◽  
Author(s):  
R W Karr ◽  
C C Kannapell ◽  
J A Stein ◽  
T C Fuller ◽  
R J Duquesnoy ◽  
...  
Keyword(s):  
Cell Lines ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Beta Chain ◽  
Two Dimensional Gel Electrophoresis ◽  
Hla Dr

Previous studies have indicated that HLA-DR homozygous cell lines express two Ia alpha and Ia beta chains that combine to form at least two Ia molecules. This report demonstrates by two-dimensional gel electrophoresis the existence of a third structurally distinct human Ia beta chain on DR2 and DR5 cell lines. This suggests that at least five separate genes control the expression of Ia molecules on HLA-DR homozygous cell lines.

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