The rostral level of origin of sympathetic neurons in the chick embryo, studied in tissue culture

1979 ◽  
Vol 154 (4) ◽  
pp. 557-561 ◽  
Author(s):  
D. F. Newgreen
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Sympathetic Neurons

The Uptake and Localization of Catecholamines in Chick Embryo Sympathetic Neurons in Tissue Culture

1969 ◽  
Vol 4 (3) ◽  
pp. 677-691
Author(s):  
J. M. ENGLAND ◽  
M. N. GOLDSTEIN
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Ganglion Cells ◽  
Sympathetic Neurons ◽  
Neuronal Cell ◽  
Cell Types ◽  
Axon Terminals ◽  
Dense Core Granules ◽  
Insoluble Compounds ◽  
Dorsal Root Ganglion Cells

The uptake of exogenous [3H]dopamine, [3H]norepinephrine,[3H]epinephrine by dissociated chick embryo sympathetic neurons growing in tissue culture was studied by autoradiography. The neurons, growing in a medium containing nerve growth factor, rapidly and specifically took up all three catecholamines for at least 60 days, while no uptake was observed in several other cell types, including satellite cells and chick dorsal-root ganglion cells. The uptake was dependent on the concentration of the catecholamine and the duration of the pulse and was inhibited by cocaine and several sympathomimetic amines. Labelling was visualized only with fixatives which react with catecholamines to form water-insoluble compounds. Autoradiographs showed that the label was much denser over the axons than the cell bodies. The label was distributed uniformly along the axons and did not seem to be preferentially localized at the axon terminals or varicosities which contain aggregates of dense core granules. These observations indicate that a large portion of the exogenous 3[H]catecholamine is localized in an extragranular compartment and suggest that the differentiated function of the sympathetic neuronal cell membrane, which plays an important role in uptake, is retained after prolonged tissue culture.

FURTHER STUDIES ON THE DUAL REQUIREMENT FOR PHENYLALANINE AND TYROSINE IN TISSUE CULTURE

1961 ◽  
Vol 39 (5) ◽  
pp. 925-932 ◽  
Author(s):  
Helen J. Morton ◽  
Joseph F. Morgan
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Chick Embryo ◽  
Heart Cells ◽  
Present System ◽  
Chick Heart ◽  
High Concentrations ◽  
Chick Embryo Heart ◽  
Embryo Heart ◽  
Related Compounds

Seventeen structurally related compounds were tested for their ability to substitute for phenylalanine or tyrosine in the nutrition of chick embryo heart fragments. DL-Alanyl-DL-phenylalanine replaced phenylalanine. All other compounds had negligible effects, and most were toxic at high concentrations. β-Phenylserine, a phenylalanine antagonist, actually prolonged the survival of chick heart cells but only if both phenylalanine and tyrosine were present. Similarly, optimal reversal of β-phenylserine toxicity was dependent on the presence of both amino acids. Although phenylalanine and tyrosine are not interconvertible in the present system, it has been shown that three phenylalanine antagonists, p-fluorophenylalanine, β-2-thienylalanine, and β-phenylserine, can be identified by their relationship to tyrosine, rather than to phenylalanine.

scholarly journals Mushrooms as a source of substances with antiviral activity

2014 ◽  
Vol 16 (2) ◽  
pp. 215-220 ◽  
Author(s):  
Martyna Kandefer-Szerszeń ◽  
Zbigniew Kawecki ◽  
Bogusław Sałata ◽  
Maria Witek
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Antiviral Activity ◽  
Vaccinia Virus ◽  
Fungal Species ◽  
Water Extracts ◽  
Boletus Edulis ◽  
Vaccinia Viruses ◽  
Embryo Fibroblasts ◽  
Armillariella Mellea

Water extracts the fructifications of 56 species of fungi were examined as a source of antiviral substances with activity against VS and vaccinia viruses. Extracts from 16 fungal species exhibited the antiviral activity. Water extracts from <i>Boletus edulis</i> active against vaccinia virus and extract from <i>Armillariella mellea</i> active against VS virus are particularly worth nothing. Both of them in applied concentrations were not toxic in chick embryo fibroblasts tissue culture.

scholarly journals THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS

1967 ◽  
Vol 32 (1) ◽  
pp. 89-112 ◽  
Author(s):  
George G. Rose
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Mechanical System ◽  
Environmental Conditions ◽  
The Other ◽  
Cover Slip

A self-contained mechanical system for circulating nutrient fluid through 12 tissue culture chambers is described in detail. This system utilizes nonperforated cellophane membranes in the chambers which separate the circulating nutrient from the tissue culture environments. The nutrient, therefore, is dialyzed through the cellophane of each chamber; some cell products are retained in the microenvironment between the closely apposed cellophane and cover slip, whereas the other cell products move from chamber to chamber in the circulating nutrient. The resultant environmental conditions directed by the circumfusion systems are highly favorable for maintaining the differentiation of chick embryo tissues over protracted periods; a number of micrographs are shown.

Use of Desiccated Chick Embryo in Tissue Culture Technique

Nature ◽  
1940 ◽  
Vol 146 (3688) ◽  
pp. 30-31
Author(s):  
P. R. PEACOCK ◽  
R. I. SHUKOFF
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Culture Technique ◽  
Tissue Culture Technique
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