In this study, it was investigated to increase the lipid yield of the
microalgae Schizochytrium sp., by applying different cell disruption
methods. Therefore, acid treatment with HCl, osmotic shock, enzyme
applications and ultrasonic homogenizer were tried in this algae species
combined with the Bligh and Dyer and Soxhlet methods as an alternative
to classical lipid extraction methods. As a result of the study, the
highest lipid value (21.72 ± 0.74%) was obtained in enzyme application
with Bligh and Dyer method (BDE). The cell disruption processes
increased the lipid yield compared to the control groups. The highest
PUFA DHA was found in the range of 4.58 ± 2.44-19.25 ± 0.09%, and the
highest value was observed in the BDE group. Highest SFA was palmitic
acid. Effective results were observed in the Bligh and Dyer applied
groups in terms of both total lipid and total fatty acids. In cell
disruption methods, particularly in enzyme and HCl extraction, good
results were obtained in terms of fatty acids. The highest total fatty
acids and the highest lipid content were detected in the Bligh and Dyer
enzyme (BDE). Enzyme applications are also advantageous because of being
environmentally friendly. Lipid health indices such as n-6/n-3,
PUFA/SFA, Atherogenicity index (AI), Thrombogenicity index (TI) and
hypocholesterolemic/hypercholesterolemic ratios (HH) were almost
favorable. With this study, an appropriate lipid extraction methods were
determined to provide an economical and environmental friendly
suggestion for future studies to be used in areas such as food, feed and
cosmetics.
Effect of different cell disruption methods on lipid yield of
Schizochytrium
sp.
