Alterations in health-related fatty acids in buffalo milk after processing to traditional dairy products

Milk provides some beneficial fatty acids which in dairy processing are subjected to pasteurization and fermentation. With the aim to assess such changes, aliquot parts of milk from 12 buffaloes were pooled and processed to germinated yoghurt and brined cheese, and to non-germinated curd – the respective samples of raw and dairy material subjected to lipid analysis. The results show that in cheese positive and negative changes are generally balanced, rumenic acid decreasing and other CLAs altered but not total CLA and PUFA; omega ratio and atherogenicity index worsened to little extent, due to adverse change in n-3, myristic and lauric acid. In yoghurt and curd CLA dramatically decreased, excluding rumenic acid; but vaccenic acid increased, though total trans isomers decreased; the worsened n-6/n-3 ratio and atherogenicity index is mostly because of the adverse effect on PUFAn-3 but also on myristic and lauric acid. In all products SFA and MUFA did not change, including palmitic, stearic, and oleic acid. It can be concluded that the decrease of CLA in yoghurt and curd is partially compensated by the increase in the vaccenic acid, while cheese making altered individual isomers but not groups of beneficial acids.

Traditional meat preparations in the Balkans region

The aim of this study was to determine the fatty acid (FA) profile and trans fatty acid (TFA) content of the Balkan’s traditional meat preparations. Twenty-four meat preparations were examined: fresh sausages, ćevapčići, pljeskavica and hamburger. Gas chromatography of FA and TFA was used. Trans fat was higher than the recommended value of 0.5% in ćevapčići, pljeskavica and hamburger (0.54-0.62%) and lower in fresh sausages (0.26%). There were significant differences (P < 0.05) in the atherogenicity index (AI), which was lower in fresh sausages (0.58) and ćevapčići (0.64) and pljeskavica (0.64) and highest in hamburger (0.77). Concerning thrombogenicity indices (TI), there was a significant difference (P < 0.05), being the lowest in fresh sausages (1.42), ćevapčići (1.28) and pljeskavica (1.40) and the highest in hamburger (1.82). AI and TI of traditional meat preparations were higher than recommended indices, 0.5 and 1.0, respectively, and so are not desirable for health protection. The n-6/n-3 ratio was significantly (P < 0.05) lower in pljeskavica (10.76) and hamburger (7.30) compared with ćevapčići (22.25) and fresh sausages (28.41). Promotion of the Mediterranean diet requires changes in the food systems and public health policies to improve overall diet quality of individuals, communities, and populations.

Fatty acids profile, atherogenic and thrombogenic health lipid indices in the meat of lambs that received canola grain

This study evaluated the fatty acid composition and qualitative characteristics of meat from lambs feed in feedlot with increasing levels of canola grain. Meat obtained from 27 lambs fed 0, 8 and 16% inclusion of canola grain were analyzed. There was a reduction in the content of saturated fatty acids (SFA): lauric, heptadecanoic, and stearic; and unsaturated fatty acids (UFA): palmitoleic, oleic, and eicosatrienoic, with the increasing levels of canola grain. There was a linear reduction for the ω3 acid and the ω3: ω6 ratio, while the ω6: ω3 ratio increased. But the values observed for this ratio (ω6: ω3) were lower than 4, which is considered a satisfactory value. There was a slight increase (~2%) for the thrombogenicity index and atherogenicity index with the inclusion of canola grain. There was a reduction in the hypocholesterolemic: hypercholesterolemic ratio, with mean values of 2.09 for the diet with 0% inclusion, and 2.06 for 8 and 16% inclusion of canola. Although the inclusion of canola grain for lambs decreased the content of some UFA, reflecting alterations in the correlated nutritional properties, there is a reduction of SFA. The results for instrumental analysis, proximate composition, and sensory acceptance of the lamb meat were similar among the treatments.

Studying the effect of the developed technology on the chemical composition of yogurt made from camel milk

This work aimed to conduct a comparative analysis that helps to identify the effect of the developed technology on the chemical composition of drinking yogurts made from Australian and Kazakhstani dromedary camel milk. Camel milk taken from Kazakhstan and Australia has been processed into drinking yogurt and its amino, fatty acid, vitamin, and mineral content was assayed. These identifications enabled us to compare how our developed technology is suitable for both milk types. The results of determination can be interpreted as follows. The essential and non-essential amino acid content in Kazakhstani yogurt was significantly higher compared to Australian yogurt. Aspartic and Glutamic acids were not identified in Kazakhstani yogurt. As a counterpart, Lysine and Histidine were not found in Australian yogurt. The fatty acid results demonstrated that Linoleic acid in Kazakhstani yogurt was significantly higher than in Australian yogurt, and there was more Linolenic acid in Australian yogurt than in Kazakhstani yogurt. The atherogenicity index for Kazakhstani yogurt was at a low level (0.045 %) compared to Australian yogurt (1.90 %). The ratios of omega 6 and omega 3 in Kazakhstani yogurt were 16 % greater than in Australian yogurt. Thiamine level in Kazakhstani yogurt was lower compared to Australian by up to 57 %. However, Riboflavin results in both samples were identical. The Calcium, Potassium, Sodium, and Phosphorus contents in Australian yogurt are defined as 5, 34, 34, and 30 % respectively compared to Kazakhstani yogurt. Nevertheless, Magnesium (47 %) and Iron (60 %) levels were lower in Australian yogurt than in Kazakhstani yogurt. These study results could be useful as preliminary work for scientists and producers of gerodiet products, who intend to work with camel milk as a geroprotector

Effect of different cell disruption methods on lipid yield of Schizochytrium sp.

In this study, it was investigated to increase the lipid yield of the microalgae Schizochytrium sp., by applying different cell disruption methods. Therefore, acid treatment with HCl, osmotic shock, enzyme applications and ultrasonic homogenizer were tried in this algae species combined with the Bligh and Dyer and Soxhlet methods as an alternative to classical lipid extraction methods. As a result of the study, the highest lipid value (21.72 ± 0.74%) was obtained in enzyme application with Bligh and Dyer method (BDE). The cell disruption processes increased the lipid yield compared to the control groups. The highest PUFA DHA was found in the range of 4.58 ± 2.44-19.25 ± 0.09%, and the highest value was observed in the BDE group. Highest SFA was palmitic acid. Effective results were observed in the Bligh and Dyer applied groups in terms of both total lipid and total fatty acids. In cell disruption methods, particularly in enzyme and HCl extraction, good results were obtained in terms of fatty acids. The highest total fatty acids and the highest lipid content were detected in the Bligh and Dyer enzyme (BDE). Enzyme applications are also advantageous because of being environmentally friendly. Lipid health indices such as n-6/n-3, PUFA/SFA, Atherogenicity index (AI), Thrombogenicity index (TI) and hypocholesterolemic/hypercholesterolemic ratios (HH) were almost favorable. With this study, an appropriate lipid extraction methods were determined to provide an economical and environmental friendly suggestion for future studies to be used in areas such as food, feed and cosmetics.

Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination

The total lipid content and lipidic profile of seaweeds harvested in the North Coast and purchased in Portugal were determined in this paper. The amount of total lipids in the different species of seaweeds varied between 0.7 ± 0.1% (Chondrus crispus) and 3.8 ± 0.6% (Ulva spp.). Regarding the fatty acid content, polyunsaturated fatty acids (PUFA) ranged between 0–35%, with Ulva spp. presenting the highest amount; monounsaturated fatty acids (MUFA) varied between 19 and 67%; and saturated fatty acids (SFA) were predominant in C. crispus (45–78%) and Gracilaria spp. (36–79%). Concerning the nutritional indices, the atherogenicity index (AI) was between 0.4–3.2, the thrombogenicity index (TI) ranged from 0.04 to 1.95, except for Gracilaria spp., which had a TI of 7.6, and the hypocholesterolemic/hypercholesterolemic ratio (HH) values ranged between 0.88–4.21, except for Gracilaria spp., which exhibited values between 0.22–9.26. The n6/n3 ratio was below 1 for most of the species evaluated, except for Ascophyllum nodosum, which presented a higher value, although below 2. Considering the PUFA/SFA ratio, seaweeds presented values between 0.11–1.02. The polycyclic aromatic hydrocarbons (PAHs) and aliphatic hydrocarbons (AHCs) contamination of seaweeds under study was also quantified, the values found being much lower than the maximum levels recommended for foodstuff.

The risk of developing cardiovascular complications in employees who operate and maintain communication facilities based on wired and wireless technologies

Introduction. Heart disease has remained the leading cause of death in the world for the past 20 years. The development and progression of cardiovascular diseases, along with genetic, somatic, behavioral, socioeconomic, environmental, and other risk factors, are significantly affected by unfavorable industrial and professional aspects: physical-vibration, noise, temperature disturbance; ergonomic - inactivity, and monotonous work, physical overstrain, chemical, biological, stress, etc. The primary pathogenetic mechanism leading to the development of CHD and fatal complications - myocardial infarction, strokes, thrombosis, and other diseases of the cardiovascular system is atherosclerosis. The study aimed to assess the risk of developing cardiovascular complications in employees who operate and maintain communication facilities based on wired and wireless technologies Materials and methods. An in-depth examination of the health status of 50 employees of the service for the operation of radio equipment and communications was conducted. Blood serum levels of glucose, cholesterol, triglycerides, HDL, and LDL were determined, and the atherogenicity index was calculated. The probability of total risk of cardiovascular complications and five-year cardiovascular risk was calculated for all the examined patients according to the European SCORE scale and the ASCORE rating scale. The "Vascular age" was also calculated. Results. Based on the analysis of lipid metabolism indicators, a high cardiovascular risk was identified in 40% of the examined patients. Increased values of the atherogenicity index were already observed in middle-aged people (45-60 years). Analysis of the data obtained using the SCORE and ASCORE assessment scales revealed a high risk of developing cardiovascular complications in middle-aged (45-60 years) and elderly (61-74 years) individuals. The excess of the vascular age in comparison with the real (passport) age was established in middle-aged (45-60 years) and elderly (61-74 years) individuals, on average, 7 (p<0.001) and 5 (p=0.026) years, respectively. Conclusion. The most pronounced changes in lipid metabolism and the risk of cardiovascular risk were in people of the most working age (45-60). In this regard, it is necessary to develop preventive measures aimed at cardioscreening to detect early signs of health disorders, prevent the development of cardiovascular complications, and the formation of groups at increased risk of diseases.

Evaluation of Lipid Profile and Atherogenic Index of Plasma in Patients with Type 2 Diabetes (AIP = log TG/HDL-c)

Aims: The objective of our study was to evaluate the lipid profile and the plasma atherogenicity index obtained from the log (TG / HDL-c) in diabetics patients. Study Design: This is a comparative and analytical study. Place and Duration of Study: Sample: MARC SANKALE Centre at the Abass Ndao Hospital in Dakar (Senegal), CHNU/Fann Biochemistry Laboratory, from June 2018 to November 2019. Controls: For each patient, a witness of the same sex and the same age ± 2 years was recruited. Methodology: The lipid balance parameters were assayed using enzymatic techniques with the Cobas c311 system (Roche Diagnostics, Switzerland). Plasma atherogenicity indices for each patient were calculated (CT / HDL-c, LDL / HDL-c and Log (TG / HDL-c)). Data analysis was performed using XLSTAT software and a p value <0.05 was considered to be a statistically significant difference. Results: Our study concerned 100 subjects with type 2 diabetes. The average age was 50.5 ± 10.80 years old and the sex ratio was 0.58. Evaluation of lipid parameters had shown an increase in diabetic subjects compared to controls for total cholesterol (2.30 g / l) and LDL-cholesterol (1.40 g / l) with significant differences (p < 0.001). We also found that 11% of patients had a CT / HDL-c ratio > 4.5, while 8% had an LDL-c / HDL-c ratio > 3.5 and 26% of patients had a log (Tg / HDL- c) > 0.21. Conclusion: Lipid disturbances constitute significant abnormalities in type 2 diabetic subjects and would predispose them to cardiovascular complications. However, IAP = log (TG / HDL-c) could be considered the most sensitive predictor of cardiovascular risk.

Risk of metabolic syndrome in river transport workers

In river transport workers metabolic disorders begin to appear after 10 years of living in Yakutia. High values of blood pressure, atherogenicity index and young age of river transport workers with metabolic syndrome (MS), than in the comparable population group with MS, indicate the influence of working conditions and climate on the acceleration of metabolic disorders.

ОСНОВНЫЕ ГЕМАТОЛОГИЧЕСКИЕ И МЕТАБОЛИЧЕСКИЕ ПОКАЗАТЕЛИ КРОВИ У ЛЕТНОГО СОСТАВА НА РАЗНЫХ СРОКАХ СЛУЖБЫ В УСЛОВИЯХ АРКТИКИ

A series of peripheral blood characteristics were assessed in military pilots at service in Arctic immediately upon arrival at their dislocation site and by the end of the third, sixth, and twelfth month of service. The metabolic characteristics included total cholesterol (Ch), cholesterol of high, low, and very low density lipoproteins (HDL, LDL, and VLDL), triglycerides (TG), glucose, bilirubin, and atherogenicity index. Differential white blood cell and red blood cell counts were determined in stained blood smears. The parameters were compared at each term of service between groups of pilots having diagnoses “healthy” (control), spinal osteochondrosis, gastroduodenitis, myocardiodystrophy, neurovascular dystonia, and marginal arterial hypertension, which were below levels judged as contraindications against professional duties. In the last three of the above conditions, statistically significant increases lymphocytes (9.1–11.4%); and red blood cells (7.5–11.3%). These changes were most expressed upon marginal hypertension. The results are discussed in the context of pilots’ adaptation to stress caused by Arctic conditions.