Designing dna probe from hpv 18 and 58 in e6 region for sensing element in the development of genosensor based gold nanoparticles

2021 ◽  
Author(s):  
F. Nadhirah Jaapar ◽  
N.A. Parmin ◽  
N. Hamidah A Halim ◽  
Uda Hashim ◽  
Subash C.B. Gopinath ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dna Probe ◽  
Sensing Element ◽  
Hpv 18

Colorimetric multiplexed analysis of mercury and silver ions by using a unimolecular DNA probe and unmodified gold nanoparticles

2012 ◽  
Vol 4 (10) ◽  
pp. 3320 ◽  
Author(s):  
Zhihe Qing ◽  
Xiaoxiao He ◽  
Kemin Wang ◽  
Zhen Zou ◽  
Xue Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Ions ◽  
Dna Probe ◽  
Multiplexed Analysis

A hairpin-type DNA probe for direct colorimetric detection of endonuclease activity and inhibition based on the deaggregation of gold nanoparticles

2019 ◽  
Vol 186 (2) ◽  
Author(s):  
Fuming Sang ◽  
Guoliang Li ◽  
Jun Li ◽  
Jianxin Pan ◽  
Zhizhou Zhang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Dna Probe ◽  
Endonuclease Activity

Development of an impedimetric aflatoxin M1 biosensor based on a DNA probe and gold nanoparticles

2011 ◽  
Vol 26 (9) ◽  
pp. 3806-3811 ◽  
Author(s):  
Erhan Dinçkaya ◽  
Özer Kınık ◽  
Mustafa Kemal Sezgintürk ◽  
Çağrı Altuğ ◽  
Aylin Akkoca
Keyword(s):  
Gold Nanoparticles ◽  
Dna Probe ◽  
Aflatoxin M1

A Highly Sensitive Electrochemical Impedimetric Nanobiosensor for Dust Mite Antigen Der p2 Detection

2011 ◽  
Author(s):  
I-Jiuan Bau ◽  
Gou-Jen Wang
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Deposition ◽  
Barrier Layer ◽  
Electrochemical Impedance ◽  
High Sensitivity ◽  
Sensing Element ◽  
Self Assembled Monolayer ◽  
Highly Sensitive ◽  
Der P2 ◽  
Dust Mite

The group 2 allergen, Der p2, has been reported to activate innate toll-like receptors (TLRs) on respiratory epithelial cells and thus aggravate respiratory diseases. In this study, a high sensitive nanobiosensor based on a 3D sensing element that has uniformly deposited gold nanoparticles for the detection of the dust mite antigen Der p2 is proposed. The barrier layer of an anodic aluminum oxide (AAO) film is used as the template in this highly sensitive nanobiosensor fabricated with a reducing agent and stabilizer-free method. Electrochemical deposition is utilized to synthesize uniformly distributed gold nanoparticles on the surface of the barrier layer. The size and the distribution density of the nanoparticles can be well controlled by the potential applied during electrochemical deposition. Following this procedure, monoclonal antibodies were immobilized against the dust mite antigen Der p2 by the gold nanoparticles through the 11-MUA (11-mercaptoundecanoic acid), EDC (1-Ethyl-3-(3-dimethyl-aminopropyl)-carbodiimide)/NHS (N-hydroxysuccinimide) self-assembled monolayer approach. The proposed nanobiosensor was successfully used to examine the Der p2 down to a concentration of 1pg/mL through the electrochemical impedance spectroscopy analysis. The high sensitivity of the proposed 3D nanobiosensor can be attributed to the high intensity and uniformity of the Au nanoparticles on the sensor. The proposed nanobiosensor would be useful for the fast detection of rare molecules in a solution.

Enhancement of the immobilization and discrimination of DNA probe on a biosensor using gold nanoparticles

2001 ◽  
Vol 46 (13) ◽  
pp. 1074-1077 ◽  
Author(s):  
Zhao Hongqiu ◽  
Lin Lin ◽  
Tang Ji’an ◽  
Duan Mingxing ◽  
Jiang Long
Keyword(s):  
Gold Nanoparticles ◽  
Dna Probe

DETERMINATION OF AMINOGLYCOSIDE ANTIBIOTICS BY A COLORIMETRIC METHOD BASED ON THE AGGREGATION OF GOLD NANOPARTICLES

NANO ◽  
2013 ◽  
Vol 08 (04) ◽  
pp. 1350037 ◽  
Author(s):  
RUIYONG WANG ◽  
SHUMIN FAN ◽  
RUIQIANG WANG ◽  
RUI WANG ◽  
HUANJING DOU ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Incubation Time ◽  
Color Change ◽  
Limit Of Detection ◽  
Colorimetric Method ◽  
Aminoglycoside Antibiotics ◽  
Sensing Element ◽  
Experimental Conditions ◽  
Experimental Parameters

A sensitive and selective colorimetric biosensor for determination of gentamicin, amikacin and tobramycin was proposed with the unmodified gold nanoparticles (GNPs) as the sensing element. Gentamicin, amikacin and tobramycin can rapidly induce the aggregation of gold nanoparticles and is accompanied by a color change from red to blue. The concentration of gentamicin, amikacin and tobramycin can be determined by using UV-Vis spectrometer. The experimental parameters were optimized with regard to pH, incubation time and the concentration of the GNPs. Under optimal experimental conditions, the linear range of the colorimetric sensor for gentamicin/amikacin/tobramycin were 2.67–33.93 ng mL-1, 13.33–66.67 ng mL-1 and 20–180 ng mL-1, respectively. The corresponding limit of detection (3σ) was 0.354 ng mL-1, 0.999 ng mL-1 and 0.579 ng mL-1, respectively. This assay was simple and used to detect aminoglycoside antibiotics in milk and medicine products.

Fluorescence recognition of double-stranded DNA based on the quenching of gold nanoparticles to a fluorophore labeled DNA probe

The Analyst ◽  
2016 ◽  
Vol 141 (20) ◽  
pp. 5829-5834 ◽  
Author(s):  
Xiangmin Miao ◽  
Zongbing Li ◽  
Liansheng Ling
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescent Sensor ◽  
Dna Probe ◽  
Specific Recognition ◽  
Double Stranded Dna

This work described an ultrasensitive fluorescent sensor for sequence-specific recognition of dsDNA based on the quenching of gold nanoparticles (AuNPs) to a fluorophore labeled DNA probe.

scholarly journals A Simple and Ultrasensitive Colorimetric Biosensor for Anatoxin-a Based on Aptamer and Gold Nanoparticles

Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1526
Author(s):  
Duy-Khiem Nguyen ◽  
Chang-Hyun Jang
Keyword(s):  
Gold Nanoparticles ◽  
High Selectivity ◽  
Color Change ◽  
Specific Binding ◽  
Color Variation ◽  
Dna Aptamer ◽  
Nacl Solution ◽  
Sensing Element ◽  
Promising Alternative ◽  
Dispersion State

Here, we designed a simple, rapid, and ultrasensitive colorimetric aptasensor for detecting anatoxin-a (ATX-a). The sensor employs a DNA aptamer as the sensing element and gold nanoparticles (AuNPs) as probes. Adsorption of the aptamer onto the AuNP surface can protect AuNPs from aggregation in NaCl solution, thus maintaining their dispersion state. In the presence of ATX-a, the specific binding of the aptamer with ATX-a results in a conformational change in the aptamer, which facilitates AuNP aggregation and, consequently, a color change of AuNPs from red to blue in NaCl solution. This color variation is directly associated with ATX-a concentration and can be easily measured using a UV/Vis spectrophotometer. The absorbance variation is linearly proportional to ATX-a concentration across the concentration range of 10 pM to 200 nM, with a detection limit of 4.45 pM and high selectivity against other interferents. This strategy was successfully applied to the detection of ATX-a in lake water samples. Thus, the present aptasensor is a promising alternative method for the rapid detection of ATX-a in the environment.

An organic electrochemical transistor for determination of microRNA21 using gold nanoparticles and a capture DNA probe

2018 ◽  
Vol 185 (9) ◽  
Author(s):  
Jing Peng ◽  
Tao He ◽  
Yulian Sun ◽  
Yawen Liu ◽  
Qianqian Cao ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dna Probe ◽  
Electrochemical Transistor
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