Aims:
The study aims to develop advanced antibacterial agents as nanoparticles instead of antibiotics due to the emergence of antimicrobial resistance.
Background:
Pseudomonas aeruginosa is capable of causing many diseases, including severe bacterial pneumonia. There is a need for an efficient antibacterial agent to kill these pathogens.
Objective:
The objective of the study is to synthesize advanced antibacterial agents as nanoparticles for biomedical applications that can play a vital role in killing Gram-negative bacteria (Pseudomonas aeruginosa).
Method:
A novel fabricated growth of hydrophilic spiky gold nanoparticles (SGNPs) via reduction method is reported.
Results:
The surface plasmon resonance peak of the synthesized SGNPs was tuned under the near-infrared range. The SGNPs have anisotropic and spiky morphology with 68 nm size and -58 mV surface charge. They are pure, possessing adsorption similar to the organic material. Pseudomonas aeruginosa treated with synthesized SGNPs showed 60% bacterial death at the concentration of 100 μM.
Conclusion:
This work consists of the novel synthesis of SGNPs via a safe and simple reduction method. The synthesized SGNPs exhibit strong antibacterial activity against the Gram-negative bacteria Pseudomonas aeruginosa measured using a microplate assay test. The result showed that these SGNPs are ideal for biomedical applications.