A novel spectrofluorimetric method for determination of Lomefloxacin adopting on Zinc (II) chelation strategy: Application in human plasma

Luminescence ◽  
2021 ◽  
Author(s):  
Tamer Z. Attia ◽  
Mahmoud A. Omar ◽  
Deena A. M. Nour El‐Deen ◽  
Asmaa Mohamed Abbas ◽  
Abobakr A. Mohamed
Keyword(s):  
Human Plasma ◽  
Spectrofluorimetric Method

scholarly journals Spectrofluorimetric Determination of Warfarin Sodium by Using N1-Methylnicotinamide Chloride as a Fluorigenic Agent

2005 ◽  
Vol 88 (2) ◽  
pp. 455-461 ◽  
Author(s):  
Mohamed A El Dawy ◽  
Mokhtar M Mabrouk ◽  
Riad A El Barbary
Keyword(s):  
Aqueous Solutions ◽  
Human Plasma ◽  
Plasma Samples ◽  
Spiked Human Plasma ◽  
Human Plasma Samples ◽  
Warfarin Sodium ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Methylene Groups

Abstract A spectrofluorimetric method is described for the determination of drugs containing active methylene groups adjacent to carbonyl groups. The method was applied successfully to the determination of warfarin sodium in laboratory-prepared mixtures, in commercial tablets, and in spiked human plasma samples. Finally, the method was applied to the determination of the steady-state concentration of warfarin sodium in the blood of a hospitalized patient. The method involves the reaction of warfarin sodium with 0.2 ml (0.4 × 10−3M) N1-methylnicotinamide chloride reagent in the presence of 3 mL 1.0N NaOH and cooling in ice for 8 min, followed by adjustment of the pH to 2.0, using formic acid and heating for 4 min, whereby a highly fluorescent reaction product is produced. The optimal wavelengths of excitation and emission were determined by using a synchronous wavelength search and found to be 284 and 354 nm, respectively. The standard curves were linear over a concentration range of 50–1500 ng/mL in both aqueous solutions and spiked human plasma samples. The mean recoveries (± standard deviation) were 101.157 (±1.33) and 95.73 (±1.88%) for aqueous solutions and spiked human plasma samples, respectively. The method showed good specificity and precision. The proposed method is simple and economical because of its minimal instrumentation and chemicals requirements. Nevertheless, it is highly sensitive, specific, and reproducible. Accordingly, it is suitable for quality-control applications, drug monitoring, and bioavailability and bioequivalency studies.

Selective Spectrofluorimetric Method with Enhanced Sensitivity for Determination of Silodosine in Dosage Form and Human Plasma. Application to Stability Studies and Content Uniformity Testing

2016 ◽  
Vol 27 (2) ◽  
pp. 473-482 ◽  
Author(s):  
Mahmoud A. Omar ◽  
Abdel-Maaboud I. Mohamed ◽  
Sayed M. Derayea ◽  
Mohamed A. Hammad ◽  
Abobakr A. Mohamed
Keyword(s):  
Human Plasma ◽  
Dosage Form ◽  
Content Uniformity ◽  
Stability Studies ◽  
Enhanced Sensitivity ◽  
Spectrofluorimetric Method ◽  
Content Uniformity Testing

scholarly journals Utility of 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole for development of a highly sensitive stability indicating spectrofluorimetric method for determination of salmeterol xinafoate; application to human plasma

RSC Advances ◽  
2017 ◽  
Vol 7 (71) ◽  
pp. 44773-44779 ◽  
Author(s):  
Mahmoud A. Omar ◽  
Mohamed A. Hammad ◽  
Mohamed Awad
Keyword(s):  
Reaction Mechanism ◽  
Human Plasma ◽  
Stability Indicating ◽  
Salmeterol Xinafoate ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive

Suggested reaction mechanism between salmeterol xinafoate and NBD-Cl.

Innovative computationally designed-spectrofluorimetric method for determination of modafinil in tablets and human plasma

Talanta ◽  
2021 ◽  
pp. 122890
Author(s):  
Noha M. Hosny ◽  
Mohammed I. Gadallah ◽  
Rosa M. Gomila ◽  
Wesam S. Qayed
Keyword(s):  
Human Plasma ◽  
Spectrofluorimetric Method

Micellar-Enhanced Spectrofluorimetric Method for Quantification of Diclofenac Potassium in Pure Form, in Pharmaceutical Preparations and Human Plasma

2021 ◽  
Vol 58 (6) ◽  
pp. 427-434
Author(s):  
Muhammad Naeem Khan ◽  
Irum ◽  
Saba Gul ◽  
Muslima ◽  
Muhammad Mursaleen
Keyword(s):  
Human Plasma ◽  
Limit Of Detection ◽  
Sodium Dodecyl ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Products ◽  
Pure Form ◽  
Fluorescence Signal ◽  
Spectrofluorimetric Method ◽  
Relative Standard

Abstract A rapid, simple and economical spectrofluorimetric method for the determination of diclofenac potassium in pure form, in pharmaceutical preparations and in human plasma has been developed. The method is based on the enhancement of the fluorescence signal of diclofenac potassium by the addition of sodium dodecyl sulphate in McIvaine buffer with a pH of 5. Different experimental conditions such as buffer type, pH, type and concentration of surfactants were investigated. The fluorescence intensity of the solution was recorded at 361 nm after excitation at 243 nm. The method shows linearity in the concentration range of 0.2 μg mL–1–10 μg mL–1 with a good correlation coefficient of 0.997. The relative standard deviation value was 3.62 (n = 7). The limit of detection and limit of quantification were calculated to be 2.84 × 10–3 μg mL–1 and 9.47 × 10–3 μg mL-1, respectively. The effect of excipients and co-administrated drugs was investigated and no interference was observed. The method was successfully applied for the determination of diclofenac potassium in pure form, in pharmaceutical products and in human plasma. The percentage recoveries obtained ranged from 100.25% to 102.16% for pure form and 97.50% to 102.00% for pharmaceutical products and from 98.50% to 101.67% for human plasma.

scholarly journals Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing Agent

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Hany W. Darwish ◽  
Ahmed H. Bakheit ◽  
Ali Saber Abdelhameed ◽  
Amer S. AlKhairallah
Keyword(s):  
Human Plasma ◽  
Fluorescence Intensity ◽  
Limit Of Detection ◽  
Biological Fluids ◽  
Great Success ◽  
Limit Of Quantification ◽  
Human Plasma And Urine ◽  
Spectrofluorimetric Method ◽  
Fluorescence Characteristics

An impressively simple and precise spectrofluorimetric procedure was established and validated for ponatinib (PTB) quantitation in biological fluids such as human plasma and human urine. This method depends on examining the fluorescence characteristics of PTB in a micellar system of Cremophor RH 40 (Cr RH 40). Cr RH 40 enhanced the intrinsic fluorescence of PTB distinctly in aqueous water. The fluorescence spectra of PTB was recorded at 457 nm following its excitation at 305 nm. Maximum fluorescence intensity was attained by addition of 0.7 mL of Cr RH 40 and one mL of phosphate buffer to PTB aliquots and then dilution with distilled water. There is a linear relationship between the fluorescence intensity of PTB and its concentration over the range 5–120 ngmL−1, with limit of detection and limit of quantification equal to 0.905 ngmL−1and 2.742 ngmL−1, respectively. The accuracy and the precisions of the proposed method were checked and gave adequate results. The adopted method was applied with a great success for PTB quantitation in different biological matrices (spiked human plasma and urine) giving high recovery values.

Micellar‐based spectrofluorimetric method for the selective determination of ledipasvir in the presence of sofosbuvir: application to dosage forms and human plasma

Luminescence ◽  
2019 ◽  
Vol 35 (4) ◽  
pp. 486-492 ◽  
Author(s):  
Mohamed A. Abdel‐Lateef ◽  
Ramadan Ali ◽  
Mahmoud A. Omar ◽  
Sayed M. Derayea
Keyword(s):  
Human Plasma ◽  
Dosage Forms ◽  
Selective Determination ◽  
Spectrofluorimetric Method
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