Characterization of group C meningococcal polysaccharide by light-scattering spectroscopy. III. Determination of molecular weight, radius of gyration, and translational diffusional coefficient

Biopolymers ◽  
1978 ◽  
Vol 17 (2) ◽  
pp. 251-265 ◽  
Author(s):  
Y. Tsunashima ◽  
K. Moro ◽  
B. Chu ◽  
T. Y. Liu
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Radius Of Gyration ◽  
Light Scattering Spectroscopy

A Progress in Development and Application of Size Exclusion Chromatograph/Multi-Angle Laser Light Scattering

2014 ◽  
Vol 936 ◽  
pp. 1597-1602
Author(s):  
Xue Yang ◽  
Ze Quan Liu ◽  
Qiang Zhang
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Laser Light ◽  
Laser Light Scattering ◽  
Size Exclusion

This paper reveals the development and significance of size exclusion chromatograph/multi-angle laser light scattering(SEC-MALLS) for determination of the molecular weight and characterization of the molecular weight distribution. This technque has been applied in many aspects, such as biology, polymer and carboxylate surfactant. It has made a big contribution to study the property of macromolecular substances.

scholarly journals Application of Dynamic and Static Light Scattering for Size and Shape Characterization of Small Extracellular Nanoparticles in Plasma and Ascites of Ovarian Cancer Patients

2021 ◽  
Vol 22 (23) ◽  
pp. 12946
Author(s):  
Ksenija Kogej ◽  
Darja Božič ◽  
Borut Kobal ◽  
Maruša Herzog ◽  
Katarina Černe
Keyword(s):  
Ovarian Cancer ◽  
Light Scattering ◽  
Spherical Particles ◽  
Radius Of Gyration ◽  
Non Invasive ◽  
Peritoneal Washing ◽  
Angular Dependency ◽  
Shape Characterization ◽  
Further Development

In parallel to medical treatment of ovarian cancer, methods for the early detection of cancer tumors are being sought. In this contribution, the use of non-invasive static (SLS) and dynamic light scattering (DLS) for the characterization of extracellular nanoparticles (ENPs) in body fluids of advanced serous ovarian cancer (OC) and benign gynecological pathology (BP) patients is demonstrated and critically evaluated. Samples of plasma and ascites (OC patients) or plasma, peritoneal fluid, and peritoneal washing (BP patients) were analyzed. The hydrodynamic radius (Rh) and the radius of gyration (Rg) of ENPs were calculated from the angular dependency of LS intensity for two ENP subpopulations. Rh and Rg of the predominant ENP population of OC patients were in the range 20–30 nm (diameter 40–60 nm). In thawed samples, larger particles (Rh mostly above 100 nm) were detected as well. The shape parameter ρ of both particle populations was around 1, which is typical for spherical particles with mass concentrated on the rim, as in vesicles. The Rh and Rg of ENPs in BP patients were larger than in OC patients, with ρ ≈ 1.1–2, implying a more elongated/distorted shape. These results show that SLS and DLS are promising methods for the analysis of morphological features of ENPs and have the potential to discriminate between OC and BP patients. However, further development of the methodology is required.

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