Real-time in situ monitoring of freely suspended and immobilized cell cultures based on mid-infrared spectroscopic measurements

2001 ◽  
Vol 77 (2) ◽  
pp. 174-185 ◽  
Author(s):  
Martin Rhiel ◽  
Paul Ducommun ◽  
Ivan Bolzonella ◽  
Ian Marison ◽  
Urs von Stockar
Keyword(s):  
Real Time ◽  
Cell Cultures ◽  
In Situ Monitoring ◽  
Immobilized Cell ◽  
Mid Infrared ◽  
Spectroscopic Measurements ◽  
Infrared Spectroscopic ◽  
Freely Suspended

scholarly journals Towards Real-Time In-Situ Mid-Infrared Spectroscopic Ellipsometry in Polymer Processing

2021 ◽  
Author(s):  
Alexander Ebner ◽  
Robert Zimmerleiter ◽  
Kurt Hingerl ◽  
Markus Brandstetter
Keyword(s):  
Real Time ◽  
Spectroscopic Ellipsometry ◽  
Quantum Cascade Lasers ◽  
Polymer Processing ◽  
In Situ Monitoring ◽  
Acquisition Time ◽  
Angle Of Incidence ◽  
Mid Infrared ◽  
Ethylene Vinyl Alcohol

Recent developments in mid-infrared (MIR) spectroscopic ellipsometry enabled by quantum cascade lasers (QCLs) resulted in a drastic improvement in signal-to-noise ratio compared to conventional thermal emitter based instrumentation. Thus, it was possible to reduce the acquisition time for high-resolution broadband ellipsometric spectra from multiple hours to less than 1 second. This opens up new possibilities for real-time in-situ ellipsometry in polymer processing. To highlight these evolving capabilities we demonstrate the benefits of a QCL based MIR ellipsometer by investigating single and multilayered polymer films. The molecular structure and reorientation of a 2.5m thin biaxially oriented polyethylene terephtalate film is monitored during a stretching process lasting 24.5 s to illustrate the perspective of ellipsometric measurements in dynamic processes. In addition, a polyethylene/ethylene vinyl alcohol/polyethylene multilayer film is investigated at continuously varying angle of incidence ( 0∘ – 50∘) in 17.2 s, highlighting an unprecedented sample throughput for the technique of varying angle spectroscopic ellipsometry in the MIR spectral range. The obtained results underline the superior spectral and temporal resolution of QCL ellipsometry and qualify this technique as suitable method for advanced in-situ monitoring in polymer processing.

scholarly journals Towards Real-Time In-Situ Mid-Infrared Spectroscopic Ellipsometry in Polymer Processing

Polymers ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 7
Author(s):  
Alexander Ebner ◽  
Robert Zimmerleiter ◽  
Kurt Hingerl ◽  
Markus Brandstetter
Keyword(s):  
Real Time ◽  
Spectroscopic Ellipsometry ◽  
Quantum Cascade Lasers ◽  
Polymer Processing ◽  
In Situ Monitoring ◽  
Acquisition Time ◽  
Angle Of Incidence ◽  
Mid Infrared ◽  
Ethylene Vinyl Alcohol

Recent developments in mid-infrared (MIR) spectroscopic ellipsometry enabled by quantum cascade lasers (QCLs) have resulted in a drastic improvement in signal-to-noise ratio compared to conventional thermal emitter based instrumentation. Thus, it was possible to reduce the acquisition time for high-resolution broadband ellipsometric spectra from multiple hours to less than 1 s. This opens up new possibilities for real-time in-situ ellipsometry in polymer processing. To highlight these evolving capabilities, we demonstrate the benefits of a QCL based MIR ellipsometer by investigating single and multilayered polymer films. The molecular structure and reorientation of a 2.5 µm thin biaxially oriented polyethylene terephthalate film is monitored during a stretching process lasting 24.5 s to illustrate the perspective of ellipsometric measurements in dynamic processes. In addition, a polyethylene/ethylene vinyl alcohol/polyethylene multilayer film is investigated at a continuously varying angle of incidence (0∘– 50∘) in 17.2 s, highlighting an unprecedented sample throughput for the technique of varying angle spectroscopic ellipsometry in the MIR spectral range. The obtained results underline the superior spectral and temporal resolution of QCL ellipsometry and qualify this technique as a suitable method for advanced in-situ monitoring in polymer processing.

scholarly journals A sample cell for the study of enzyme-induced carbonate precipitation at the grain-scale and its implications for biocementation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jennifer Zehner ◽  
Anja Røyne ◽  
Pawel Sikorski
Keyword(s):  
Real Time ◽  
Laser Scanning Microscopy ◽  
In Situ Monitoring ◽  
Confocal Laser ◽  
Precipitation Process ◽  
Successful Implementation ◽  
Detailed Knowledge ◽  
Carbonate Precipitation ◽  
Sample Cell

AbstractBiocementation is commonly based on microbial-induced carbonate precipitation (MICP) or enzyme-induced carbonate precipitation (EICP), where biomineralization of $$\text {CaCO}_{3}$$ CaCO 3 in a granular medium is used to produce a sustainable, consolidated porous material. The successful implementation of biocementation in large-scale applications requires detailed knowledge about the micro-scale processes of $$\text {CaCO}_{3}$$ CaCO 3 precipitation and grain consolidation. For this purpose, we present a microscopy sample cell that enables real time and in situ observations of the precipitation of $$\text {CaCO}_{3}$$ CaCO 3 in the presence of sand grains and calcite seeds. In this study, the sample cell is used in combination with confocal laser scanning microscopy (CLSM) which allows the monitoring in situ of local pH during the reaction. The sample cell can be disassembled at the end of the experiment, so that the precipitated crystals can be characterized with Raman microspectroscopy and scanning electron microscopy (SEM) without disturbing the sample. The combination of the real time and in situ monitoring of the precipitation process with the possibility to characterize the precipitated crystals without further sample processing, offers a powerful tool for knowledge-based improvements of biocementation.

scholarly journals Sensitive In Situ Monitoring of a Recombinant Bioluminescent Yersinia enterocolitica Reporter Mutant in Real Time on Camembert Cheese

2002 ◽  
Vol 68 (11) ◽  
pp. 5737-5740 ◽  
Author(s):  
Ariel Maoz ◽  
Ralf Mayr ◽  
Geraldine Bresolin ◽  
Klaus Neuhaus ◽  
Kevin P. Francis ◽  
...  
Keyword(s):  
Real Time ◽  
Yersinia Enterocolitica ◽  
Photon Emission ◽  
Photon Counting ◽  
In Situ Monitoring ◽  
Charge Coupled Device ◽  
Reading Frame ◽  
Intensified Charge Coupled Device ◽  
Camembert Cheese

ABSTRACT Bioluminescent mutants of Yersinia enterocolitica were generated by transposon mutagenesis using a promoterless, complete lux operon (luxCDABE) derived from Photorhabdus luminescens, and their production of light in the cheese environment was monitored. Mutant B94, which had the lux cassette inserted into an open reading frame of unknown function was used for direct monitoring of Y. enterocolitica cells on cheeses stored at 10°C by quantifying bioluminescence using a photon-counting, intensified charge-coupled device camera. The detection limit on cheese was 200 CFU/cm2. Bioluminescence of the reporter mutant was significantly regulated by its environment (NaCl, temperature, and cheese), as well as by growth phase, via the promoter the lux operon had acquired upon transposition. At low temperatures, mutant B94 did not exhibit the often-reported decrease of photon emission in older cells. It was not necessary to include either antibiotics or aldehyde in the food matrix in order to gain quantitative, reproducible bioluminescence data. As far as we know, this is the first time a pathogen has been monitored in situ, in real time, in a “real-product” status, and at a low temperature.

In-situ monitoring of carbon dioxide emissions from a diesel engine using a mid-infrared optical fibre sensor

2011 ◽  
Author(s):  
Elfed Lewis ◽  
John Clifford ◽  
Colin Fitzpatrick ◽  
Gerard Dooly ◽  
Weizhong Zhao ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Diesel Engine ◽  
Optical Fibre ◽  
Carbon Dioxide Emissions ◽  
In Situ Monitoring ◽  
Mid Infrared ◽  
Optical Fibre Sensor ◽  
Fibre Sensor

Fast, Real-Time, In Situ Monitoring of Solar Ultraviolet Radiation Using Sunlight-Driven Photoresponsive Liquid Crystals

2018 ◽  
Vol 6 (6) ◽  
pp. 1701337 ◽  
Author(s):  
Jiajun Tian ◽  
Yanrong He ◽  
Juntao Li ◽  
Jie Wei ◽  
Gangqiang Li ◽  
...  
Keyword(s):  
Liquid Crystals ◽  
Ultraviolet Radiation ◽  
Real Time ◽  
In Situ Monitoring ◽  
Solar Ultraviolet Radiation ◽  
Solar Ultraviolet

Multifunctional 3D electrode platform for real-time in situ monitoring and stimulation of cardiac tissues

2018 ◽  
Vol 112 ◽  
pp. 149-155 ◽  
Author(s):  
Ning Zhang ◽  
Flurin Stauffer ◽  
Benjamin R. Simona ◽  
Feng Zhang ◽  
Zhao-Ming Zhang ◽  
...  
Keyword(s):  
Real Time ◽  
In Situ Monitoring ◽  
Cardiac Tissues ◽  
3D Electrode ◽  
Stimulation Of
Sign in / Sign up

Export Citation Format

Share Document